A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation
Abstract Owing to the growing recognition of the gut microbiota as a main partner of human health, we are expecting that the number of indications for fecal microbiota transplantation (FMT) will increase. Thus, there is an urgent need for standardization of the entire process of fecal transplant pro...
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Nature Portfolio
2019
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oai:doaj.org-article:bed83d0e2df642338ed18bee829abe8e2021-12-02T15:09:37ZA Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation10.1038/s41598-019-45173-42045-2322https://doaj.org/article/bed83d0e2df642338ed18bee829abe8e2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45173-4https://doaj.org/toc/2045-2322Abstract Owing to the growing recognition of the gut microbiota as a main partner of human health, we are expecting that the number of indications for fecal microbiota transplantation (FMT) will increase. Thus, there is an urgent need for standardization of the entire process of fecal transplant production. This study provides a complete standardized procedure to prepare and store live and ready-to-use transplants that meet the standard requirements of good practices to applied use in pharmaceutical industry. We show that, if time before transformation to transplants would exceed 24 hours, fresh samples should not be exposed to temperatures above 20 °C, and refrigeration at 4 °C can be a safe solution. Oxygen-free atmosphere was not necessary and simply removing air above collected samples was sufficient to preserve viability. Transplants prepared in maltodextrin-trehalose solutions, stored in a -80 °C standard freezer and then rapidly thawed at 37 °C, retained the best revivification potential as proven by 16S rRNA profiles, metabolomic fingerprints, and flow cytometry assays over a 3-month observation period. Maltodextrin-trehalose containing cryoprotectants were also efficient in preserving viability of lyophilized transplants, either in their crude or purified form, an option that can be attractive for fecal transplant biobanking and oral formulation.Sebastian D. BurzAnne-Laure AbrahamFernanda FonsecaOlivier DavidAudrey ChapronFabienne Béguet-CrespelStéphanie CénardKarine Le RouxOrlane PatrascuFlorence LevenezCarole SchwintnerHervé M. BlottièreChristel Béra-MailletPatricia LepageJoël DoréCatherine JusteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-16 (2019) |
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DOAJ |
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DOAJ |
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| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Sebastian D. Burz Anne-Laure Abraham Fernanda Fonseca Olivier David Audrey Chapron Fabienne Béguet-Crespel Stéphanie Cénard Karine Le Roux Orlane Patrascu Florence Levenez Carole Schwintner Hervé M. Blottière Christel Béra-Maillet Patricia Lepage Joël Doré Catherine Juste A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation |
| description |
Abstract Owing to the growing recognition of the gut microbiota as a main partner of human health, we are expecting that the number of indications for fecal microbiota transplantation (FMT) will increase. Thus, there is an urgent need for standardization of the entire process of fecal transplant production. This study provides a complete standardized procedure to prepare and store live and ready-to-use transplants that meet the standard requirements of good practices to applied use in pharmaceutical industry. We show that, if time before transformation to transplants would exceed 24 hours, fresh samples should not be exposed to temperatures above 20 °C, and refrigeration at 4 °C can be a safe solution. Oxygen-free atmosphere was not necessary and simply removing air above collected samples was sufficient to preserve viability. Transplants prepared in maltodextrin-trehalose solutions, stored in a -80 °C standard freezer and then rapidly thawed at 37 °C, retained the best revivification potential as proven by 16S rRNA profiles, metabolomic fingerprints, and flow cytometry assays over a 3-month observation period. Maltodextrin-trehalose containing cryoprotectants were also efficient in preserving viability of lyophilized transplants, either in their crude or purified form, an option that can be attractive for fecal transplant biobanking and oral formulation. |
| format |
article |
| author |
Sebastian D. Burz Anne-Laure Abraham Fernanda Fonseca Olivier David Audrey Chapron Fabienne Béguet-Crespel Stéphanie Cénard Karine Le Roux Orlane Patrascu Florence Levenez Carole Schwintner Hervé M. Blottière Christel Béra-Maillet Patricia Lepage Joël Doré Catherine Juste |
| author_facet |
Sebastian D. Burz Anne-Laure Abraham Fernanda Fonseca Olivier David Audrey Chapron Fabienne Béguet-Crespel Stéphanie Cénard Karine Le Roux Orlane Patrascu Florence Levenez Carole Schwintner Hervé M. Blottière Christel Béra-Maillet Patricia Lepage Joël Doré Catherine Juste |
| author_sort |
Sebastian D. Burz |
| title |
A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation |
| title_short |
A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation |
| title_full |
A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation |
| title_fullStr |
A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation |
| title_full_unstemmed |
A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation |
| title_sort |
guide for ex vivo handling and storage of stool samples intended for fecal microbiota transplantation |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/bed83d0e2df642338ed18bee829abe8e |
| work_keys_str_mv |
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