Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans

Anna Claudia Mombrini Silva Barbosa,1 Laryssa Pinheiro Costa Silva,1,2 Carolina Magri Ferraz,1 Fernando Luiz Tobias,1 Jackson Victor de Araújo,3 Barbara Loureiro,1 Gracilene Maria Almeida Muniz Braga,4 Francielle Bosi Rodrigues Veloso,4 Filippe Elias de Freitas Soares,3 Marcio Fronza,4 Fa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Barbosa ACMS, Silva LPC, Ferraz CM, Tobias FL, de Araújo JV, Loureiro B, Braga GMAM, Veloso FBR, Soares FEF, Fronza M, Braga FR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Nanotechnology biomedicine nanoparticles helminths biological control Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bf7f0f8d9e2f4eae97b6dc036a9b6488
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bf7f0f8d9e2f4eae97b6dc036a9b6488
record_format	dspace
spelling	oai:doaj.org-article:bf7f0f8d9e2f4eae97b6dc036a9b64882021-12-02T10:04:21ZNematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans1178-2013https://doaj.org/article/bf7f0f8d9e2f4eae97b6dc036a9b64882019-04-01T00:00:00Zhttps://www.dovepress.com/nematicidal-activity-of-silver-nanoparticles-from-the-fungus-duddingto-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Anna Claudia Mombrini Silva Barbosa,1 Laryssa Pinheiro Costa Silva,1,2 Carolina Magri Ferraz,1 Fernando Luiz Tobias,1 Jackson Victor de Araújo,3 Barbara Loureiro,1 Gracilene Maria Almeida Muniz Braga,4 Francielle Bosi Rodrigues Veloso,4 Filippe Elias de Freitas Soares,3 Marcio Fronza,4 Fabio Ribeiro Braga1 1Parasitology and Biological Control Laboratory, Universidade Vila Velha, Vila Velha, Brazil; 2Morphology Department, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil; 3Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Brazil; 4Department of Pharmaceutical Sciences, Universidade Vila Velha, Brazil Background: Helminth parasites cause morbidity and mortality in both humans and animals. Most anthelmintic drugs used in the treatment of parasitic nematode infections act on target proteins or regulate the electrical activity of neurons and muscles. In this way, it can lead to paralysis, starvation, immune attack, and expulsion of the worm. However, current anthelmintics have some limitations that include a limited spectrum of activity across species and the threat of drug resistance, which highlights the need for new drugs for human and veterinary medicine.Purpose: Present study has been conducted to determine the anthelmintic activity of silver nanoparticles (AgNPs) synthesized from the extract of nematophagous fungus, Duddingtonia flagrans, on the infecting larvae of Ancylostoma caninum (L3). Methods: The nanoparticles were characterized by visual, ultraviolet, Fourier-transform infrared spectroscopy, transmission electron microscopy (TEM) analysis, and X-ray diffraction. The in vitro study was based on experiments to inhibit the motility of infective larvae (L3), and the ultrastructural analysis of the nematode was performed by images obtained by TEM. Results: The XRD studies revealed the crystalline nature of the nanoparticles, and FTIR results implied that AgNPs were successfully synthesized and capped with compounds present in the extract. The results showed that the green synthesis of AgNPs exhibited nematicidal activity, being the only ones capable of penetrating the cuticle of the larvae, causing changes in the tegmentum, and consequently, the death of the nematode. Conclusion: The extract of the fungus D. flagrans is able to synthesize AgNP and these have a nematicidal action. Keywords: nanotechnology, biomedicine, nanoparticles, helminths, biological controlBarbosa ACMSSilva LPCFerraz CMTobias FLde Araújo JVLoureiro BBraga GMAMVeloso FBRSoares FEFFronza MBraga FRDove Medical PressarticleNanotechnologybiomedicinenanoparticleshelminthsbiological controlMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2341-2348 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanotechnology biomedicine nanoparticles helminths biological control Medicine (General) R5-920
spellingShingle	Nanotechnology biomedicine nanoparticles helminths biological control Medicine (General) R5-920 Barbosa ACMS Silva LPC Ferraz CM Tobias FL de Araújo JV Loureiro B Braga GMAM Veloso FBR Soares FEF Fronza M Braga FR Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans
