Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042

ABSTRACT Bacterial genomes sometimes contain genes that code for homologues of global regulators, the function of which is unclear. In members of the family Enterobacteriaceae, cells express the global regulator H-NS and its paralogue StpA. In Escherichia coli, out of providing a molecular backup fo...

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Autores principales:	A. Prieto, M. Bernabeu, S. Aznar, S. Ruiz-Cruz, A. Bravo, M. H. Queiroz, A. Juárez
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	EAEC H-NS gene regulation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bef27f62d8bf495abb9cd4c0279f448b
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spelling	oai:doaj.org-article:bef27f62d8bf495abb9cd4c0279f448b2021-12-02T18:39:16ZEvolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 04210.1128/mSystems.00220-172379-5077https://doaj.org/article/bef27f62d8bf495abb9cd4c0279f448b2018-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00220-17https://doaj.org/toc/2379-5077ABSTRACT Bacterial genomes sometimes contain genes that code for homologues of global regulators, the function of which is unclear. In members of the family Enterobacteriaceae, cells express the global regulator H-NS and its paralogue StpA. In Escherichia coli, out of providing a molecular backup for H-NS, the role of StpA is poorly characterized. The enteroaggregative E. coli strain 042 carries, in addition to the hns and stpA genes, a third gene encoding an hns paralogue (hns2). We present in this paper information about its biological function. Transcriptomic analysis has shown that the H-NS2 protein targets a subset of the genes targeted by H-NS. Genes targeted by H-NS2 correspond mainly with horizontally transferred (HGT) genes and are also targeted by the Hha protein, a fine-tuner of H-NS activity. Compared with H-NS, H-NS2 expression levels are lower. In addition, H-NS2 expression exhibits specific features: it is sensitive to the growth temperature and to the nature of the culture medium. This novel H-NS paralogue is widespread within the Enterobacteriaceae. IMPORTANCE Global regulators such as H-NS play key relevant roles enabling bacterial cells to adapt to a changing environment. H-NS modulates both core and horizontally transferred (HGT) genes, but the mechanism by which H-NS can differentially regulate these genes remains to be elucidated. There are several instances of bacterial cells carrying genes that encode homologues of the global regulators. The question is what the roles of these proteins are. We noticed that the enteroaggregative E. coli strain 042 carries a new hitherto uncharacterized copy of the hns gene. We decided to investigate why this pathogenic E. coli strain requires an extra H-NS paralogue, termed H-NS2. In our work, we show that H-NS2 displays specific expression and regulatory properties. H-NS2 targets a subset of H-NS-specific genes and may help to differentially modulate core and HGT genes by the H-NS cellular pool.A. PrietoM. BernabeuS. AznarS. Ruiz-CruzA. BravoM. H. QueirozA. JuárezAmerican Society for MicrobiologyarticleEAECH-NSgene regulationMicrobiologyQR1-502ENmSystems, Vol 3, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	EAEC H-NS gene regulation Microbiology QR1-502
spellingShingle	EAEC H-NS gene regulation Microbiology QR1-502 A. Prieto M. Bernabeu S. Aznar S. Ruiz-Cruz A. Bravo M. H. Queiroz A. Juárez Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042
description	ABSTRACT Bacterial genomes sometimes contain genes that code for homologues of global regulators, the function of which is unclear. In members of the family Enterobacteriaceae, cells express the global regulator H-NS and its paralogue StpA. In Escherichia coli, out of providing a molecular backup for H-NS, the role of StpA is poorly characterized. The enteroaggregative E. coli strain 042 carries, in addition to the hns and stpA genes, a third gene encoding an hns paralogue (hns2). We present in this paper information about its biological function. Transcriptomic analysis has shown that the H-NS2 protein targets a subset of the genes targeted by H-NS. Genes targeted by H-NS2 correspond mainly with horizontally transferred (HGT) genes and are also targeted by the Hha protein, a fine-tuner of H-NS activity. Compared with H-NS, H-NS2 expression levels are lower. In addition, H-NS2 expression exhibits specific features: it is sensitive to the growth temperature and to the nature of the culture medium. This novel H-NS paralogue is widespread within the Enterobacteriaceae. IMPORTANCE Global regulators such as H-NS play key relevant roles enabling bacterial cells to adapt to a changing environment. H-NS modulates both core and horizontally transferred (HGT) genes, but the mechanism by which H-NS can differentially regulate these genes remains to be elucidated. There are several instances of bacterial cells carrying genes that encode homologues of the global regulators. The question is what the roles of these proteins are. We noticed that the enteroaggregative E. coli strain 042 carries a new hitherto uncharacterized copy of the hns gene. We decided to investigate why this pathogenic E. coli strain requires an extra H-NS paralogue, termed H-NS2. In our work, we show that H-NS2 displays specific expression and regulatory properties. H-NS2 targets a subset of H-NS-specific genes and may help to differentially modulate core and HGT genes by the H-NS cellular pool.
format	article
author	A. Prieto M. Bernabeu S. Aznar S. Ruiz-Cruz A. Bravo M. H. Queiroz A. Juárez
author_facet	A. Prieto M. Bernabeu S. Aznar S. Ruiz-Cruz A. Bravo M. H. Queiroz A. Juárez
author_sort	A. Prieto
title	Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042
title_short	Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042
title_full	Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042
title_fullStr	Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042
title_full_unstemmed	Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042
title_sort	evolution of bacterial global modulators: role of a novel h-ns paralogue in the enteroaggregative <italic toggle="yes">escherichia coli</italic> strain 042
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/bef27f62d8bf495abb9cd4c0279f448b
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Evolution of Bacterial Global Modulators: Role of a Novel H-NS Paralogue in the Enteroaggregative <italic toggle="yes">Escherichia coli</italic> Strain 042

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