Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin

Abstract Clustered protocadherins (Pcdhs), which are cell adhesion molecules, play a fundamental role in self-recognition and non-self-discrimination by conferring diversity on the cell surface. Although systematic cell-based aggregation assays provide information regarding the binding properties of...

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Autores principales:	Takashi Kanadome, Natsumi Hoshino, Takeharu Nagai, Tomoki Matsuda, Takeshi Yagi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf23360214d64c29a1bee326da08e6f8
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spelling	oai:doaj.org-article:bf23360214d64c29a1bee326da08e6f82021-11-21T12:16:49ZDevelopment of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin10.1038/s41598-021-01481-22045-2322https://doaj.org/article/bf23360214d64c29a1bee326da08e6f82021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01481-2https://doaj.org/toc/2045-2322Abstract Clustered protocadherins (Pcdhs), which are cell adhesion molecules, play a fundamental role in self-recognition and non-self-discrimination by conferring diversity on the cell surface. Although systematic cell-based aggregation assays provide information regarding the binding properties of Pcdhs, direct visualization of Pcdh trans interactions across cells remains challenging. Here, we present Förster resonance energy transfer (FRET)-based indicators for directly visualizing Pcdh trans interactions. We developed the indicators by individually inserting FRET donor and acceptor fluorescent proteins (FPs) into the ectodomain of Pcdh molecules. They enabled successful visualization of specific trans interactions of Pcdh and revealed that the Pcdh trans interaction is highly sensitive to changes in extracellular Ca2+ levels. We expect that FRET-based indicators for visualizing Pcdh trans interactions will provide a new approach for investigating the roles of Pcdh in self-recognition and non-self-discrimination processes.Takashi KanadomeNatsumi HoshinoTakeharu NagaiTomoki MatsudaTakeshi YagiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takashi Kanadome Natsumi Hoshino Takeharu Nagai Tomoki Matsuda Takeshi Yagi Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
description	Abstract Clustered protocadherins (Pcdhs), which are cell adhesion molecules, play a fundamental role in self-recognition and non-self-discrimination by conferring diversity on the cell surface. Although systematic cell-based aggregation assays provide information regarding the binding properties of Pcdhs, direct visualization of Pcdh trans interactions across cells remains challenging. Here, we present Förster resonance energy transfer (FRET)-based indicators for directly visualizing Pcdh trans interactions. We developed the indicators by individually inserting FRET donor and acceptor fluorescent proteins (FPs) into the ectodomain of Pcdh molecules. They enabled successful visualization of specific trans interactions of Pcdh and revealed that the Pcdh trans interaction is highly sensitive to changes in extracellular Ca2+ levels. We expect that FRET-based indicators for visualizing Pcdh trans interactions will provide a new approach for investigating the roles of Pcdh in self-recognition and non-self-discrimination processes.
format	article
author	Takashi Kanadome Natsumi Hoshino Takeharu Nagai Tomoki Matsuda Takeshi Yagi
author_facet	Takashi Kanadome Natsumi Hoshino Takeharu Nagai Tomoki Matsuda Takeshi Yagi
author_sort	Takashi Kanadome
title	Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
title_short	Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
title_full	Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
title_fullStr	Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
title_full_unstemmed	Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
title_sort	development of fret-based indicators for visualizing homophilic trans interaction of a clustered protocadherin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bf23360214d64c29a1bee326da08e6f8
work_keys_str_mv	AT takashikanadome developmentoffretbasedindicatorsforvisualizinghomophilictransinteractionofaclusteredprotocadherin AT natsumihoshino developmentoffretbasedindicatorsforvisualizinghomophilictransinteractionofaclusteredprotocadherin AT takeharunagai developmentoffretbasedindicatorsforvisualizinghomophilictransinteractionofaclusteredprotocadherin AT tomokimatsuda developmentoffretbasedindicatorsforvisualizinghomophilictransinteractionofaclusteredprotocadherin AT takeshiyagi developmentoffretbasedindicatorsforvisualizinghomophilictransinteractionofaclusteredprotocadherin
_version_	1718419091232915456

Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin

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