Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy

Abstract Muscle atrophy occurs in a variety of physiological and pathological conditions. Specific molecular networks that govern protein synthesis and degradation play important roles in controlling muscle mass under diverse catabolic states. MicroRNAs (miRNAs) were previously found to be regulator...

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Autores principales:	Satoshi Oikawa, Jaehoon Shin, Takao Akama, Takayuki Akimoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf7bca035e6e4290ba7afcaaf3ebffc5
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spelling	oai:doaj.org-article:bf7bca035e6e4290ba7afcaaf3ebffc52021-12-02T19:16:59ZDicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy10.1038/s41598-021-98545-02045-2322https://doaj.org/article/bf7bca035e6e4290ba7afcaaf3ebffc52021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98545-0https://doaj.org/toc/2045-2322Abstract Muscle atrophy occurs in a variety of physiological and pathological conditions. Specific molecular networks that govern protein synthesis and degradation play important roles in controlling muscle mass under diverse catabolic states. MicroRNAs (miRNAs) were previously found to be regulators of protein synthesis and degradation, and their expressions in skeletal muscle were altered in muscle wasting conditions. However, functional roles of miRNAs in muscle atrophy are poorly understood. In this study, we generated tamoxifen-inducible Dicer knockout (iDicer KO) mice and subjected them to 2 weeks of single hindlimb denervation. The expression of Dicer mRNA was significantly reduced in muscle of the iDicer KO mice compared to that of WT mice. The loss of Dicer moderately reduced levels of muscle-enriched miRNAs, miR-1, miR-133a and miR-206 in both innervated and denervated muscles of the iDicer KO mice. We also found that the extent of denervation-induced muscle atrophy as well as changes of signaling molecules related to protein synthesis/degradation pathways in the iDicer KO mice were comparable to these in WT mice. Taken together, Dicer knockout in adult skeletal muscle did not affect denervation-induced muscle atrophy.Satoshi OikawaJaehoon ShinTakao AkamaTakayuki AkimotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoshi Oikawa Jaehoon Shin Takao Akama Takayuki Akimoto Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy
description	Abstract Muscle atrophy occurs in a variety of physiological and pathological conditions. Specific molecular networks that govern protein synthesis and degradation play important roles in controlling muscle mass under diverse catabolic states. MicroRNAs (miRNAs) were previously found to be regulators of protein synthesis and degradation, and their expressions in skeletal muscle were altered in muscle wasting conditions. However, functional roles of miRNAs in muscle atrophy are poorly understood. In this study, we generated tamoxifen-inducible Dicer knockout (iDicer KO) mice and subjected them to 2 weeks of single hindlimb denervation. The expression of Dicer mRNA was significantly reduced in muscle of the iDicer KO mice compared to that of WT mice. The loss of Dicer moderately reduced levels of muscle-enriched miRNAs, miR-1, miR-133a and miR-206 in both innervated and denervated muscles of the iDicer KO mice. We also found that the extent of denervation-induced muscle atrophy as well as changes of signaling molecules related to protein synthesis/degradation pathways in the iDicer KO mice were comparable to these in WT mice. Taken together, Dicer knockout in adult skeletal muscle did not affect denervation-induced muscle atrophy.
format	article
author	Satoshi Oikawa Jaehoon Shin Takao Akama Takayuki Akimoto
author_facet	Satoshi Oikawa Jaehoon Shin Takao Akama Takayuki Akimoto
author_sort	Satoshi Oikawa
title	Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy
title_short	Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy
title_full	Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy
title_fullStr	Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy
title_full_unstemmed	Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy
title_sort	dicer-mediated mirna processing is not involved in controlling muscle mass during muscle atrophy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bf7bca035e6e4290ba7afcaaf3ebffc5
work_keys_str_mv	AT satoshioikawa dicermediatedmirnaprocessingisnotinvolvedincontrollingmusclemassduringmuscleatrophy AT jaehoonshin dicermediatedmirnaprocessingisnotinvolvedincontrollingmusclemassduringmuscleatrophy AT takaoakama dicermediatedmirnaprocessingisnotinvolvedincontrollingmusclemassduringmuscleatrophy AT takayukiakimoto dicermediatedmirnaprocessingisnotinvolvedincontrollingmusclemassduringmuscleatrophy
_version_	1718376934933528576

Dicer-mediated miRNA processing is not involved in controlling muscle mass during muscle atrophy

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