Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development
The trimethylation of histone H3 lysine 27 (H3K27me3) is one of the most important chromatin modifications, which is generally presented as a repressive mark in various biological processes. However, the dynamic and global-scale distribution of H3K27me3 during porcine embryonic muscle development re...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:bf518b07125c4484b79a008e472b09c12021-11-05T08:52:24ZGenome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development2296-634X10.3389/fcell.2021.739321https://doaj.org/article/bf518b07125c4484b79a008e472b09c12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.739321/fullhttps://doaj.org/toc/2296-634XThe trimethylation of histone H3 lysine 27 (H3K27me3) is one of the most important chromatin modifications, which is generally presented as a repressive mark in various biological processes. However, the dynamic and global-scale distribution of H3K27me3 during porcine embryonic muscle development remains unclear. Here, our study provided a comprehensive genome-wide view of H3K27me3 and analyzed the matching transcriptome in the skeletal muscles on days 33, 65, and 90 post-coitus from Duroc fetuses. Transcriptome analysis identified 4,124 differentially expressed genes (DEGs) and revealed the key transcriptional properties in three stages. We found that the global H3K27me3 levels continually increased during embryonic development, and the H3K27me3 level was negatively correlated with gene expression. The loss of H3K27me3 in the promoter was associated with the transcriptional activation of 856 DEGs in various processes, including skeletal muscle development, calcium signaling, and multiple metabolic pathways. We also identified for the first time that H3K27me3 could enrich in the promoter of genes, such as DES, MYL1, TNNC1, and KLF5, to negatively regulate gene expression in porcine satellite cells (PSCs). The loss of H3K27me3 could promote muscle cell differentiation. Taken together, this study provided the first genome-wide landscape of H3K27me3 in porcine embryonic muscle development. It revealed the complex and broad function of H3K27me3 in the regulation of embryonic muscle development from skeletal muscle morphogenesis to myofiber maturation.Baohua TanBaohua TanSheng WangShanshan WangShanshan WangJiekang ZengJiekang ZengLinjun HongLinjun HongZicong LiZicong LiJie YangJie YangGengyuan CaiGengyuan CaiEnqin ZhengEnqin ZhengZhenfang WuZhenfang WuTing GuTing GuFrontiers Media S.A.articleH3K27me3skeletal muscleembryonic developmentpigChIP-seqBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
H3K27me3 skeletal muscle embryonic development pig ChIP-seq Biology (General) QH301-705.5 |
| spellingShingle |
H3K27me3 skeletal muscle embryonic development pig ChIP-seq Biology (General) QH301-705.5 Baohua Tan Baohua Tan Sheng Wang Shanshan Wang Shanshan Wang Jiekang Zeng Jiekang Zeng Linjun Hong Linjun Hong Zicong Li Zicong Li Jie Yang Jie Yang Gengyuan Cai Gengyuan Cai Enqin Zheng Enqin Zheng Zhenfang Wu Zhenfang Wu Ting Gu Ting Gu Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development |
| description |
The trimethylation of histone H3 lysine 27 (H3K27me3) is one of the most important chromatin modifications, which is generally presented as a repressive mark in various biological processes. However, the dynamic and global-scale distribution of H3K27me3 during porcine embryonic muscle development remains unclear. Here, our study provided a comprehensive genome-wide view of H3K27me3 and analyzed the matching transcriptome in the skeletal muscles on days 33, 65, and 90 post-coitus from Duroc fetuses. Transcriptome analysis identified 4,124 differentially expressed genes (DEGs) and revealed the key transcriptional properties in three stages. We found that the global H3K27me3 levels continually increased during embryonic development, and the H3K27me3 level was negatively correlated with gene expression. The loss of H3K27me3 in the promoter was associated with the transcriptional activation of 856 DEGs in various processes, including skeletal muscle development, calcium signaling, and multiple metabolic pathways. We also identified for the first time that H3K27me3 could enrich in the promoter of genes, such as DES, MYL1, TNNC1, and KLF5, to negatively regulate gene expression in porcine satellite cells (PSCs). The loss of H3K27me3 could promote muscle cell differentiation. Taken together, this study provided the first genome-wide landscape of H3K27me3 in porcine embryonic muscle development. It revealed the complex and broad function of H3K27me3 in the regulation of embryonic muscle development from skeletal muscle morphogenesis to myofiber maturation. |
| format |
article |
| author |
Baohua Tan Baohua Tan Sheng Wang Shanshan Wang Shanshan Wang Jiekang Zeng Jiekang Zeng Linjun Hong Linjun Hong Zicong Li Zicong Li Jie Yang Jie Yang Gengyuan Cai Gengyuan Cai Enqin Zheng Enqin Zheng Zhenfang Wu Zhenfang Wu Ting Gu Ting Gu |
| author_facet |
Baohua Tan Baohua Tan Sheng Wang Shanshan Wang Shanshan Wang Jiekang Zeng Jiekang Zeng Linjun Hong Linjun Hong Zicong Li Zicong Li Jie Yang Jie Yang Gengyuan Cai Gengyuan Cai Enqin Zheng Enqin Zheng Zhenfang Wu Zhenfang Wu Ting Gu Ting Gu |
| author_sort |
Baohua Tan |
| title |
Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development |
| title_short |
Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development |
| title_full |
Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development |
| title_fullStr |
Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development |
| title_full_unstemmed |
Genome-Wide Analysis of H3K27me3 in Porcine Embryonic Muscle Development |
| title_sort |
genome-wide analysis of h3k27me3 in porcine embryonic muscle development |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/bf518b07125c4484b79a008e472b09c1 |
| work_keys_str_mv |
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| _version_ |
1718444495680307200 |