Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.

Axonal outgrowth and the formation of the axon initial segment (AIS) are early events in the acquisition of neuronal polarity. The AIS is characterized by a high concentration of voltage-dependent sodium and potassium channels. However, the specific ion channel subunits present and their precise loc...

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Autores principales:	Diana Sánchez-Ponce, Javier DeFelipe, Juan José Garrido, Alberto Muñoz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf68b7fa36a04538b08ae47059a9d453
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spelling	oai:doaj.org-article:bf68b7fa36a04538b08ae47059a9d4532021-11-18T08:10:20ZDevelopmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.1932-620310.1371/journal.pone.0048557https://doaj.org/article/bf68b7fa36a04538b08ae47059a9d4532012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23119056/?tool=EBIhttps://doaj.org/toc/1932-6203Axonal outgrowth and the formation of the axon initial segment (AIS) are early events in the acquisition of neuronal polarity. The AIS is characterized by a high concentration of voltage-dependent sodium and potassium channels. However, the specific ion channel subunits present and their precise localization in this axonal subdomain vary both during development and among the types of neurons, probably determining their firing characteristics in response to stimulation. Here, we characterize the developmental expression of different subfamilies of voltage-gated potassium channels in the AISs of cultured mouse hippocampal neurons, including subunits Kv1.2, Kv2.2 and Kv7.2. In contrast to the early appearance of voltage-gated sodium channels and the Kv7.2 subunit at the AIS, Kv1.2 and Kv2.2 subunits were tethered at the AIS only after 10 days in vitro. Interestingly, we observed different patterns of Kv1.2 and Kv2.2 subunit expression, with each confined to distinct neuronal populations. The accumulation of Kv1.2 and Kv2.2 subunits at the AIS was dependent on ankyrin G tethering, it was not affected by disruption of the actin cytoskeleton and it was resistant to detergent extraction, as described previously for other AIS proteins. This distribution of potassium channels in the AIS further emphasizes the heterogeneity of this structure in different neuronal populations, as proposed previously, and suggests corresponding differences in action potential regulation.Diana Sánchez-PonceJavier DeFelipeJuan José GarridoAlberto MuñozPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48557 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diana Sánchez-Ponce Javier DeFelipe Juan José Garrido Alberto Muñoz Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.
description	Axonal outgrowth and the formation of the axon initial segment (AIS) are early events in the acquisition of neuronal polarity. The AIS is characterized by a high concentration of voltage-dependent sodium and potassium channels. However, the specific ion channel subunits present and their precise localization in this axonal subdomain vary both during development and among the types of neurons, probably determining their firing characteristics in response to stimulation. Here, we characterize the developmental expression of different subfamilies of voltage-gated potassium channels in the AISs of cultured mouse hippocampal neurons, including subunits Kv1.2, Kv2.2 and Kv7.2. In contrast to the early appearance of voltage-gated sodium channels and the Kv7.2 subunit at the AIS, Kv1.2 and Kv2.2 subunits were tethered at the AIS only after 10 days in vitro. Interestingly, we observed different patterns of Kv1.2 and Kv2.2 subunit expression, with each confined to distinct neuronal populations. The accumulation of Kv1.2 and Kv2.2 subunits at the AIS was dependent on ankyrin G tethering, it was not affected by disruption of the actin cytoskeleton and it was resistant to detergent extraction, as described previously for other AIS proteins. This distribution of potassium channels in the AIS further emphasizes the heterogeneity of this structure in different neuronal populations, as proposed previously, and suggests corresponding differences in action potential regulation.
format	article
author	Diana Sánchez-Ponce Javier DeFelipe Juan José Garrido Alberto Muñoz
author_facet	Diana Sánchez-Ponce Javier DeFelipe Juan José Garrido Alberto Muñoz
author_sort	Diana Sánchez-Ponce
title	Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.
title_short	Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.
title_full	Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.
title_fullStr	Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.
title_full_unstemmed	Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.
title_sort	developmental expression of kv potassium channels at the axon initial segment of cultured hippocampal neurons.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bf68b7fa36a04538b08ae47059a9d453
work_keys_str_mv	AT dianasanchezponce developmentalexpressionofkvpotassiumchannelsattheaxoninitialsegmentofculturedhippocampalneurons AT javierdefelipe developmentalexpressionofkvpotassiumchannelsattheaxoninitialsegmentofculturedhippocampalneurons AT juanjosegarrido developmentalexpressionofkvpotassiumchannelsattheaxoninitialsegmentofculturedhippocampalneurons AT albertomunoz developmentalexpressionofkvpotassiumchannelsattheaxoninitialsegmentofculturedhippocampalneurons
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Developmental expression of Kv potassium channels at the axon initial segment of cultured hippocampal neurons.

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