Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium

Hyperosmolarity under physiological conditions resulting from lumen contents is a big challenge to the normal function of intestinal epithelium. Betaine is a potent osmolyte which is mostly studied in kidney. This study was aimed to gain insight into the osmoprotectant role of betaine in intestinal...

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Autores principales:	Shuyi Xu, Shiyi Lu, Haichao Wang, Sisi Li, Jie Feng
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Betaine Hyperosmotic stress ROS Apoptosis Autophagy Porcine intestinal epithelium Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/bed66b43166840609552ebce28f425e7
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spelling	oai:doaj.org-article:bed66b43166840609552ebce28f425e72021-11-14T04:31:23ZProtective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium1756-464610.1016/j.jff.2021.104838https://doaj.org/article/bed66b43166840609552ebce28f425e72021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1756464621004874https://doaj.org/toc/1756-4646Hyperosmolarity under physiological conditions resulting from lumen contents is a big challenge to the normal function of intestinal epithelium. Betaine is a potent osmolyte which is mostly studied in kidney. This study was aimed to gain insight into the osmoprotectant role of betaine in intestinal epithelium under hyperosmotic condition with intestinal porcine epithelial cells (IPEC-J2) and piglets. The result showed that betaine could attenuate the hyperosmolarity-induced reduction of cell viability after treating 12 h. Betaine ameliorates hyperosmotic stress in intestinal epithelium via decreasing reactive oxygen species (ROS) with the alleviation of cell shrinkage. Moreover, betaine prevented mitochondria from membrane collapse and alleviated mitochondrial-related apoptosis induced by hyperosmolarity in vivo and vitro. In addition, betaine ameliorated autophagy caused by hyperosmolarity by up-regulation LC3 II and down-regulation p62. In summary, betaine protects porcine small intestinal epithelium from hyperosmotic stress by alleviating cell shrinkage, ROS accumulation as well as apoptosis and autophagy.Shuyi XuShiyi LuHaichao WangSisi LiJie FengElsevierarticleBetaineHyperosmotic stressROSApoptosisAutophagyPorcine intestinal epitheliumNutrition. Foods and food supplyTX341-641ENJournal of Functional Foods, Vol 87, Iss , Pp 104838- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Betaine Hyperosmotic stress ROS Apoptosis Autophagy Porcine intestinal epithelium Nutrition. Foods and food supply TX341-641
spellingShingle	Betaine Hyperosmotic stress ROS Apoptosis Autophagy Porcine intestinal epithelium Nutrition. Foods and food supply TX341-641 Shuyi Xu Shiyi Lu Haichao Wang Sisi Li Jie Feng Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium
description	Hyperosmolarity under physiological conditions resulting from lumen contents is a big challenge to the normal function of intestinal epithelium. Betaine is a potent osmolyte which is mostly studied in kidney. This study was aimed to gain insight into the osmoprotectant role of betaine in intestinal epithelium under hyperosmotic condition with intestinal porcine epithelial cells (IPEC-J2) and piglets. The result showed that betaine could attenuate the hyperosmolarity-induced reduction of cell viability after treating 12 h. Betaine ameliorates hyperosmotic stress in intestinal epithelium via decreasing reactive oxygen species (ROS) with the alleviation of cell shrinkage. Moreover, betaine prevented mitochondria from membrane collapse and alleviated mitochondrial-related apoptosis induced by hyperosmolarity in vivo and vitro. In addition, betaine ameliorated autophagy caused by hyperosmolarity by up-regulation LC3 II and down-regulation p62. In summary, betaine protects porcine small intestinal epithelium from hyperosmotic stress by alleviating cell shrinkage, ROS accumulation as well as apoptosis and autophagy.
format	article
author	Shuyi Xu Shiyi Lu Haichao Wang Sisi Li Jie Feng
author_facet	Shuyi Xu Shiyi Lu Haichao Wang Sisi Li Jie Feng
author_sort	Shuyi Xu
title	Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium
title_short	Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium
title_full	Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium
title_fullStr	Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium
title_full_unstemmed	Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium
title_sort	protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/bed66b43166840609552ebce28f425e7
work_keys_str_mv	AT shuyixu protectiveeffectandmechanismofbetaineagainsthyperosmoticstressinporcineintestinalepithelium AT shiyilu protectiveeffectandmechanismofbetaineagainsthyperosmoticstressinporcineintestinalepithelium AT haichaowang protectiveeffectandmechanismofbetaineagainsthyperosmoticstressinporcineintestinalepithelium AT sisili protectiveeffectandmechanismofbetaineagainsthyperosmoticstressinporcineintestinalepithelium AT jiefeng protectiveeffectandmechanismofbetaineagainsthyperosmoticstressinporcineintestinalepithelium
_version_	1718429983094865920

Protective effect and mechanism of betaine against hyperosmotic stress in porcine intestinal epithelium

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