Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring

Abstract Influenza virus causes life-threatening infections in pregnant women and their newborns. Immunization during pregnancy is the most effective means of preventing maternal and infant mortality/morbidity; however, influenza vaccination rates of pregnant women remain under 50%. Furthermore, the...

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Autores principales:	E. Stein Esser, Joanna A. Pulit-Penaloza, Haripriya Kalluri, Devin McAllister, Elena V. Vassilieva, Elizabeth Q. Littauer, Nadia Lelutiu, Mark R. Prausnitz, Richard W. Compans, Ioanna Skountzou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bee56125b1cf4f40b60481bcbec9c6ab
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spelling	oai:doaj.org-article:bee56125b1cf4f40b60481bcbec9c6ab2021-12-02T16:07:59ZMicroneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring10.1038/s41598-017-05940-72045-2322https://doaj.org/article/bee56125b1cf4f40b60481bcbec9c6ab2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05940-7https://doaj.org/toc/2045-2322Abstract Influenza virus causes life-threatening infections in pregnant women and their newborns. Immunization during pregnancy is the most effective means of preventing maternal and infant mortality/morbidity; however, influenza vaccination rates of pregnant women remain under 50%. Furthermore, the availability of vaccines in low-resource populations is limited. Skin immunization with microneedle patches (MN) is a novel and safe vaccination platform featuring thermostable vaccine formulations. Cold-chain independence and the potential for self-administration can expand influenza vaccination coverage in developing countries. In this study of pregnant BALB/c mice immunized with subunit H1N1 influenza vaccine, we demonstrate the advantage of skin vaccination over intramuscular delivery of a two-fold higher vaccine dose. MN vaccine induced superior humoral immune responses and conferred protective immunity against a lethal challenge dose of homologous influenza virus. Importantly, MN vaccination of mice at mid-gestation resulted in enhanced and long-lasting passive immunity of the offspring, measured by neutralizing antibody titers and survival rates after virus challenge. We conclude that skin vaccination using MN is a superior immunization approach with the potential to overcome immune tolerance observed in pregnancy, and lower vaccination costs through antigen dose-sparing, which is especially relevant in underserved countries.E. Stein EsserJoanna A. Pulit-PenalozaHaripriya KalluriDevin McAllisterElena V. VassilievaElizabeth Q. LittauerNadia LelutiuMark R. PrausnitzRichard W. CompansIoanna SkountzouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E. Stein Esser Joanna A. Pulit-Penaloza Haripriya Kalluri Devin McAllister Elena V. Vassilieva Elizabeth Q. Littauer Nadia Lelutiu Mark R. Prausnitz Richard W. Compans Ioanna Skountzou Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring
description	Abstract Influenza virus causes life-threatening infections in pregnant women and their newborns. Immunization during pregnancy is the most effective means of preventing maternal and infant mortality/morbidity; however, influenza vaccination rates of pregnant women remain under 50%. Furthermore, the availability of vaccines in low-resource populations is limited. Skin immunization with microneedle patches (MN) is a novel and safe vaccination platform featuring thermostable vaccine formulations. Cold-chain independence and the potential for self-administration can expand influenza vaccination coverage in developing countries. In this study of pregnant BALB/c mice immunized with subunit H1N1 influenza vaccine, we demonstrate the advantage of skin vaccination over intramuscular delivery of a two-fold higher vaccine dose. MN vaccine induced superior humoral immune responses and conferred protective immunity against a lethal challenge dose of homologous influenza virus. Importantly, MN vaccination of mice at mid-gestation resulted in enhanced and long-lasting passive immunity of the offspring, measured by neutralizing antibody titers and survival rates after virus challenge. We conclude that skin vaccination using MN is a superior immunization approach with the potential to overcome immune tolerance observed in pregnancy, and lower vaccination costs through antigen dose-sparing, which is especially relevant in underserved countries.
format	article
author	E. Stein Esser Joanna A. Pulit-Penaloza Haripriya Kalluri Devin McAllister Elena V. Vassilieva Elizabeth Q. Littauer Nadia Lelutiu Mark R. Prausnitz Richard W. Compans Ioanna Skountzou
author_facet	E. Stein Esser Joanna A. Pulit-Penaloza Haripriya Kalluri Devin McAllister Elena V. Vassilieva Elizabeth Q. Littauer Nadia Lelutiu Mark R. Prausnitz Richard W. Compans Ioanna Skountzou
author_sort	E. Stein Esser
title	Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring
title_short	Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring
title_full	Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring
title_fullStr	Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring
title_full_unstemmed	Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring
title_sort	microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bee56125b1cf4f40b60481bcbec9c6ab
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Microneedle patch delivery of influenza vaccine during pregnancy enhances maternal immune responses promoting survival and long-lasting passive immunity to offspring

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