The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers

Cells interact with 3D fibrous platform topography via a nano-scaled focal adhesion complex, and more research is required on how osteoblasts sense and respond to random and aligned fibers through nano-sized focal adhesions and their downstream events. The present study assessed human primary osteob...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pingping Han, Cedryck Vaquette, Abdalla Abdal-hay, Sašo Ivanovski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microgeometry mechanobiology global DNA methylation osteoblast mechanosensing Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bef815d8f6a64e55a1578cb68edb6e88
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bef815d8f6a64e55a1578cb68edb6e88
record_format	dspace
spelling	oai:doaj.org-article:bef815d8f6a64e55a1578cb68edb6e882021-11-25T18:31:02ZThe Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers10.3390/nano111129432079-4991https://doaj.org/article/bef815d8f6a64e55a1578cb68edb6e882021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2943https://doaj.org/toc/2079-4991Cells interact with 3D fibrous platform topography via a nano-scaled focal adhesion complex, and more research is required on how osteoblasts sense and respond to random and aligned fibers through nano-sized focal adhesions and their downstream events. The present study assessed human primary osteoblast cells’ sensing and response to random and aligned medical-grade polycaprolactone (PCL) fibrous 3D scaffolds fabricated via the melt electrowriting (MEW) technique. Cells cultured on a tissue culture plate (TCP) were used as 2D controls. Compared to 2D TCP, 3D MEW fibrous substrates led to immature vinculin focal adhesion formation and significantly reduced nuclear localization of the mechanosensor-yes-associated protein (YAP). Notably, aligned MEW fibers induced elongated cell and nucleus shape and highly activated global DNA methylation of 5-methylcytosine, 5-hydroxymethylcytosine, and N-6 methylated deoxyadenosine compared to the random fibers. Furthermore, although osteogenic markers (<i>osterix-OSX</i> and <i>bone sialoprotein-BSP</i>) were significantly enhanced in PCL-R and PCL-A groups at seven days post-osteogenic differentiation, calcium deposits on all seeded samples did not show a difference after normalizing for DNA content after three weeks of osteogenic induction. Overall, our study linked 3D extracellular fiber alignment to nano-focal adhesion complex, nuclear mechanosensing, DNA epigenetics at an early point (24 h), and longer-term changes in osteoblast osteogenic differentiation.Pingping HanCedryck VaquetteAbdalla Abdal-haySašo IvanovskiMDPI AGarticlemicrogeometrymechanobiologyglobal DNA methylationosteoblast mechanosensingChemistryQD1-999ENNanomaterials, Vol 11, Iss 2943, p 2943 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microgeometry mechanobiology global DNA methylation osteoblast mechanosensing Chemistry QD1-999
spellingShingle	microgeometry mechanobiology global DNA methylation osteoblast mechanosensing Chemistry QD1-999 Pingping Han Cedryck Vaquette Abdalla Abdal-hay Sašo Ivanovski The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers
description	Cells interact with 3D fibrous platform topography via a nano-scaled focal adhesion complex, and more research is required on how osteoblasts sense and respond to random and aligned fibers through nano-sized focal adhesions and their downstream events. The present study assessed human primary osteoblast cells’ sensing and response to random and aligned medical-grade polycaprolactone (PCL) fibrous 3D scaffolds fabricated via the melt electrowriting (MEW) technique. Cells cultured on a tissue culture plate (TCP) were used as 2D controls. Compared to 2D TCP, 3D MEW fibrous substrates led to immature vinculin focal adhesion formation and significantly reduced nuclear localization of the mechanosensor-yes-associated protein (YAP). Notably, aligned MEW fibers induced elongated cell and nucleus shape and highly activated global DNA methylation of 5-methylcytosine, 5-hydroxymethylcytosine, and N-6 methylated deoxyadenosine compared to the random fibers. Furthermore, although osteogenic markers (<i>osterix-OSX</i> and <i>bone sialoprotein-BSP</i>) were significantly enhanced in PCL-R and PCL-A groups at seven days post-osteogenic differentiation, calcium deposits on all seeded samples did not show a difference after normalizing for DNA content after three weeks of osteogenic induction. Overall, our study linked 3D extracellular fiber alignment to nano-focal adhesion complex, nuclear mechanosensing, DNA epigenetics at an early point (24 h), and longer-term changes in osteoblast osteogenic differentiation.
format	article
author	Pingping Han Cedryck Vaquette Abdalla Abdal-hay Sašo Ivanovski
author_facet	Pingping Han Cedryck Vaquette Abdalla Abdal-hay Sašo Ivanovski
author_sort	Pingping Han
title	The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers
title_short	The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers
title_full	The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers
title_fullStr	The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers
title_full_unstemmed	The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers
title_sort	mechanosensing and global dna methylation of human osteoblasts on mew fibers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bef815d8f6a64e55a1578cb68edb6e88
work_keys_str_mv	AT pingpinghan themechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers AT cedryckvaquette themechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers AT abdallaabdalhay themechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers AT sasoivanovski themechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers AT pingpinghan mechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers AT cedryckvaquette mechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers AT abdallaabdalhay mechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers AT sasoivanovski mechanosensingandglobaldnamethylationofhumanosteoblastsonmewfibers
_version_	1718411040687915008

The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers

Ejemplares similares