Circulating

Impaired Glc tolerance and hyperinsulinemia are a hallmark of type 2 diabetes (T2D) and are associated with an altered innate and adaptive immune response. In this study, we used a high-fat diet (HFD)-induced model of pre-diabetes to explore the pathological implications of altered innate lymphoid c...

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Autores principales:	Vuyolwethu Mxinwa, Phiwayinkosi V. Dludla, Tawanda M. Nyambuya, Bongani B. Nkambule
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/bed6630e01ff4507adaef7999e0f2462
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spelling	oai:doaj.org-article:bed6630e01ff4507adaef7999e0f24622021-12-02T07:35:16ZCirculating1753-42591753-426710.1177/17534259211053634https://doaj.org/article/bed6630e01ff4507adaef7999e0f24622021-10-01T00:00:00Zhttps://doi.org/10.1177/17534259211053634https://doaj.org/toc/1753-4259https://doaj.org/toc/1753-4267Impaired Glc tolerance and hyperinsulinemia are a hallmark of type 2 diabetes (T2D) and are associated with an altered innate and adaptive immune response. In this study, we used a high-fat diet (HFD)-induced model of pre-diabetes to explore the pathological implications of altered innate lymphoid cell (ILC) profiles in a state of impaired Glc tolerance. Sixteen male C57BL/6 mice were randomized to receive two experimental diets ( n = 8 per group), low-fat (LFD), and HFD for 8–13 wk. We evaluated the levels of circulating innate lymphoid cells and their respective cytokines following HFD-feeding. The HFD group had impaired Glc tolerance, elevated insulin levels, and increased total cholesterol levels. Notably, the levels of circulating ILC1s were elevated following 13 wk of HFD-feeding. Moreover, the levels of TNF-α were decreased, but there were no changes in IFN-γ levels. Lastly, the levels of circulating ILC2s and ILC3s were comparable between the HFD and LFD group. The findings demonstrated that short-term HFD-feeding increases postprandial blood Glc, total cholesterol and insulin levels. However, the metabolic changes did not alter ILC2 and ILC3 levels and their respective cytokine profiles.Vuyolwethu MxinwaPhiwayinkosi V. DludlaTawanda M. NyambuyaBongani B. NkambuleSAGE PublishingarticleImmunologic diseases. AllergyRC581-607ENInnate Immunity, Vol 27 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607
spellingShingle	Immunologic diseases. Allergy RC581-607 Vuyolwethu Mxinwa Phiwayinkosi V. Dludla Tawanda M. Nyambuya Bongani B. Nkambule Circulating
description	Impaired Glc tolerance and hyperinsulinemia are a hallmark of type 2 diabetes (T2D) and are associated with an altered innate and adaptive immune response. In this study, we used a high-fat diet (HFD)-induced model of pre-diabetes to explore the pathological implications of altered innate lymphoid cell (ILC) profiles in a state of impaired Glc tolerance. Sixteen male C57BL/6 mice were randomized to receive two experimental diets ( n = 8 per group), low-fat (LFD), and HFD for 8–13 wk. We evaluated the levels of circulating innate lymphoid cells and their respective cytokines following HFD-feeding. The HFD group had impaired Glc tolerance, elevated insulin levels, and increased total cholesterol levels. Notably, the levels of circulating ILC1s were elevated following 13 wk of HFD-feeding. Moreover, the levels of TNF-α were decreased, but there were no changes in IFN-γ levels. Lastly, the levels of circulating ILC2s and ILC3s were comparable between the HFD and LFD group. The findings demonstrated that short-term HFD-feeding increases postprandial blood Glc, total cholesterol and insulin levels. However, the metabolic changes did not alter ILC2 and ILC3 levels and their respective cytokine profiles.
format	article
author	Vuyolwethu Mxinwa Phiwayinkosi V. Dludla Tawanda M. Nyambuya Bongani B. Nkambule
author_facet	Vuyolwethu Mxinwa Phiwayinkosi V. Dludla Tawanda M. Nyambuya Bongani B. Nkambule
author_sort	Vuyolwethu Mxinwa
title	Circulating
title_short	Circulating
title_full	Circulating
title_fullStr	Circulating
title_full_unstemmed	Circulating
title_sort	circulating
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/bed6630e01ff4507adaef7999e0f2462
work_keys_str_mv	AT vuyolwethumxinwa circulating AT phiwayinkosivdludla circulating AT tawandamnyambuya circulating AT bonganibnkambule circulating
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Circulating

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