description	Anna Claudia Mombrini Silva Barbosa,1 Laryssa Pinheiro Costa Silva,1,2 Carolina Magri Ferraz,1 Fernando Luiz Tobias,1 Jackson Victor de Araújo,3 Barbara Loureiro,1 Gracilene Maria Almeida Muniz Braga,4 Francielle Bosi Rodrigues Veloso,4 Filippe Elias de Freitas Soares,3 Marcio Fronza,4 Fabio Ribeiro Braga1 1Parasitology and Biological Control Laboratory, Universidade Vila Velha, Vila Velha, Brazil; 2Morphology Department, Federal University of Espírito Santo, Vitória, Espírito Santo, Brazil; 3Department of Veterinary Medicine, Universidade Federal de Viçosa, Viçosa, Brazil; 4Department of Pharmaceutical Sciences, Universidade Vila Velha, Brazil Background: Helminth parasites cause morbidity and mortality in both humans and animals. Most anthelmintic drugs used in the treatment of parasitic nematode infections act on target proteins or regulate the electrical activity of neurons and muscles. In this way, it can lead to paralysis, starvation, immune attack, and expulsion of the worm. However, current anthelmintics have some limitations that include a limited spectrum of activity across species and the threat of drug resistance, which highlights the need for new drugs for human and veterinary medicine.Purpose: Present study has been conducted to determine the anthelmintic activity of silver nanoparticles (AgNPs) synthesized from the extract of nematophagous fungus, Duddingtonia flagrans, on the infecting larvae of Ancylostoma caninum (L3). Methods: The nanoparticles were characterized by visual, ultraviolet, Fourier-transform infrared spectroscopy, transmission electron microscopy (TEM) analysis, and X-ray diffraction. The in vitro study was based on experiments to inhibit the motility of infective larvae (L3), and the ultrastructural analysis of the nematode was performed by images obtained by TEM. Results: The XRD studies revealed the crystalline nature of the nanoparticles, and FTIR results implied that AgNPs were successfully synthesized and capped with compounds present in the extract. The results showed that the green synthesis of AgNPs exhibited nematicidal activity, being the only ones capable of penetrating the cuticle of the larvae, causing changes in the tegmentum, and consequently, the death of the nematode. Conclusion: The extract of the fungus D. flagrans is able to synthesize AgNP and these have a nematicidal action. Keywords: nanotechnology, biomedicine, nanoparticles, helminths, biological control
format	article
author	Barbosa ACMS Silva LPC Ferraz CM Tobias FL de Araújo JV Loureiro B Braga GMAM Veloso FBR Soares FEF Fronza M Braga FR
author_facet	Barbosa ACMS Silva LPC Ferraz CM Tobias FL de Araújo JV Loureiro B Braga GMAM Veloso FBR Soares FEF Fronza M Braga FR
author_sort	Barbosa ACMS
title	Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans
title_short	Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans
title_full	Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans
title_fullStr	Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans
title_full_unstemmed	Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans
title_sort	nematicidal activity of silver nanoparticles from the fungus duddingtonia flagrans
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/bf7f0f8d9e2f4eae97b6dc036a9b6488
work_keys_str_mv	AT barbosaacms nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT silvalpc nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT ferrazcm nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT tobiasfl nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT dearaujojv nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT loureirob nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT bragagmam nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT velosofbr nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT soaresfef nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT fronzam nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans AT bragafr nematicidalactivityofsilvernanoparticlesfromthefungusduddingtoniaflagrans
_version_	1718397704619425792

Nematicidal activity of silver nanoparticles from the fungus Duddingtonia flagrans

Ejemplares similares