Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain

Abstract We report a metabolomic analysis of Streptomyces sp. ID38640, a soil isolate that produces the bacterial RNA polymerase inhibitor pseudouridimycin. The analysis was performed on the wild type, on three newly constructed and seven previously reported mutant strains disabled in different gene...

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Autores principales:	Marianna Iorio, Sahar Davatgarbenam, Stefania Serina, Paolo Criscenzo, Mitja M. Zdouc, Matteo Simone, Sonia I. Maffioli, Richard H. Ebright, Stefano Donadio, Margherita Sosio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf5c6c742b4b4ec79d73fdd145693768
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spelling	oai:doaj.org-article:bf5c6c742b4b4ec79d73fdd1456937682021-12-02T15:52:21ZBlocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain10.1038/s41598-021-84833-22045-2322https://doaj.org/article/bf5c6c742b4b4ec79d73fdd1456937682021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84833-2https://doaj.org/toc/2045-2322Abstract We report a metabolomic analysis of Streptomyces sp. ID38640, a soil isolate that produces the bacterial RNA polymerase inhibitor pseudouridimycin. The analysis was performed on the wild type, on three newly constructed and seven previously reported mutant strains disabled in different genes required for pseudouridimycin biosynthesis. The results indicate that Streptomyces sp. ID38640 is able to produce, in addition to lydicamycins and deferroxiamines, as previously reported, also the lassopeptide ulleungdin, the non-ribosomal peptide antipain and the osmoprotectant ectoine. The corresponding biosynthetic gene clusters were readily identified in the strain genome. We also detected the known compound pyridindolol, for which we propose a previously unreported biosynthetic gene cluster, as well as three families of unknown metabolites. Remarkably, the levels of most metabolites varied strongly in the different mutant strains, an observation that enabled detection of metabolites unnoticed in the wild type. Systematic investigation of the accumulated metabolites in the ten different pum mutants identified shed further light on pseudouridimycin biosynthesis. We also show that several Streptomyces strains, able to produce pseudouridimycin, have distinct genetic relationship and metabolic profile with ID38640.Marianna IorioSahar DavatgarbenamStefania SerinaPaolo CriscenzoMitja M. ZdoucMatteo SimoneSonia I. MaffioliRichard H. EbrightStefano DonadioMargherita SosioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marianna Iorio Sahar Davatgarbenam Stefania Serina Paolo Criscenzo Mitja M. Zdouc Matteo Simone Sonia I. Maffioli Richard H. Ebright Stefano Donadio Margherita Sosio Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain
description	Abstract We report a metabolomic analysis of Streptomyces sp. ID38640, a soil isolate that produces the bacterial RNA polymerase inhibitor pseudouridimycin. The analysis was performed on the wild type, on three newly constructed and seven previously reported mutant strains disabled in different genes required for pseudouridimycin biosynthesis. The results indicate that Streptomyces sp. ID38640 is able to produce, in addition to lydicamycins and deferroxiamines, as previously reported, also the lassopeptide ulleungdin, the non-ribosomal peptide antipain and the osmoprotectant ectoine. The corresponding biosynthetic gene clusters were readily identified in the strain genome. We also detected the known compound pyridindolol, for which we propose a previously unreported biosynthetic gene cluster, as well as three families of unknown metabolites. Remarkably, the levels of most metabolites varied strongly in the different mutant strains, an observation that enabled detection of metabolites unnoticed in the wild type. Systematic investigation of the accumulated metabolites in the ten different pum mutants identified shed further light on pseudouridimycin biosynthesis. We also show that several Streptomyces strains, able to produce pseudouridimycin, have distinct genetic relationship and metabolic profile with ID38640.
format	article
author	Marianna Iorio Sahar Davatgarbenam Stefania Serina Paolo Criscenzo Mitja M. Zdouc Matteo Simone Sonia I. Maffioli Richard H. Ebright Stefano Donadio Margherita Sosio
author_facet	Marianna Iorio Sahar Davatgarbenam Stefania Serina Paolo Criscenzo Mitja M. Zdouc Matteo Simone Sonia I. Maffioli Richard H. Ebright Stefano Donadio Margherita Sosio
author_sort	Marianna Iorio
title	Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain
title_short	Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain
title_full	Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain
title_fullStr	Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain
title_full_unstemmed	Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain
title_sort	blocks in the pseudouridimycin pathway unlock hidden metabolites in the streptomyces producer strain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bf5c6c742b4b4ec79d73fdd145693768
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Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain

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