From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients

<h4>Objective</h4> To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in...

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Autores principales: Lesla E. S. Bruijnesteijn van Coppenraet, Jacky Flipse, Janny A. Wallinga, Marloes Vermeer, Wil A. van der Reijden, Jan F. L. Weel, Adri G. M. van der Zanden, Theo A. Schuurs, Gijs J. H. M. Ruijs
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Publicado: Public Library of Science (PLoS) 2021
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spelling oai:doaj.org-article:bf748af38fc9462d8f5faf4a729941832021-11-11T07:14:46ZFrom a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients1932-6203https://doaj.org/article/bf748af38fc9462d8f5faf4a729941832021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565752/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4> To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in the Netherlands (2010–2012). <h4>Methods</h4> Archived stool samples from 1340 cases and 1100 controls were retested using internally controlled multiplex real-time PCRs for putative pathogenic gastroenteritis viruses: adenovirus, astrovirus, bocavirus, enterovirus, norovirus GI and GII, human parechovirus, rotavirus, salivirus, sapovirus, and torovirus. <h4>Results</h4> The prevalence of any virus in symptomatic cases and asymptomatic controls was 16.6% (223/1340) and 10.2% (112/1100), respectively. Prevalence of astrovirus (adjusted odds ratio (aOR) 10.37; 95% confidence interval (CI) 1.34–80.06) and norovirus GII (aOR 3.10; CI 1.62–5.92) was significantly higher in cases versus controls. Rotavirus was encountered only in cases. We did not find torovirus and there was no statistically significant relationship with cases for salivirus (aOR 1,67; (CI) 0.43–6.54)), adenovirus non-group F (aOR 1.20; CI 0.75–1.91), bocavirus (aOR 0.85; CI 0.05–13.64), enterovirus (aOR 0.83; CI 0.50–1.37), human parechovirus (aOR 1.61; CI 0.54–4.77) and sapovirus (aOR 1.15; CI 0.67–1.98). Though adenovirus group F (aOR 6.37; CI 0.80–50.92) and norovirus GI (aOR 2.22, CI: 0.79–6.23) are known enteropathogenic viruses and were more prevalent in cases than in controls, this did not reach significance in this study. The Ct value did not discriminate between carriage and disease in PCR-positive subjects. <h4>Conclusions</h4> In our population, diagnostic gastroenteritis tests should screen for adenovirus group F, astrovirus, noroviruses GI and GII, and rotavirus. Case-control studies as ours are lacking and should also be carried out in populations from other epidemiological backgrounds.Lesla E. S. Bruijnesteijn van CoppenraetJacky FlipseJanny A. WallingaMarloes VermeerWil A. van der ReijdenJan F. L. WeelAdri G. M. van der ZandenTheo A. SchuursGijs J. H. M. RuijsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lesla E. S. Bruijnesteijn van Coppenraet
Jacky Flipse
Janny A. Wallinga
Marloes Vermeer
Wil A. van der Reijden
Jan F. L. Weel
Adri G. M. van der Zanden
Theo A. Schuurs
Gijs J. H. M. Ruijs
From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients
description <h4>Objective</h4> To evaluate the pathogenicity of a broad range of 11 possible gastroenteritis viruses, by means of statistical relationships with cases vs. controls, or Ct-values, in order to establish the most appropriate diagnostic panel for our general practitioner (GP) patients in the Netherlands (2010–2012). <h4>Methods</h4> Archived stool samples from 1340 cases and 1100 controls were retested using internally controlled multiplex real-time PCRs for putative pathogenic gastroenteritis viruses: adenovirus, astrovirus, bocavirus, enterovirus, norovirus GI and GII, human parechovirus, rotavirus, salivirus, sapovirus, and torovirus. <h4>Results</h4> The prevalence of any virus in symptomatic cases and asymptomatic controls was 16.6% (223/1340) and 10.2% (112/1100), respectively. Prevalence of astrovirus (adjusted odds ratio (aOR) 10.37; 95% confidence interval (CI) 1.34–80.06) and norovirus GII (aOR 3.10; CI 1.62–5.92) was significantly higher in cases versus controls. Rotavirus was encountered only in cases. We did not find torovirus and there was no statistically significant relationship with cases for salivirus (aOR 1,67; (CI) 0.43–6.54)), adenovirus non-group F (aOR 1.20; CI 0.75–1.91), bocavirus (aOR 0.85; CI 0.05–13.64), enterovirus (aOR 0.83; CI 0.50–1.37), human parechovirus (aOR 1.61; CI 0.54–4.77) and sapovirus (aOR 1.15; CI 0.67–1.98). Though adenovirus group F (aOR 6.37; CI 0.80–50.92) and norovirus GI (aOR 2.22, CI: 0.79–6.23) are known enteropathogenic viruses and were more prevalent in cases than in controls, this did not reach significance in this study. The Ct value did not discriminate between carriage and disease in PCR-positive subjects. <h4>Conclusions</h4> In our population, diagnostic gastroenteritis tests should screen for adenovirus group F, astrovirus, noroviruses GI and GII, and rotavirus. Case-control studies as ours are lacking and should also be carried out in populations from other epidemiological backgrounds.
format article
author Lesla E. S. Bruijnesteijn van Coppenraet
Jacky Flipse
Janny A. Wallinga
Marloes Vermeer
Wil A. van der Reijden
Jan F. L. Weel
Adri G. M. van der Zanden
Theo A. Schuurs
Gijs J. H. M. Ruijs
author_facet Lesla E. S. Bruijnesteijn van Coppenraet
Jacky Flipse
Janny A. Wallinga
Marloes Vermeer
Wil A. van der Reijden
Jan F. L. Weel
Adri G. M. van der Zanden
Theo A. Schuurs
Gijs J. H. M. Ruijs
author_sort Lesla E. S. Bruijnesteijn van Coppenraet
title From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients
title_short From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients
title_full From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients
title_fullStr From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients
title_full_unstemmed From a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients
title_sort from a case-control survey to a diagnostic viral gastroenteritis panel for testing of general practitioners’ patients
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/bf748af38fc9462d8f5faf4a72994183
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