In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials

Han Qiao,1 Doerte Luensmann,1 Miriam Heynen,1 Elizabeth Drolle,1 Lakshman N Subbaraman,1 Charles Scales,2 Donald Riederer,2 Zohra Fadli,2 Lyndon Jones1,3 1Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science, University of Waterloo, Waterloo, ON, Canada...

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Autores principales:	Qiao H, Luensmann D, Heynen M, Drolle E, Subbaraman LN, Scales C, Riederer D, Fadli Z, Jones L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	lipid distribution silicone hydrogel contact lenses cholesteryl ester artificial tear solution Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/bf5eef6a6f1645e3906e1ceeb5a9d255
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spelling	oai:doaj.org-article:bf5eef6a6f1645e3906e1ceeb5a9d2552021-12-02T11:20:18ZIn vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials1177-5483https://doaj.org/article/bf5eef6a6f1645e3906e1ceeb5a9d2552020-09-01T00:00:00Zhttps://www.dovepress.com/in-vitro-evaluation-of-the-location-of-cholesteryl-ester-deposits-on-m-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Han Qiao,1 Doerte Luensmann,1 Miriam Heynen,1 Elizabeth Drolle,1 Lakshman N Subbaraman,1 Charles Scales,2 Donald Riederer,2 Zohra Fadli,2 Lyndon Jones1,3 1Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science, University of Waterloo, Waterloo, ON, Canada; 2Johnson & Johnson Vision Care, Inc, Jacksonville, FL, USA; 3Centre for Eye & Vision Research (CEVR), Hong Kong, People’s Republic of ChinaCorrespondence: Doerte Luensmann Email dluensma@uwaterloo.caPurpose: The deposition profile of cholesteryl ester on the surface and throughout the matrix of silicone hydrogel contact lens (CL) materials was determined under conditions that mimic a daily wear regimen.Methods: In this in vitro study, four SiHy CL materials (senofilcon C, lotrafilcon B, comfilcon A and samfilcon A) were incubated in an artificial tear solution (ATS) for up to 30 days. CL incubation was alternated between the ATS (16 hours) and a multipurpose care regimen (8 hours). The ATS included fluorescently tagged cholesteryl ester (5-cholesten-3ß-ol 6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]caproate; CE-NBD) and confocal laser scanning microscopy visualized the distribution of the lipid through the CLs.Results: The distribution of CE-NBD was homogenous from the anterior to posterior surface in senofilcon C and comfilcon A, at all time points. For lotrafilcon B and samfilcon A, CE-NBD localization was heterogeneous, with greater amounts on the surfaces on Day 1 and Day 14 compared to the lens matrix; however, differences in concentration between the surface and bulk diminished by Day 30.Conclusion: The distribution of the non-polar lipid CE-NBD varied with lens material chemistry. While some lens materials deposited the lipid primarily on the surface after 16 hours of exposure, all materials exhibited a homogenous distribution after one month.Keywords: lipid distribution, silicone hydrogel contact lenses, cholesteryl ester, artificial tear solutionQiao HLuensmann DHeynen MDrolle ESubbaraman LNScales CRiederer DFadli ZJones LDove Medical Pressarticlelipid distributionsilicone hydrogel contact lensescholesteryl esterartificial tear solutionOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 14, Pp 2821-2828 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid distribution silicone hydrogel contact lenses cholesteryl ester artificial tear solution Ophthalmology RE1-994
spellingShingle	lipid distribution silicone hydrogel contact lenses cholesteryl ester artificial tear solution Ophthalmology RE1-994 Qiao H Luensmann D Heynen M Drolle E Subbaraman LN Scales C Riederer D Fadli Z Jones L In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials
description	Han Qiao,1 Doerte Luensmann,1 Miriam Heynen,1 Elizabeth Drolle,1 Lakshman N Subbaraman,1 Charles Scales,2 Donald Riederer,2 Zohra Fadli,2 Lyndon Jones1,3 1Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science, University of Waterloo, Waterloo, ON, Canada; 2Johnson & Johnson Vision Care, Inc, Jacksonville, FL, USA; 3Centre for Eye & Vision Research (CEVR), Hong Kong, People’s Republic of ChinaCorrespondence: Doerte Luensmann Email dluensma@uwaterloo.caPurpose: The deposition profile of cholesteryl ester on the surface and throughout the matrix of silicone hydrogel contact lens (CL) materials was determined under conditions that mimic a daily wear regimen.Methods: In this in vitro study, four SiHy CL materials (senofilcon C, lotrafilcon B, comfilcon A and samfilcon A) were incubated in an artificial tear solution (ATS) for up to 30 days. CL incubation was alternated between the ATS (16 hours) and a multipurpose care regimen (8 hours). The ATS included fluorescently tagged cholesteryl ester (5-cholesten-3ß-ol 6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]caproate; CE-NBD) and confocal laser scanning microscopy visualized the distribution of the lipid through the CLs.Results: The distribution of CE-NBD was homogenous from the anterior to posterior surface in senofilcon C and comfilcon A, at all time points. For lotrafilcon B and samfilcon A, CE-NBD localization was heterogeneous, with greater amounts on the surfaces on Day 1 and Day 14 compared to the lens matrix; however, differences in concentration between the surface and bulk diminished by Day 30.Conclusion: The distribution of the non-polar lipid CE-NBD varied with lens material chemistry. While some lens materials deposited the lipid primarily on the surface after 16 hours of exposure, all materials exhibited a homogenous distribution after one month.Keywords: lipid distribution, silicone hydrogel contact lenses, cholesteryl ester, artificial tear solution
format	article
author	Qiao H Luensmann D Heynen M Drolle E Subbaraman LN Scales C Riederer D Fadli Z Jones L
author_facet	Qiao H Luensmann D Heynen M Drolle E Subbaraman LN Scales C Riederer D Fadli Z Jones L
author_sort	Qiao H
title	In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials
title_short	In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials
title_full	In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials
title_fullStr	In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials
title_full_unstemmed	In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials
title_sort	in vitro evaluation of the location of cholesteryl ester deposits on monthly replacement silicone hydrogel contact lens materials
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/bf5eef6a6f1645e3906e1ceeb5a9d255
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In vitro Evaluation of the Location of Cholesteryl Ester Deposits on Monthly Replacement Silicone Hydrogel Contact Lens Materials

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