The microbial composition of the initial insult can predict the prognosis of experimental sepsis

Abstract We hypothesized that the composition of sepsis-inducing bacterial flora influences the course of fecal peritonitis in rodents. Saline or fecal suspensions with a standardized dose range of bacterial colony-forming units (CFUs) were injected intraperitoneally into Sprague–Dawley rats. The qu...

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Autores principales:	Szabolcs Péter Tallósy, Marietta Zita Poles, Attila Rutai, Roland Fejes, László Juhász, Katalin Burián, József Sóki, Andrea Szabó, Mihály Boros, József Kaszaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf4f5a35b6834b18b043f2beb5ee9d9e
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spelling	oai:doaj.org-article:bf4f5a35b6834b18b043f2beb5ee9d9e2021-11-28T12:16:03ZThe microbial composition of the initial insult can predict the prognosis of experimental sepsis10.1038/s41598-021-02129-x2045-2322https://doaj.org/article/bf4f5a35b6834b18b043f2beb5ee9d9e2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02129-xhttps://doaj.org/toc/2045-2322Abstract We hypothesized that the composition of sepsis-inducing bacterial flora influences the course of fecal peritonitis in rodents. Saline or fecal suspensions with a standardized dose range of bacterial colony-forming units (CFUs) were injected intraperitoneally into Sprague–Dawley rats. The qualitative composition of the initial inoculum and the ascites was analyzed separately by MALDI-TOF mass spectrometry. Invasive monitoring was conducted in separate anesthetized groups (n = 12–13/group) after 12, 24, 48 and 72 h to determine rat-specific organ failure assessment (ROFA) scores. Death and ROFA scores peaked at 24 h. At this time, 20% mortality occurred in animals receiving a monomicrobial E. coli suspension, and ROFA scores were significantly higher in the monomicrobial subgroup than in the polymicrobial one (median 6.5; 5.0–7.0 and 5.0; 4.75–5.0, respectively). ROFA scores dropped after 48 h, accompanied by a steady decrease in ascites CFUs and a shift towards intra-abdominal monomicrobial E. coli cultures. Furthermore, we found a relationship between ascites CFUs and the evolving change in ROFA scores throughout the study. Hence, quantitatively identical bacterial loads with mono- or polymicrobial dominance lead to a different degree of sepsis severity and divergent outcomes. Initial and intraperitoneal microbiological testing should be used to improve translational research success.Szabolcs Péter TallósyMarietta Zita PolesAttila RutaiRoland FejesLászló JuhászKatalin BuriánJózsef SókiAndrea SzabóMihály BorosJózsef KaszakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Szabolcs Péter Tallósy Marietta Zita Poles Attila Rutai Roland Fejes László Juhász Katalin Burián József Sóki Andrea Szabó Mihály Boros József Kaszaki The microbial composition of the initial insult can predict the prognosis of experimental sepsis
description	Abstract We hypothesized that the composition of sepsis-inducing bacterial flora influences the course of fecal peritonitis in rodents. Saline or fecal suspensions with a standardized dose range of bacterial colony-forming units (CFUs) were injected intraperitoneally into Sprague–Dawley rats. The qualitative composition of the initial inoculum and the ascites was analyzed separately by MALDI-TOF mass spectrometry. Invasive monitoring was conducted in separate anesthetized groups (n = 12–13/group) after 12, 24, 48 and 72 h to determine rat-specific organ failure assessment (ROFA) scores. Death and ROFA scores peaked at 24 h. At this time, 20% mortality occurred in animals receiving a monomicrobial E. coli suspension, and ROFA scores were significantly higher in the monomicrobial subgroup than in the polymicrobial one (median 6.5; 5.0–7.0 and 5.0; 4.75–5.0, respectively). ROFA scores dropped after 48 h, accompanied by a steady decrease in ascites CFUs and a shift towards intra-abdominal monomicrobial E. coli cultures. Furthermore, we found a relationship between ascites CFUs and the evolving change in ROFA scores throughout the study. Hence, quantitatively identical bacterial loads with mono- or polymicrobial dominance lead to a different degree of sepsis severity and divergent outcomes. Initial and intraperitoneal microbiological testing should be used to improve translational research success.
format	article
author	Szabolcs Péter Tallósy Marietta Zita Poles Attila Rutai Roland Fejes László Juhász Katalin Burián József Sóki Andrea Szabó Mihály Boros József Kaszaki
author_facet	Szabolcs Péter Tallósy Marietta Zita Poles Attila Rutai Roland Fejes László Juhász Katalin Burián József Sóki Andrea Szabó Mihály Boros József Kaszaki
author_sort	Szabolcs Péter Tallósy
title	The microbial composition of the initial insult can predict the prognosis of experimental sepsis
title_short	The microbial composition of the initial insult can predict the prognosis of experimental sepsis
title_full	The microbial composition of the initial insult can predict the prognosis of experimental sepsis
title_fullStr	The microbial composition of the initial insult can predict the prognosis of experimental sepsis
title_full_unstemmed	The microbial composition of the initial insult can predict the prognosis of experimental sepsis
title_sort	microbial composition of the initial insult can predict the prognosis of experimental sepsis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bf4f5a35b6834b18b043f2beb5ee9d9e
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The microbial composition of the initial insult can predict the prognosis of experimental sepsis

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