Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.

The transcription factor C/EBPβ controls differentiation, proliferation, and functionality of many cell types, including innate immune cells. A detailed molecular understanding of how C/EBPβ directs alternative cell fates remains largely elusive. A multitude of signal-dependent post-translational mo...

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Autores principales:	Bilyana Stoilova, Elisabeth Kowenz-Leutz, Marina Scheller, Achim Leutz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bee7f42a2ec74de386d74419d1727423
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spelling	oai:doaj.org-article:bee7f42a2ec74de386d74419d17274232021-11-18T07:42:57ZLymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.1932-620310.1371/journal.pone.0065169https://doaj.org/article/bee7f42a2ec74de386d74419d17274232013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755188/?tool=EBIhttps://doaj.org/toc/1932-6203The transcription factor C/EBPβ controls differentiation, proliferation, and functionality of many cell types, including innate immune cells. A detailed molecular understanding of how C/EBPβ directs alternative cell fates remains largely elusive. A multitude of signal-dependent post-translational modifications (PTMs) differentially affect the protean C/EBPβ functions. In this study we apply an assay that converts primary mouse B lymphoid progenitors into myeloid cells in order to answer the question how C/EBPβ regulates (trans-) differentiation and determines myeloid cell fate. We found that structural alterations and various C/EBPβ PTMs determine the outcome of trans-differentiation of lymphoid into myeloid cells, including different types of monocytes/macrophages, dendritic cells, and granulocytes. The ability of C/EBPβ to recruit chromatin remodeling complexes is required for the granulocytic trans-differentiation outcome. These novel findings reveal that PTMs and structural plasticity of C/EBPβ are adaptable modular properties that integrate and rewire epigenetic functions to direct differentiation to diverse innate immune system cells, which are crucial for the organism survival.Bilyana StoilovaElisabeth Kowenz-LeutzMarina SchellerAchim LeutzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65169 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bilyana Stoilova Elisabeth Kowenz-Leutz Marina Scheller Achim Leutz Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.
description	The transcription factor C/EBPβ controls differentiation, proliferation, and functionality of many cell types, including innate immune cells. A detailed molecular understanding of how C/EBPβ directs alternative cell fates remains largely elusive. A multitude of signal-dependent post-translational modifications (PTMs) differentially affect the protean C/EBPβ functions. In this study we apply an assay that converts primary mouse B lymphoid progenitors into myeloid cells in order to answer the question how C/EBPβ regulates (trans-) differentiation and determines myeloid cell fate. We found that structural alterations and various C/EBPβ PTMs determine the outcome of trans-differentiation of lymphoid into myeloid cells, including different types of monocytes/macrophages, dendritic cells, and granulocytes. The ability of C/EBPβ to recruit chromatin remodeling complexes is required for the granulocytic trans-differentiation outcome. These novel findings reveal that PTMs and structural plasticity of C/EBPβ are adaptable modular properties that integrate and rewire epigenetic functions to direct differentiation to diverse innate immune system cells, which are crucial for the organism survival.
format	article
author	Bilyana Stoilova Elisabeth Kowenz-Leutz Marina Scheller Achim Leutz
author_facet	Bilyana Stoilova Elisabeth Kowenz-Leutz Marina Scheller Achim Leutz
author_sort	Bilyana Stoilova
title	Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.
title_short	Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.
title_full	Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.
title_fullStr	Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.
title_full_unstemmed	Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.
title_sort	lymphoid to myeloid cell trans-differentiation is determined by c/ebpβ structure and post-translational modifications.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bee7f42a2ec74de386d74419d1727423
work_keys_str_mv	AT bilyanastoilova lymphoidtomyeloidcelltransdifferentiationisdeterminedbycebpbstructureandposttranslationalmodifications AT elisabethkowenzleutz lymphoidtomyeloidcelltransdifferentiationisdeterminedbycebpbstructureandposttranslationalmodifications AT marinascheller lymphoidtomyeloidcelltransdifferentiationisdeterminedbycebpbstructureandposttranslationalmodifications AT achimleutz lymphoidtomyeloidcelltransdifferentiationisdeterminedbycebpbstructureandposttranslationalmodifications
_version_	1718423059788988416

Lymphoid to myeloid cell trans-differentiation is determined by C/EBPβ structure and post-translational modifications.

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