Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy

Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy

Abstract Fluorescence endomicroscopy provides quick access to molecular targets, while Raman spectroscopy allows the detection of multiple molecular targets. Using a simultaneous fluorescence-Raman endoscopic system (FRES), we herein demonstrate its potential in cancer diagnosis in an orthotopically...

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Autores principales: Yong-il Kim, Sinyoung Jeong, Kyung Oh Jung, Myung Geun Song, Chul-Hee Lee, Seock-jin Chung, Ji Yong Park, Myeong Geun Cha, Sung Gun Lee, Bong-Hyun Jun, Yun-Sang Lee, Do Won Hwang, Hyewon Youn, Keon Wook Kang, Yoon-Sik Lee, Dae Hong Jeong, Dong Soo Lee
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/bef0325cb36b406db42e10c272d0faa9
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spelling oai:doaj.org-article:bef0325cb36b406db42e10c272d0faa92021-12-02T12:30:17ZSimultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy10.1038/s41598-017-01020-y2045-2322https://doaj.org/article/bef0325cb36b406db42e10c272d0faa92017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01020-yhttps://doaj.org/toc/2045-2322Abstract Fluorescence endomicroscopy provides quick access to molecular targets, while Raman spectroscopy allows the detection of multiple molecular targets. Using a simultaneous fluorescence-Raman endoscopic system (FRES), we herein demonstrate its potential in cancer diagnosis in an orthotopically induced colorectal cancer (CRC) xenograft model. In the model, epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) were targeted with antibody-conjugated fluorescence and surface-enhanced Raman scattering (F-SERS) dots. FRES demonstrated fast signal detection and multiplex targeting ability using fluorescence and Raman signals to detect the F-SERS dots. In addition, FRES showed a multiplex targeting ability even on a subcentimeter-sized CRC after spraying with a dose of 50 µg F-SERS dots. In conclusion, molecular characteristics of tumor cells (EGFR in cancer cell membranes) and tumor microenvironments (VEGF in the extracellular matrix) could be simultaneously investigated when performing a colonoscopy.Yong-il KimSinyoung JeongKyung Oh JungMyung Geun SongChul-Hee LeeSeock-jin ChungJi Yong ParkMyeong Geun ChaSung Gun LeeBong-Hyun JunYun-Sang LeeDo Won HwangHyewon YounKeon Wook KangYoon-Sik LeeDae Hong JeongDong Soo LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yong-il Kim
Sinyoung Jeong
Kyung Oh Jung
Myung Geun Song
Chul-Hee Lee
Seock-jin Chung
Ji Yong Park
Myeong Geun Cha
Sung Gun Lee
Bong-Hyun Jun
Yun-Sang Lee
Do Won Hwang
Hyewon Youn
Keon Wook Kang
Yoon-Sik Lee
Dae Hong Jeong
Dong Soo Lee
Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy
description Abstract Fluorescence endomicroscopy provides quick access to molecular targets, while Raman spectroscopy allows the detection of multiple molecular targets. Using a simultaneous fluorescence-Raman endoscopic system (FRES), we herein demonstrate its potential in cancer diagnosis in an orthotopically induced colorectal cancer (CRC) xenograft model. In the model, epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) were targeted with antibody-conjugated fluorescence and surface-enhanced Raman scattering (F-SERS) dots. FRES demonstrated fast signal detection and multiplex targeting ability using fluorescence and Raman signals to detect the F-SERS dots. In addition, FRES showed a multiplex targeting ability even on a subcentimeter-sized CRC after spraying with a dose of 50 µg F-SERS dots. In conclusion, molecular characteristics of tumor cells (EGFR in cancer cell membranes) and tumor microenvironments (VEGF in the extracellular matrix) could be simultaneously investigated when performing a colonoscopy.
format article
author Yong-il Kim
Sinyoung Jeong
Kyung Oh Jung
Myung Geun Song
Chul-Hee Lee
Seock-jin Chung
Ji Yong Park
Myeong Geun Cha
Sung Gun Lee
Bong-Hyun Jun
Yun-Sang Lee
Do Won Hwang
Hyewon Youn
Keon Wook Kang
Yoon-Sik Lee
Dae Hong Jeong
Dong Soo Lee
author_facet Yong-il Kim
Sinyoung Jeong
Kyung Oh Jung
Myung Geun Song
Chul-Hee Lee
Seock-jin Chung
Ji Yong Park
Myeong Geun Cha
Sung Gun Lee
Bong-Hyun Jun
Yun-Sang Lee
Do Won Hwang
Hyewon Youn
Keon Wook Kang
Yoon-Sik Lee
Dae Hong Jeong
Dong Soo Lee
author_sort Yong-il Kim
title Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy
title_short Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy
title_full Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy
title_fullStr Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy
title_full_unstemmed Simultaneous Detection of EGFR and VEGF in Colorectal Cancer using Fluorescence-Raman Endoscopy
title_sort simultaneous detection of egfr and vegf in colorectal cancer using fluorescence-raman endoscopy
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/bef0325cb36b406db42e10c272d0faa9
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