Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells

Abstract Transfusion of packed red blood cells (PRBC) to patients in critical states is often accompanied by post-transfusion complications. This may be related with disturbance of properties of PRBC and their membranes during long-term storage in the hemopreservative solution. The purpose of our wo...

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Autores principales:	E. Kozlova, A. Chernysh, V. Moroz, V. Sergunova, O. Gudkova, E. Manchenko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf0b7b2d9d4b44c29376b58b72ae3b50
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spelling	oai:doaj.org-article:bf0b7b2d9d4b44c29376b58b72ae3b502021-12-02T12:30:46ZMorphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells10.1038/s41598-017-08255-92045-2322https://doaj.org/article/bf0b7b2d9d4b44c29376b58b72ae3b502017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08255-9https://doaj.org/toc/2045-2322Abstract Transfusion of packed red blood cells (PRBC) to patients in critical states is often accompanied by post-transfusion complications. This may be related with disturbance of properties of PRBC and their membranes during long-term storage in the hemopreservative solution. The purpose of our work is the study of transformation of morphology, membranes stiffness and nanostructure for assessment of PRBC quality, in vitro. By atomic force microscopy we studied the transformation of cell morphology, the appearance of topological nanodefects of membranes and by atomic force spectroscopy studied the change of membrane stiffness during 40 days of storage of PRBC. It was shown that there is a transition period (20–26 days), in which we observed an increase in the Young’s modulus of the membranes 1.6–2 times and transition of cells into irreversible forms. This process was preceded by the appearance of topological nanodefects of membranes. These parameters can be used for quality assessment of PRBC and for improvement of transfusion rules.E. KozlovaA. ChernyshV. MorozV. SergunovaO. GudkovaE. ManchenkoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E. Kozlova A. Chernysh V. Moroz V. Sergunova O. Gudkova E. Manchenko Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells
description	Abstract Transfusion of packed red blood cells (PRBC) to patients in critical states is often accompanied by post-transfusion complications. This may be related with disturbance of properties of PRBC and their membranes during long-term storage in the hemopreservative solution. The purpose of our work is the study of transformation of morphology, membranes stiffness and nanostructure for assessment of PRBC quality, in vitro. By atomic force microscopy we studied the transformation of cell morphology, the appearance of topological nanodefects of membranes and by atomic force spectroscopy studied the change of membrane stiffness during 40 days of storage of PRBC. It was shown that there is a transition period (20–26 days), in which we observed an increase in the Young’s modulus of the membranes 1.6–2 times and transition of cells into irreversible forms. This process was preceded by the appearance of topological nanodefects of membranes. These parameters can be used for quality assessment of PRBC and for improvement of transfusion rules.
format	article
author	E. Kozlova A. Chernysh V. Moroz V. Sergunova O. Gudkova E. Manchenko
author_facet	E. Kozlova A. Chernysh V. Moroz V. Sergunova O. Gudkova E. Manchenko
author_sort	E. Kozlova
title	Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells
title_short	Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells
title_full	Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells
title_fullStr	Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells
title_full_unstemmed	Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells
title_sort	morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bf0b7b2d9d4b44c29376b58b72ae3b50
work_keys_str_mv	AT ekozlova morphologymembranenanostructureandstiffnessforqualityassessmentofpackedredbloodcells AT achernysh morphologymembranenanostructureandstiffnessforqualityassessmentofpackedredbloodcells AT vmoroz morphologymembranenanostructureandstiffnessforqualityassessmentofpackedredbloodcells AT vsergunova morphologymembranenanostructureandstiffnessforqualityassessmentofpackedredbloodcells AT ogudkova morphologymembranenanostructureandstiffnessforqualityassessmentofpackedredbloodcells AT emanchenko morphologymembranenanostructureandstiffnessforqualityassessmentofpackedredbloodcells
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Morphology, membrane nanostructure and stiffness for quality assessment of packed red blood cells

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