Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?

Lactate dehydrogenase (LDH) has been shown to play an important role in adaptation of several aquatic species to different habitats. The genomes of Daphnia pulex, a pond species, and Daphnia pulicaria, a lake inhabitant, encode two L-LDH enzymes, LDHA and LDHB. We estimated relative levels of Ldh ge...

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Autores principales:	Melania E Cristescu, Bora Demiri, Ianina Altshuler, Teresa J Crease
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf787567a45c4b25af1d16fa933cf1bb
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spelling	oai:doaj.org-article:bf787567a45c4b25af1d16fa933cf1bb2021-11-25T06:06:29ZGene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?1932-620310.1371/journal.pone.0103964https://doaj.org/article/bf787567a45c4b25af1d16fa933cf1bb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25080082/?tool=EBIhttps://doaj.org/toc/1932-6203Lactate dehydrogenase (LDH) has been shown to play an important role in adaptation of several aquatic species to different habitats. The genomes of Daphnia pulex, a pond species, and Daphnia pulicaria, a lake inhabitant, encode two L-LDH enzymes, LDHA and LDHB. We estimated relative levels of Ldh gene expression in these two closely related species and their hybrids in four environmental settings, each characterized by one of two temperatures (10°C or 20°C), and one of two concentrations of dissolved oxygen (DO; 6.5-7 mg/l or 2-3 mg/l). We found that levels of LdhA expression were 4 to 48 times higher than LdhB expression (p<0.005) in all three groups (the two parental species and hybrids). Moreover, levels of LdhB expression differed significantly (p<0.05) between D. pulex and D. pulicaria, but neither species differed from the hybrid. Consistently higher expression of LdhA relative to LdhB in both species and the hybrid suggests that the two isozymes could be performing different functions. No significant differences in levels of gene expression were observed among the four combinations of temperature and dissolved oxygen (p>0.1). Given that Daphnia dwell in environments characterized by fluctuating conditions with long periods of low dissolved oxygen concentration, we suggest that these species could employ regulated metabolic depression to survive in such environments.Melania E CristescuBora DemiriIanina AltshulerTeresa J CreasePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e103964 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Melania E Cristescu Bora Demiri Ianina Altshuler Teresa J Crease Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?
description	Lactate dehydrogenase (LDH) has been shown to play an important role in adaptation of several aquatic species to different habitats. The genomes of Daphnia pulex, a pond species, and Daphnia pulicaria, a lake inhabitant, encode two L-LDH enzymes, LDHA and LDHB. We estimated relative levels of Ldh gene expression in these two closely related species and their hybrids in four environmental settings, each characterized by one of two temperatures (10°C or 20°C), and one of two concentrations of dissolved oxygen (DO; 6.5-7 mg/l or 2-3 mg/l). We found that levels of LdhA expression were 4 to 48 times higher than LdhB expression (p<0.005) in all three groups (the two parental species and hybrids). Moreover, levels of LdhB expression differed significantly (p<0.05) between D. pulex and D. pulicaria, but neither species differed from the hybrid. Consistently higher expression of LdhA relative to LdhB in both species and the hybrid suggests that the two isozymes could be performing different functions. No significant differences in levels of gene expression were observed among the four combinations of temperature and dissolved oxygen (p>0.1). Given that Daphnia dwell in environments characterized by fluctuating conditions with long periods of low dissolved oxygen concentration, we suggest that these species could employ regulated metabolic depression to survive in such environments.
format	article
author	Melania E Cristescu Bora Demiri Ianina Altshuler Teresa J Crease
author_facet	Melania E Cristescu Bora Demiri Ianina Altshuler Teresa J Crease
author_sort	Melania E Cristescu
title	Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?
title_short	Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?
title_full	Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?
title_fullStr	Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?
title_full_unstemmed	Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?
title_sort	gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bf787567a45c4b25af1d16fa933cf1bb
work_keys_str_mv	AT melaniaecristescu geneexpressionvariationinduplicatelactatedehydrogenasegenesdoecologicalspeciesshowdistinctresponses AT borademiri geneexpressionvariationinduplicatelactatedehydrogenasegenesdoecologicalspeciesshowdistinctresponses AT ianinaaltshuler geneexpressionvariationinduplicatelactatedehydrogenasegenesdoecologicalspeciesshowdistinctresponses AT teresajcrease geneexpressionvariationinduplicatelactatedehydrogenasegenesdoecologicalspeciesshowdistinctresponses
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Gene expression variation in duplicate lactate dehydrogenase genes: do ecological species show distinct responses?

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