Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma

Abstract Purpose Osteosarcoma (OS) is a differentiation disease caused by the genetic and epigenetic differentiation of mesenchymal stem cells into osteoblasts. OS is a common, highly malignant tumor in children and adolescents. Fifteen to 20 % of the patients find distant metastases at their first...

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Autores principales:	Pei Zhang, Keteng Xu, Jingcheng Wang, Jiale Zhang, Huahong Quan
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Osteosarcoma N6-methylandenosine Bioinformatics Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/bed86f2e3fe2499696b6fe3aba5ff352
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spelling	oai:doaj.org-article:bed86f2e3fe2499696b6fe3aba5ff3522021-12-05T12:19:31ZIdentification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma10.1186/s12885-021-09011-z1471-2407https://doaj.org/article/bed86f2e3fe2499696b6fe3aba5ff3522021-12-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-09011-zhttps://doaj.org/toc/1471-2407Abstract Purpose Osteosarcoma (OS) is a differentiation disease caused by the genetic and epigenetic differentiation of mesenchymal stem cells into osteoblasts. OS is a common, highly malignant tumor in children and adolescents. Fifteen to 20 % of the patients find distant metastases at their first visit. The purpose of our study was to identify biomarkers for tracking the prognosis and treatment of OS to improve the survival rate of patients. Materials and methods In this study, which was based on Therapeutically Applicable Research to Generate Effective Treatments (TARGET), we searched for m6A related lncRNAs in OS. We constructed a network between lncRNA and m6A, and built an OS prognostic risk model. Results We identified 14,581 lncRNAs by using the dataset from TARGET. We obtained 111 m6A-related lncRNAs through a Pearson correlation analysis. A network was built between lncRNA and m6A genes. Eight m6A-related lncRNAs associated with survival were identified through a univariate Cox analysis. A selection operator (LASSO) Cox regression was used to construct a prognostic risk model with six genes (RP11-286E11.1, LINC01426, AC010127.3, DLGAP1-AS2, RP4-657D16.3, AC002398.11) obtained through least absolute shrinkage. We also discovered upregulated levels of DLGAP1-AS2 and m6A methylation in osteosarcoma tissues/cells compared with normal tissues/osteoblasts cells. Conclusion We constructed a risk score prognosis model of m6A-related lncRNAs (RP11-286E11.1, LINC01426, AC010127.3, DLGAP1-AS2, RP4-657D16.3, AC002398.11) using the dataset downloaded from TRAGET. We verified the value of the model by dividing all samples into test groups and training groups. However, the role of m6A-related lncRNAs in osteosarcoma needs to be further researched by cell and in vivo studies.Pei ZhangKeteng XuJingcheng WangJiale ZhangHuahong QuanBMCarticleOsteosarcomaN6-methylandenosineBioinformaticsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteosarcoma N6-methylandenosine Bioinformatics Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Osteosarcoma N6-methylandenosine Bioinformatics Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Pei Zhang Keteng Xu Jingcheng Wang Jiale Zhang Huahong Quan Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma
description	Abstract Purpose Osteosarcoma (OS) is a differentiation disease caused by the genetic and epigenetic differentiation of mesenchymal stem cells into osteoblasts. OS is a common, highly malignant tumor in children and adolescents. Fifteen to 20 % of the patients find distant metastases at their first visit. The purpose of our study was to identify biomarkers for tracking the prognosis and treatment of OS to improve the survival rate of patients. Materials and methods In this study, which was based on Therapeutically Applicable Research to Generate Effective Treatments (TARGET), we searched for m6A related lncRNAs in OS. We constructed a network between lncRNA and m6A, and built an OS prognostic risk model. Results We identified 14,581 lncRNAs by using the dataset from TARGET. We obtained 111 m6A-related lncRNAs through a Pearson correlation analysis. A network was built between lncRNA and m6A genes. Eight m6A-related lncRNAs associated with survival were identified through a univariate Cox analysis. A selection operator (LASSO) Cox regression was used to construct a prognostic risk model with six genes (RP11-286E11.1, LINC01426, AC010127.3, DLGAP1-AS2, RP4-657D16.3, AC002398.11) obtained through least absolute shrinkage. We also discovered upregulated levels of DLGAP1-AS2 and m6A methylation in osteosarcoma tissues/cells compared with normal tissues/osteoblasts cells. Conclusion We constructed a risk score prognosis model of m6A-related lncRNAs (RP11-286E11.1, LINC01426, AC010127.3, DLGAP1-AS2, RP4-657D16.3, AC002398.11) using the dataset downloaded from TRAGET. We verified the value of the model by dividing all samples into test groups and training groups. However, the role of m6A-related lncRNAs in osteosarcoma needs to be further researched by cell and in vivo studies.
format	article
author	Pei Zhang Keteng Xu Jingcheng Wang Jiale Zhang Huahong Quan
author_facet	Pei Zhang Keteng Xu Jingcheng Wang Jiale Zhang Huahong Quan
author_sort	Pei Zhang
title	Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma
title_short	Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma
title_full	Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma
title_fullStr	Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma
title_full_unstemmed	Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma
title_sort	identification of n6-methylandenosine related lncrnas biomarkers associated with the overall survival of osteosarcoma
publisher	BMC
publishDate	2021
url	https://doaj.org/article/bed86f2e3fe2499696b6fe3aba5ff352
work_keys_str_mv	AT peizhang identificationofn6methylandenosinerelatedlncrnasbiomarkersassociatedwiththeoverallsurvivalofosteosarcoma AT ketengxu identificationofn6methylandenosinerelatedlncrnasbiomarkersassociatedwiththeoverallsurvivalofosteosarcoma AT jingchengwang identificationofn6methylandenosinerelatedlncrnasbiomarkersassociatedwiththeoverallsurvivalofosteosarcoma AT jialezhang identificationofn6methylandenosinerelatedlncrnasbiomarkersassociatedwiththeoverallsurvivalofosteosarcoma AT huahongquan identificationofn6methylandenosinerelatedlncrnasbiomarkersassociatedwiththeoverallsurvivalofosteosarcoma
_version_	1718372038922469376

Identification of N6-methylandenosine related LncRNAs biomarkers associated with the overall survival of osteosarcoma

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