Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan

Objective: In this study, the antigen of Brucella was prepared. This antigen is applied for detection of Brucellosis through Wright test. Methods: This antigen can be used in every low equipped laboratory for Wright test. After continuous reviewing of Iranian and foreign references, Brucella abortus...

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Autores principales:	M Khajeh Karamoddini, S Fazli Bazzaz
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 1999
Materias:	brucella abortus 19 antigen wright test brucellosis Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bf5e1cd3736544439f126100511aa724
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spelling	oai:doaj.org-article:bf5e1cd3736544439f126100511aa7242021-11-10T09:24:24ZPreparation of Brucella antigen for diagnosis of Brucellosis in Khorasan1561-41072251-7170https://doaj.org/article/bf5e1cd3736544439f126100511aa7241999-07-01T00:00:00Zhttp://jbums.org/article-1-2947-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Objective: In this study, the antigen of Brucella was prepared. This antigen is applied for detection of Brucellosis through Wright test. Methods: This antigen can be used in every low equipped laboratory for Wright test. After continuous reviewing of Iranian and foreign references, Brucella abortus (Strain 19) were cultured and propagated and then, a suspension of bacteria (In normal saline containing phenol 0.5%) was prepared in sterile conditions and centrifuged. We added the solvent to that and standardize it with spectropherometric method in a wave length of 650nm. Findings: In comparison between commercial antigen preparation of Pasteur institute of Tehran and freshly prepared antigens by this method on 500 samples of serum gained from patient suspected to Brucellosis, Wright test was negative for 427 patients, but the rest of the patients (73) detected to be positive both with standard and freshly prepared antigen. These results wee acceptable and unquestionable agglutination was observed. Conclusion: It is important to remind that this antigen can be prepared for daily applications and its preparation possible in any laboratory even with minimum equipment.M Khajeh KaramoddiniS Fazli BazzazBabol University of Medical Sciencesarticlebrucella abortus 19antigenwright testbrucellosisMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 1, Iss 3, Pp 24-28 (1999)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	brucella abortus 19 antigen wright test brucellosis Medicine R Medicine (General) R5-920
spellingShingle	brucella abortus 19 antigen wright test brucellosis Medicine R Medicine (General) R5-920 M Khajeh Karamoddini S Fazli Bazzaz Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan
description	Objective: In this study, the antigen of Brucella was prepared. This antigen is applied for detection of Brucellosis through Wright test. Methods: This antigen can be used in every low equipped laboratory for Wright test. After continuous reviewing of Iranian and foreign references, Brucella abortus (Strain 19) were cultured and propagated and then, a suspension of bacteria (In normal saline containing phenol 0.5%) was prepared in sterile conditions and centrifuged. We added the solvent to that and standardize it with spectropherometric method in a wave length of 650nm. Findings: In comparison between commercial antigen preparation of Pasteur institute of Tehran and freshly prepared antigens by this method on 500 samples of serum gained from patient suspected to Brucellosis, Wright test was negative for 427 patients, but the rest of the patients (73) detected to be positive both with standard and freshly prepared antigen. These results wee acceptable and unquestionable agglutination was observed. Conclusion: It is important to remind that this antigen can be prepared for daily applications and its preparation possible in any laboratory even with minimum equipment.
format	article
author	M Khajeh Karamoddini S Fazli Bazzaz
author_facet	M Khajeh Karamoddini S Fazli Bazzaz
author_sort	M Khajeh Karamoddini
title	Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan
title_short	Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan
title_full	Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan
title_fullStr	Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan
title_full_unstemmed	Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan
title_sort	preparation of brucella antigen for diagnosis of brucellosis in khorasan
publisher	Babol University of Medical Sciences
publishDate	1999
url	https://doaj.org/article/bf5e1cd3736544439f126100511aa724
work_keys_str_mv	AT mkhajehkaramoddini preparationofbrucellaantigenfordiagnosisofbrucellosisinkhorasan AT sfazlibazzaz preparationofbrucellaantigenfordiagnosisofbrucellosisinkhorasan
_version_	1718440132723343360

Preparation of Brucella antigen for diagnosis of Brucellosis in Khorasan

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