Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification

3-Methoxyphencyclidine (3-MeO-PCP) is a new psychoactive substance that belongs to the phencyclidines family, first identified in Europe in 2012. This drug presents a stronger binding to N-methyl-D-aspartate (NMDA) receptors when compared to phencyclidine, which results in more potent effects, even...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nadia Arbouche, Pascal Kintz, Cecile Zagdoun, Laurie Gheddar, Jean-Sébastien Raul, Alice Ameline
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	forensic sciences forensic toxicology 3-meo-pcp metabolites fatal intoxication blood urine Criminal law and procedure K5000-5582 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/bf67857d6b9f4aeb9ee98f4566e6b1f6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bf67857d6b9f4aeb9ee98f4566e6b1f6
record_format	dspace
spelling	oai:doaj.org-article:bf67857d6b9f4aeb9ee98f4566e6b1f62021-12-01T14:40:59ZDetermination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification2096-17902471-141110.1080/20961790.2021.1928821https://doaj.org/article/bf67857d6b9f4aeb9ee98f4566e6b1f62021-07-01T00:00:00Zhttp://dx.doi.org/10.1080/20961790.2021.1928821https://doaj.org/toc/2096-1790https://doaj.org/toc/2471-14113-Methoxyphencyclidine (3-MeO-PCP) is a new psychoactive substance that belongs to the phencyclidines family, first identified in Europe in 2012. This drug presents a stronger binding to N-methyl-D-aspartate (NMDA) receptors when compared to phencyclidine, which results in more potent effects, even at low concentrations. Very few articles have been published regarding 3-MeO-PCP in forensic toxicology. In this paper, the authors present a fatal 3-MeO-PCP intoxication case. In addition to the detection of the parent drug, metabolites were investigated in urine and, for the first time in the scientific literature, in blood. 3-MeO-PCP and its metabolites were quantitated by liquid chromatography-tandem mass spectrometry system (LC-MS/MS). Identification was confirmed by liquid chromatography-high resolution mass spectrometry (LC-HRMS). 3-MeO-PCP tested positive in femoral blood (3 525 ng/mL) and urine (7 384 ng/mL). The femoral blood concentration was higher than the fatal concentrations range already reported in the literature (from 50 to 3 200 ng/mL). 3-MeO-PCP metabolites, including O-demethyl-3-MeO-PCP, piperidine-OH-3-MeO-PCP, O-demethyl-piperidine-di-OH-3-MeO-PCP and piperidine-di-OH-3-MeO-PCP, were detected in blood. In addition, two new metabolites, O-demethyl-piperidine-OH-3-MeO-PCP and O-demethyl-cyclohexyl-OH, were identified in both blood and urine. Unfortunately, due to the lack of reference material on the market, it was not possible to measure the concentration of these metabolites. However, the ratios between the metabolites and the parent drug were useful to estimate their analytical response and prevalence. At this time, considering the low ratios (<1) between metabolites and parent drug, metabolites testing does not seem useful to increase the detection window of the drug.Nadia ArbouchePascal KintzCecile ZagdounLaurie GheddarJean-Sébastien RaulAlice AmelineTaylor & Francis Grouparticleforensic sciencesforensic toxicology3-meo-pcpmetabolitesfatal intoxicationbloodurineCriminal law and procedureK5000-5582Public aspects of medicineRA1-1270ENForensic Sciences Research, Vol 6, Iss 3, Pp 208-214 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	forensic sciences forensic toxicology 3-meo-pcp metabolites fatal intoxication blood urine Criminal law and procedure K5000-5582 Public aspects of medicine RA1-1270
spellingShingle	forensic sciences forensic toxicology 3-meo-pcp metabolites fatal intoxication blood urine Criminal law and procedure K5000-5582 Public aspects of medicine RA1-1270 Nadia Arbouche Pascal Kintz Cecile Zagdoun Laurie Gheddar Jean-Sébastien Raul Alice Ameline Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification
description	3-Methoxyphencyclidine (3-MeO-PCP) is a new psychoactive substance that belongs to the phencyclidines family, first identified in Europe in 2012. This drug presents a stronger binding to N-methyl-D-aspartate (NMDA) receptors when compared to phencyclidine, which results in more potent effects, even at low concentrations. Very few articles have been published regarding 3-MeO-PCP in forensic toxicology. In this paper, the authors present a fatal 3-MeO-PCP intoxication case. In addition to the detection of the parent drug, metabolites were investigated in urine and, for the first time in the scientific literature, in blood. 3-MeO-PCP and its metabolites were quantitated by liquid chromatography-tandem mass spectrometry system (LC-MS/MS). Identification was confirmed by liquid chromatography-high resolution mass spectrometry (LC-HRMS). 3-MeO-PCP tested positive in femoral blood (3 525 ng/mL) and urine (7 384 ng/mL). The femoral blood concentration was higher than the fatal concentrations range already reported in the literature (from 50 to 3 200 ng/mL). 3-MeO-PCP metabolites, including O-demethyl-3-MeO-PCP, piperidine-OH-3-MeO-PCP, O-demethyl-piperidine-di-OH-3-MeO-PCP and piperidine-di-OH-3-MeO-PCP, were detected in blood. In addition, two new metabolites, O-demethyl-piperidine-OH-3-MeO-PCP and O-demethyl-cyclohexyl-OH, were identified in both blood and urine. Unfortunately, due to the lack of reference material on the market, it was not possible to measure the concentration of these metabolites. However, the ratios between the metabolites and the parent drug were useful to estimate their analytical response and prevalence. At this time, considering the low ratios (<1) between metabolites and parent drug, metabolites testing does not seem useful to increase the detection window of the drug.
format	article
author	Nadia Arbouche Pascal Kintz Cecile Zagdoun Laurie Gheddar Jean-Sébastien Raul Alice Ameline
author_facet	Nadia Arbouche Pascal Kintz Cecile Zagdoun Laurie Gheddar Jean-Sébastien Raul Alice Ameline
author_sort	Nadia Arbouche
title	Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification
title_short	Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification
title_full	Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification
title_fullStr	Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification
title_full_unstemmed	Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification
title_sort	determination of 3-meo-pcp in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/bf67857d6b9f4aeb9ee98f4566e6b1f6
work_keys_str_mv	AT nadiaarbouche determinationof3meopcpinhumanbloodandurineinafatalintoxicationcasewithaspecificfocusonmetabolitesidentification AT pascalkintz determinationof3meopcpinhumanbloodandurineinafatalintoxicationcasewithaspecificfocusonmetabolitesidentification AT cecilezagdoun determinationof3meopcpinhumanbloodandurineinafatalintoxicationcasewithaspecificfocusonmetabolitesidentification AT lauriegheddar determinationof3meopcpinhumanbloodandurineinafatalintoxicationcasewithaspecificfocusonmetabolitesidentification AT jeansebastienraul determinationof3meopcpinhumanbloodandurineinafatalintoxicationcasewithaspecificfocusonmetabolitesidentification AT aliceameline determinationof3meopcpinhumanbloodandurineinafatalintoxicationcasewithaspecificfocusonmetabolitesidentification
_version_	1718405017611796480

Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case, with a specific focus on metabolites identification

Ejemplares similares