DNA methylation profiling in mummified human remains from the eighteenth-century

Abstract Reconstruction of ancient epigenomes by DNA methylation (DNAm) can shed light into the composition of cell types, disease states, and age at death. However, such analysis is hampered by impaired DNA quality and little is known how decomposition affects DNAm. In this study, we determined if...

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Autores principales:	Marco Schmidt, Frank Maixner, Gerhard Hotz, Ildikó Pap, Ildikó Szikossy, György Pálfi, Albert Zink, Wolfgang Wagner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bee5fab012094b68a2b309edebaed130
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spelling	oai:doaj.org-article:bee5fab012094b68a2b309edebaed1302021-12-02T16:31:52ZDNA methylation profiling in mummified human remains from the eighteenth-century10.1038/s41598-021-95021-72045-2322https://doaj.org/article/bee5fab012094b68a2b309edebaed1302021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95021-7https://doaj.org/toc/2045-2322Abstract Reconstruction of ancient epigenomes by DNA methylation (DNAm) can shed light into the composition of cell types, disease states, and age at death. However, such analysis is hampered by impaired DNA quality and little is known how decomposition affects DNAm. In this study, we determined if EPIC Illumina BeadChip technology is applicable for specimens from mummies of the eighteenth century CE. Overall, the signal intensity on the microarray was extremely low, but for one of two samples we were able to detect characteristic DNAm signals in a subset of CG dinucleotides (CpGs), which were selected with a stringent processing pipeline. Using only these CpGs we could train epigenetic signatures with reference DNAm profiles of multiple tissues and our predictions matched the fact that the specimen was lung tissue from a 28-year-old woman. Thus, we provide proof of principle that Illumina BeadChips are applicable for DNAm profiling in ancient samples.Marco SchmidtFrank MaixnerGerhard HotzIldikó PapIldikó SzikossyGyörgy PálfiAlbert ZinkWolfgang WagnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marco Schmidt Frank Maixner Gerhard Hotz Ildikó Pap Ildikó Szikossy György Pálfi Albert Zink Wolfgang Wagner DNA methylation profiling in mummified human remains from the eighteenth-century
description	Abstract Reconstruction of ancient epigenomes by DNA methylation (DNAm) can shed light into the composition of cell types, disease states, and age at death. However, such analysis is hampered by impaired DNA quality and little is known how decomposition affects DNAm. In this study, we determined if EPIC Illumina BeadChip technology is applicable for specimens from mummies of the eighteenth century CE. Overall, the signal intensity on the microarray was extremely low, but for one of two samples we were able to detect characteristic DNAm signals in a subset of CG dinucleotides (CpGs), which were selected with a stringent processing pipeline. Using only these CpGs we could train epigenetic signatures with reference DNAm profiles of multiple tissues and our predictions matched the fact that the specimen was lung tissue from a 28-year-old woman. Thus, we provide proof of principle that Illumina BeadChips are applicable for DNAm profiling in ancient samples.
format	article
author	Marco Schmidt Frank Maixner Gerhard Hotz Ildikó Pap Ildikó Szikossy György Pálfi Albert Zink Wolfgang Wagner
author_facet	Marco Schmidt Frank Maixner Gerhard Hotz Ildikó Pap Ildikó Szikossy György Pálfi Albert Zink Wolfgang Wagner
author_sort	Marco Schmidt
title	DNA methylation profiling in mummified human remains from the eighteenth-century
title_short	DNA methylation profiling in mummified human remains from the eighteenth-century
title_full	DNA methylation profiling in mummified human remains from the eighteenth-century
title_fullStr	DNA methylation profiling in mummified human remains from the eighteenth-century
title_full_unstemmed	DNA methylation profiling in mummified human remains from the eighteenth-century
title_sort	dna methylation profiling in mummified human remains from the eighteenth-century
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bee5fab012094b68a2b309edebaed130
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DNA methylation profiling in mummified human remains from the eighteenth-century

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