An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.

Notch family members were first identified as cell adhesion molecules by cell aggregation assays in Drosophila studies. However, they are generally recognized as signaling molecules, and it was unclear if their adhesion function was restricted to Drosophila. We previously demonstrated that a mouse N...

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Autores principales:	Akihiko Murata, Miya Yoshino, Mari Hikosaka, Kazuki Okuyama, Lan Zhou, Seiji Sakano, Hideo Yagita, Shin-Ichi Hayashi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf2a0d7dc70c429794fb611410d4672f
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spelling	oai:doaj.org-article:bf2a0d7dc70c429794fb611410d4672f2021-11-25T05:59:16ZAn evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.1932-620310.1371/journal.pone.0108535https://doaj.org/article/bf2a0d7dc70c429794fb611410d4672f2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108535https://doaj.org/toc/1932-6203Notch family members were first identified as cell adhesion molecules by cell aggregation assays in Drosophila studies. However, they are generally recognized as signaling molecules, and it was unclear if their adhesion function was restricted to Drosophila. We previously demonstrated that a mouse Notch ligand, Delta-like 1 (Dll1) functioned as a cell adhesion molecule. We here investigated whether this adhesion function was conserved in the diversified mammalian Notch ligands consisted of two families, Delta-like (Dll1, Dll3 and Dll4) and Jagged (Jag1 and Jag2). The forced expression of mouse Dll1, Dll4, Jag1, and Jag2, but not Dll3, on stromal cells induced the rapid and enhanced adhesion of cultured mast cells (MCs). This was attributed to the binding of Notch1 and Notch2 on MCs to each Notch ligand on the stromal cells themselves, and not the activation of Notch signaling. Notch receptor-ligand binding strongly supported the tethering of MCs to stromal cells, the first step of cell adhesion. However, the Jag2-mediated adhesion of MCs was weaker and unlike other ligands appeared to require additional factor(s) in addition to the receptor-ligand binding. Taken together, these results demonstrated that the function of cell adhesion was conserved in mammalian as well as Drosophila Notch family members. Since Notch receptor-ligand interaction plays important roles in a broad spectrum of biological processes ranging from embryogenesis to disorders, our finding will provide a new perspective on these issues from the aspect of cell adhesion.Akihiko MurataMiya YoshinoMari HikosakaKazuki OkuyamaLan ZhouSeiji SakanoHideo YagitaShin-Ichi HayashiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108535 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Akihiko Murata Miya Yoshino Mari Hikosaka Kazuki Okuyama Lan Zhou Seiji Sakano Hideo Yagita Shin-Ichi Hayashi An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.
description	Notch family members were first identified as cell adhesion molecules by cell aggregation assays in Drosophila studies. However, they are generally recognized as signaling molecules, and it was unclear if their adhesion function was restricted to Drosophila. We previously demonstrated that a mouse Notch ligand, Delta-like 1 (Dll1) functioned as a cell adhesion molecule. We here investigated whether this adhesion function was conserved in the diversified mammalian Notch ligands consisted of two families, Delta-like (Dll1, Dll3 and Dll4) and Jagged (Jag1 and Jag2). The forced expression of mouse Dll1, Dll4, Jag1, and Jag2, but not Dll3, on stromal cells induced the rapid and enhanced adhesion of cultured mast cells (MCs). This was attributed to the binding of Notch1 and Notch2 on MCs to each Notch ligand on the stromal cells themselves, and not the activation of Notch signaling. Notch receptor-ligand binding strongly supported the tethering of MCs to stromal cells, the first step of cell adhesion. However, the Jag2-mediated adhesion of MCs was weaker and unlike other ligands appeared to require additional factor(s) in addition to the receptor-ligand binding. Taken together, these results demonstrated that the function of cell adhesion was conserved in mammalian as well as Drosophila Notch family members. Since Notch receptor-ligand interaction plays important roles in a broad spectrum of biological processes ranging from embryogenesis to disorders, our finding will provide a new perspective on these issues from the aspect of cell adhesion.
format	article
author	Akihiko Murata Miya Yoshino Mari Hikosaka Kazuki Okuyama Lan Zhou Seiji Sakano Hideo Yagita Shin-Ichi Hayashi
author_facet	Akihiko Murata Miya Yoshino Mari Hikosaka Kazuki Okuyama Lan Zhou Seiji Sakano Hideo Yagita Shin-Ichi Hayashi
author_sort	Akihiko Murata
title	An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.
title_short	An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.
title_full	An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.
title_fullStr	An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.
title_full_unstemmed	An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.
title_sort	evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bf2a0d7dc70c429794fb611410d4672f
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An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.

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