Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis

Danlou tablet (DLT), a commercial Chinese patent medicine, has been widely used to treat cardiovascular diseases for many years. Atherosclerosis (AS) is the leading cause of cardiovascular disease. Increasing evidence indicates that autophagy plays a vital role in the development of AS. Here we inve...

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Autores principales:	Li Wang, Tong Wu, Chunying Si, He Wang, Ke Yue, Shasha Shang, Xiaohui Li, Yushan Chen, Huaimin Guan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	atherosclerosis danlou tablet autophagy PI3K/AKT/mTOR Chinese patent medicine Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/bf6afad25e8449d7a812bff9b5448a7f
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spelling	oai:doaj.org-article:bf6afad25e8449d7a812bff9b5448a7f2021-11-18T09:40:11ZDanlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis1663-981210.3389/fphar.2021.730525https://doaj.org/article/bf6afad25e8449d7a812bff9b5448a7f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.730525/fullhttps://doaj.org/toc/1663-9812Danlou tablet (DLT), a commercial Chinese patent medicine, has been widely used to treat cardiovascular diseases for many years. Atherosclerosis (AS) is the leading cause of cardiovascular disease. Increasing evidence indicates that autophagy plays a vital role in the development of AS. Here we investigated whether DLT could activate autophagy to improve AS and further clarified its underlying mechanisms. In an ApoE−/− mice model, the results of Oil red O, Masson’s trichrome, and H&E staining techniques showed that DLT significantly inhibited lipid accumulation and fibrosis formation in atherosclerotic plaque tissue. DLT also inhibited serum triglyceride, cholesterol, and low-density lipoprotein levels and suppressed serum levels of inflammatory factors interleukin-6 and tumor necrosis factor-α in ApoE−/− mice. Moreover, DLT suppressed proliferation, migration, and invasion of human vascular adventitial fibroblasts (HVAFs) by inhibiting the PI3K/Akt/mTOR pathway. In addition, western blot analysis showed that Danlou tablet treatment decreased the expression of p62 and increased Beclin 1 and LC3 I -to-LC3 II ratios in HVAFs. The role of autophagy in treating atherosclerosis by DLT is confirmed by 3-methyladenine (autophagy inhibitor) and rapamycin (autophagy activator) in HVAFs. In summary, DLT activated PI3K/Akt/mTOR-mediated autophagy of vascular adventitial fibroblasts to protect cells from damage caused by atherosclerosis.Li WangTong WuChunying SiHe WangKe YueShasha ShangXiaohui LiYushan ChenHuaimin GuanFrontiers Media S.A.articleatherosclerosisdanlou tabletautophagyPI3K/AKT/mTORChinese patent medicineTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	atherosclerosis danlou tablet autophagy PI3K/AKT/mTOR Chinese patent medicine Therapeutics. Pharmacology RM1-950
spellingShingle	atherosclerosis danlou tablet autophagy PI3K/AKT/mTOR Chinese patent medicine Therapeutics. Pharmacology RM1-950 Li Wang Tong Wu Chunying Si He Wang Ke Yue Shasha Shang Xiaohui Li Yushan Chen Huaimin Guan Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
description	Danlou tablet (DLT), a commercial Chinese patent medicine, has been widely used to treat cardiovascular diseases for many years. Atherosclerosis (AS) is the leading cause of cardiovascular disease. Increasing evidence indicates that autophagy plays a vital role in the development of AS. Here we investigated whether DLT could activate autophagy to improve AS and further clarified its underlying mechanisms. In an ApoE−/− mice model, the results of Oil red O, Masson’s trichrome, and H&E staining techniques showed that DLT significantly inhibited lipid accumulation and fibrosis formation in atherosclerotic plaque tissue. DLT also inhibited serum triglyceride, cholesterol, and low-density lipoprotein levels and suppressed serum levels of inflammatory factors interleukin-6 and tumor necrosis factor-α in ApoE−/− mice. Moreover, DLT suppressed proliferation, migration, and invasion of human vascular adventitial fibroblasts (HVAFs) by inhibiting the PI3K/Akt/mTOR pathway. In addition, western blot analysis showed that Danlou tablet treatment decreased the expression of p62 and increased Beclin 1 and LC3 I -to-LC3 II ratios in HVAFs. The role of autophagy in treating atherosclerosis by DLT is confirmed by 3-methyladenine (autophagy inhibitor) and rapamycin (autophagy activator) in HVAFs. In summary, DLT activated PI3K/Akt/mTOR-mediated autophagy of vascular adventitial fibroblasts to protect cells from damage caused by atherosclerosis.
format	article
author	Li Wang Tong Wu Chunying Si He Wang Ke Yue Shasha Shang Xiaohui Li Yushan Chen Huaimin Guan
author_facet	Li Wang Tong Wu Chunying Si He Wang Ke Yue Shasha Shang Xiaohui Li Yushan Chen Huaimin Guan
author_sort	Li Wang
title	Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
title_short	Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
title_full	Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
title_fullStr	Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
title_full_unstemmed	Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
title_sort	danlou tablet activates autophagy of vascular adventitial fibroblasts through pi3k/akt/mtor to protect cells from damage caused by atherosclerosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/bf6afad25e8449d7a812bff9b5448a7f
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Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis

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