<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production

ABSTRACT Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that can cause severe opportunistic infections. The principal redox buffer employed by this organism is glutathione (GSH). To assess the role of GSH in the virulence of P. aeruginosa, a number of analyses were performed using a...

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Autores principales:	Tricia A. Van Laar, Saika Esani, Tyler J. Birges, Bethany Hazen, Jason M. Thomas, Mamta Rawat
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Pseudomonas aeruginosa biofilms glutathione pyocyanin thiols virulence Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bf3d9f21480d4fbc95ce28d50370df36
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spelling	oai:doaj.org-article:bf3d9f21480d4fbc95ce28d50370df362021-11-15T15:22:13Z<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production10.1128/mSphere.00155-182379-5042https://doaj.org/article/bf3d9f21480d4fbc95ce28d50370df362018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00155-18https://doaj.org/toc/2379-5042ABSTRACT Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that can cause severe opportunistic infections. The principal redox buffer employed by this organism is glutathione (GSH). To assess the role of GSH in the virulence of P. aeruginosa, a number of analyses were performed using a mutant strain deficient in gshA, which does not produce GSH. The mutant strain exhibited a growth delay in minimal medium compared to the wild-type strain. Furthermore, the gshA mutant was defective in biofilm and persister cell formation and in swimming and swarming motility and produced reduced levels of pyocyanin, a key virulence factor. Finally, the gshA mutant strain demonstrated increased sensitivity to methyl viologen (a redox cycling agent) as well as the thiol-reactive antibiotics fosfomycin and rifampin. Taken together, these data suggest a key role for GSH in the virulence of P. aeruginosa. IMPORTANCE Pseudomonas aeruginosa is a ubiquitous bacterium that can cause severe opportunistic infections, including many hospital-acquired infections. It is also a major cause of infections in patients with cystic fibrosis. P. aeruginosa is intrinsically resistant to a number of drugs and is capable of forming biofilms that are difficult to eradicate with antibiotics. The number of drug-resistant strains is also increasing, making treatment of P. aeruginosa infections very difficult. Thus, there is an urgent need to understand how P. aeruginosa causes disease in order to find novel ways to treat infections. We show that the principal redox buffer, glutathione (GSH), is involved in intrinsic resistance to the fosfomycin and rifampin antibiotics. We further demonstrate that GSH plays a role in P. aeruginosa disease and infection, since a mutant lacking GSH has less biofilm formation, is less able to swarm, and produces less pyocyanin, a pigment associated with infection.Tricia A. Van LaarSaika EsaniTyler J. BirgesBethany HazenJason M. ThomasMamta RawatAmerican Society for MicrobiologyarticlePseudomonas aeruginosabiofilmsglutathionepyocyaninthiolsvirulenceMicrobiologyQR1-502ENmSphere, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas aeruginosa biofilms glutathione pyocyanin thiols virulence Microbiology QR1-502
spellingShingle	Pseudomonas aeruginosa biofilms glutathione pyocyanin thiols virulence Microbiology QR1-502 Tricia A. Van Laar Saika Esani Tyler J. Birges Bethany Hazen Jason M. Thomas Mamta Rawat <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production
description	ABSTRACT Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that can cause severe opportunistic infections. The principal redox buffer employed by this organism is glutathione (GSH). To assess the role of GSH in the virulence of P. aeruginosa, a number of analyses were performed using a mutant strain deficient in gshA, which does not produce GSH. The mutant strain exhibited a growth delay in minimal medium compared to the wild-type strain. Furthermore, the gshA mutant was defective in biofilm and persister cell formation and in swimming and swarming motility and produced reduced levels of pyocyanin, a key virulence factor. Finally, the gshA mutant strain demonstrated increased sensitivity to methyl viologen (a redox cycling agent) as well as the thiol-reactive antibiotics fosfomycin and rifampin. Taken together, these data suggest a key role for GSH in the virulence of P. aeruginosa. IMPORTANCE Pseudomonas aeruginosa is a ubiquitous bacterium that can cause severe opportunistic infections, including many hospital-acquired infections. It is also a major cause of infections in patients with cystic fibrosis. P. aeruginosa is intrinsically resistant to a number of drugs and is capable of forming biofilms that are difficult to eradicate with antibiotics. The number of drug-resistant strains is also increasing, making treatment of P. aeruginosa infections very difficult. Thus, there is an urgent need to understand how P. aeruginosa causes disease in order to find novel ways to treat infections. We show that the principal redox buffer, glutathione (GSH), is involved in intrinsic resistance to the fosfomycin and rifampin antibiotics. We further demonstrate that GSH plays a role in P. aeruginosa disease and infection, since a mutant lacking GSH has less biofilm formation, is less able to swarm, and produces less pyocyanin, a pigment associated with infection.
format	article
author	Tricia A. Van Laar Saika Esani Tyler J. Birges Bethany Hazen Jason M. Thomas Mamta Rawat
author_facet	Tricia A. Van Laar Saika Esani Tyler J. Birges Bethany Hazen Jason M. Thomas Mamta Rawat
author_sort	Tricia A. Van Laar
title	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production
title_short	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production
title_full	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production
title_fullStr	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production
title_full_unstemmed	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production
title_sort	<named-content content-type="genus-species">pseudomonas aeruginosa</named-content><italic toggle="yes"> gsha</italic> mutant is defective in biofilm formation, swarming, and pyocyanin production
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/bf3d9f21480d4fbc95ce28d50370df36
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<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content><italic toggle="yes"> gshA</italic> Mutant Is Defective in Biofilm Formation, Swarming, and Pyocyanin Production

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