The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.

Repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) requires that the 5'-terminated DNA strands are resected to generate single-stranded DNA overhangs. This process is initiated by a short-range resection catalyzed by the MRX (Mre11-Rad50-Xrs2) complex, which is followed...

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Autores principales:	Marco Gnugnoli, Erika Casari, Maria Pia Longhese
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/bf785901828e4d50aea3d20a881a9212
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spelling	oai:doaj.org-article:bf785901828e4d50aea3d20a881a92122021-12-02T20:03:20ZThe chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.1553-73901553-740410.1371/journal.pgen.1009807https://doaj.org/article/bf785901828e4d50aea3d20a881a92122021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009807https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) requires that the 5'-terminated DNA strands are resected to generate single-stranded DNA overhangs. This process is initiated by a short-range resection catalyzed by the MRX (Mre11-Rad50-Xrs2) complex, which is followed by a long-range step involving the nucleases Exo1 and Dna2. Here we show that the Saccharomyces cerevisiae ATP-dependent chromatin-remodeling protein Chd1 participates in both short- and long-range resection by promoting MRX and Exo1 association with the DSB ends. Furthermore, Chd1 reduces histone occupancy near the DSB ends and promotes DSB repair by HR. All these functions require Chd1 ATPase activity, supporting a role for Chd1 in the opening of chromatin at the DSB site to facilitate MRX and Exo1 processing activities.Marco GnugnoliErika CasariMaria Pia LonghesePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009807 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Marco Gnugnoli Erika Casari Maria Pia Longhese The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.
description	Repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) requires that the 5'-terminated DNA strands are resected to generate single-stranded DNA overhangs. This process is initiated by a short-range resection catalyzed by the MRX (Mre11-Rad50-Xrs2) complex, which is followed by a long-range step involving the nucleases Exo1 and Dna2. Here we show that the Saccharomyces cerevisiae ATP-dependent chromatin-remodeling protein Chd1 participates in both short- and long-range resection by promoting MRX and Exo1 association with the DSB ends. Furthermore, Chd1 reduces histone occupancy near the DSB ends and promotes DSB repair by HR. All these functions require Chd1 ATPase activity, supporting a role for Chd1 in the opening of chromatin at the DSB site to facilitate MRX and Exo1 processing activities.
format	article
author	Marco Gnugnoli Erika Casari Maria Pia Longhese
author_facet	Marco Gnugnoli Erika Casari Maria Pia Longhese
author_sort	Marco Gnugnoli
title	The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.
title_short	The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.
title_full	The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.
title_fullStr	The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.
title_full_unstemmed	The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.
title_sort	chromatin remodeler chd1 supports mrx and exo1 functions in resection of dna double-strand breaks.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/bf785901828e4d50aea3d20a881a9212
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The chromatin remodeler Chd1 supports MRX and Exo1 functions in resection of DNA double-strand breaks.

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