Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.

In this study, we applied multiple reaction monitoring (MRM)-profiling to explore the relative ion intensity of lipid classes in plasma samples from sea turtles in order to profile lipids relevant to sea turtle physiology and investigate how dynamic ocean environments affect these profiles. We colle...

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Autores principales:	Chelsea E Clyde-Brockway, Christina R Ferreira, Elizabeth A Flaherty, Frank V Paladino
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf6c89081806403fbb09853bc49cb319
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spelling	oai:doaj.org-article:bf6c89081806403fbb09853bc49cb3192021-12-02T20:06:52ZLipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.1932-620310.1371/journal.pone.0253916https://doaj.org/article/bf6c89081806403fbb09853bc49cb3192021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253916https://doaj.org/toc/1932-6203In this study, we applied multiple reaction monitoring (MRM)-profiling to explore the relative ion intensity of lipid classes in plasma samples from sea turtles in order to profile lipids relevant to sea turtle physiology and investigate how dynamic ocean environments affect these profiles. We collected plasma samples from foraging green (Chelonia mydas, n = 28) and hawksbill (Eretmochelys imbricata, n = 16) turtles live captured in North Pacific Costa Rica in 2017. From these samples, we identified 623 MRMs belonging to 10 lipid classes (sphingomyelin, phosphatidylcholine, free fatty acid, cholesteryl ester, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, phosphatidylethanolamine, ceramide, and triacylglyceride) and one metabolite group (acyl-carnitine) present in sea turtle plasma. The relative ion intensities of most lipids (80%) were consistent between species, across seasons, and were not correlated to body size or estimated sex. Of the differences we observed, the most pronounced was the differences in relative ion intensity between species. We identified 123 lipids that had species-specific relative ion intensities. While some of this variability is likely due to green and hawksbill turtles consuming different food items, we found indications of a phylogenetic component as well. Of these, we identified 47 lipids that varied by season, most belonging to the structural phospholipid classes. Overall, more lipids (n = 39) had higher relative ion intensity in the upwelling (colder) season compared to the non-upwelling season (n = 8). Further, we found more variability in hawksbill turtles than green turtles. Here, we provide the framework in which to apply future lipid profiling in the assessment of health, physiology, and behavior in endangered sea turtles.Chelsea E Clyde-BrockwayChristina R FerreiraElizabeth A FlahertyFrank V PaladinoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0253916 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chelsea E Clyde-Brockway Christina R Ferreira Elizabeth A Flaherty Frank V Paladino Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.
description	In this study, we applied multiple reaction monitoring (MRM)-profiling to explore the relative ion intensity of lipid classes in plasma samples from sea turtles in order to profile lipids relevant to sea turtle physiology and investigate how dynamic ocean environments affect these profiles. We collected plasma samples from foraging green (Chelonia mydas, n = 28) and hawksbill (Eretmochelys imbricata, n = 16) turtles live captured in North Pacific Costa Rica in 2017. From these samples, we identified 623 MRMs belonging to 10 lipid classes (sphingomyelin, phosphatidylcholine, free fatty acid, cholesteryl ester, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, phosphatidylethanolamine, ceramide, and triacylglyceride) and one metabolite group (acyl-carnitine) present in sea turtle plasma. The relative ion intensities of most lipids (80%) were consistent between species, across seasons, and were not correlated to body size or estimated sex. Of the differences we observed, the most pronounced was the differences in relative ion intensity between species. We identified 123 lipids that had species-specific relative ion intensities. While some of this variability is likely due to green and hawksbill turtles consuming different food items, we found indications of a phylogenetic component as well. Of these, we identified 47 lipids that varied by season, most belonging to the structural phospholipid classes. Overall, more lipids (n = 39) had higher relative ion intensity in the upwelling (colder) season compared to the non-upwelling season (n = 8). Further, we found more variability in hawksbill turtles than green turtles. Here, we provide the framework in which to apply future lipid profiling in the assessment of health, physiology, and behavior in endangered sea turtles.
format	article
author	Chelsea E Clyde-Brockway Christina R Ferreira Elizabeth A Flaherty Frank V Paladino
author_facet	Chelsea E Clyde-Brockway Christina R Ferreira Elizabeth A Flaherty Frank V Paladino
author_sort	Chelsea E Clyde-Brockway
title	Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.
title_short	Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.
title_full	Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.
title_fullStr	Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.
title_full_unstemmed	Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.
title_sort	lipid profiling suggests species specificity and minimal seasonal variation in pacific green and hawksbill turtle plasma.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/bf6c89081806403fbb09853bc49cb319
work_keys_str_mv	AT chelseaeclydebrockway lipidprofilingsuggestsspeciesspecificityandminimalseasonalvariationinpacificgreenandhawksbillturtleplasma AT christinarferreira lipidprofilingsuggestsspeciesspecificityandminimalseasonalvariationinpacificgreenandhawksbillturtleplasma AT elizabethaflaherty lipidprofilingsuggestsspeciesspecificityandminimalseasonalvariationinpacificgreenandhawksbillturtleplasma AT frankvpaladino lipidprofilingsuggestsspeciesspecificityandminimalseasonalvariationinpacificgreenandhawksbillturtleplasma
_version_	1718375326170480640

Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.

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