The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

Xinchao Miao,1,2,* Donghui Wang,3,4,* Lianyi Xu,1,2 Jie Wang,1,2 Deliang Zeng,1,2 Shuxian Lin,1,2 Cui Huang,5 Xuanyong Liu,3 Xinquan Jiang1,2 1Department of Prosthodontics, 2Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People’s Hospital Affiliated to Shanghai...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Miao X, Wang D, Xu L, Wang J, Zeng D, Lin S, Huang C, Liu X, Jiang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	nanotopography osteoblast gingival epithelial cells gingival fibroblasts macrophage polarization oral bacteria Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bf87849f1945485e8866d964ed9a2fc8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bf87849f1945485e8866d964ed9a2fc8
record_format	dspace
spelling	oai:doaj.org-article:bf87849f1945485e8866d964ed9a2fc82021-12-02T07:28:29ZThe response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study1178-2013https://doaj.org/article/bf87849f1945485e8866d964ed9a2fc82017-02-01T00:00:00Zhttps://www.dovepress.com/the-response-of-human-osteoblasts-epithelial-cells-fibroblasts-macroph-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xinchao Miao,1,2,* Donghui Wang,3,4,* Lianyi Xu,1,2 Jie Wang,1,2 Deliang Zeng,1,2 Shuxian Lin,1,2 Cui Huang,5 Xuanyong Liu,3 Xinquan Jiang1,2 1Department of Prosthodontics, 2Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, 3State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 4University of Chinese Academy of Sciences, Beijing, 5The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, People’s Republic of China*These authors contributed equally to this workAbstract: Nanotopography modification is a major focus of interest in current titanium surface design; however, the influence of the nanostructured surface on human cell/bacterium behavior has rarely been systematically evaluated. In this study, a homogeneous nanofiber structure was prepared on a titanium surface (Nano) by alkali-hydrothermal treatment, and the effects of this Nano surface on the behaviors of human MG-63 osteoblasts, human gingival epithelial cells (HGECs) and human gingival fibroblasts (HGFs) were evaluated in comparison with a smooth titanium surface (Smooth) by polishing and a micro-rough titanium surface (Micro) by sandblasting and acid etching. In addition, the impacts of these different surface morphologies on human THP-1 macrophage polarization and Streptococcus mutans attachment were also assessed. Our findings showed that the nanostructured surface enhanced the osteogenic activity of MG-63 cells (Nano=Micro>Smooth) at the same time that it improved the attachment of HGECs (Nano>Smooth>Micro) and HGFs (Nano=Micro>Smooth). Furthermore, the surface with nanotexture did not affect macrophage polarization (Nano=Micro=Smooth), but did reduce initial bacterial adhesion (Nano<Smooth<Micro). These results suggest that the nanostructured titanium surface may promote bone and soft tissue healing, and thereby increase the success and survival of dental implants.Keywords: nanotopography, osteogenic differentiation, soft tissue barrier, macrophage polarization, antimicrobial effectMiao XWang DXu LWang JZeng DLin SHuang CLiu XJiang XDove Medical Pressarticlenanotopographyosteoblastgingival epithelial cellsgingival fibroblastsmacrophage polarizationoral bacteriaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1415-1430 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanotopography osteoblast gingival epithelial cells gingival fibroblasts macrophage polarization oral bacteria Medicine (General) R5-920
spellingShingle	nanotopography osteoblast gingival epithelial cells gingival fibroblasts macrophage polarization oral bacteria Medicine (General) R5-920 Miao X Wang D Xu L Wang J Zeng D Lin S Huang C Liu X Jiang X The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study
description	Xinchao Miao,1,2,* Donghui Wang,3,4,* Lianyi Xu,1,2 Jie Wang,1,2 Deliang Zeng,1,2 Shuxian Lin,1,2 Cui Huang,5 Xuanyong Liu,3 Xinquan Jiang1,2 1Department of Prosthodontics, 2Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, 3State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 4University of Chinese Academy of Sciences, Beijing, 5The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM), School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, People’s Republic of China*These authors contributed equally to this workAbstract: Nanotopography modification is a major focus of interest in current titanium surface design; however, the influence of the nanostructured surface on human cell/bacterium behavior has rarely been systematically evaluated. In this study, a homogeneous nanofiber structure was prepared on a titanium surface (Nano) by alkali-hydrothermal treatment, and the effects of this Nano surface on the behaviors of human MG-63 osteoblasts, human gingival epithelial cells (HGECs) and human gingival fibroblasts (HGFs) were evaluated in comparison with a smooth titanium surface (Smooth) by polishing and a micro-rough titanium surface (Micro) by sandblasting and acid etching. In addition, the impacts of these different surface morphologies on human THP-1 macrophage polarization and Streptococcus mutans attachment were also assessed. Our findings showed that the nanostructured surface enhanced the osteogenic activity of MG-63 cells (Nano=Micro>Smooth) at the same time that it improved the attachment of HGECs (Nano>Smooth>Micro) and HGFs (Nano=Micro>Smooth). Furthermore, the surface with nanotexture did not affect macrophage polarization (Nano=Micro=Smooth), but did reduce initial bacterial adhesion (Nano<Smooth<Micro). These results suggest that the nanostructured titanium surface may promote bone and soft tissue healing, and thereby increase the success and survival of dental implants.Keywords: nanotopography, osteogenic differentiation, soft tissue barrier, macrophage polarization, antimicrobial effect
format	article
author	Miao X Wang D Xu L Wang J Zeng D Lin S Huang C Liu X Jiang X
author_facet	Miao X Wang D Xu L Wang J Zeng D Lin S Huang C Liu X Jiang X
author_sort	Miao X
title	The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study
title_short	The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study
title_full	The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study
title_fullStr	The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study
title_full_unstemmed	The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study
title_sort	response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/bf87849f1945485e8866d964ed9a2fc8
work_keys_str_mv	AT miaox theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT wangd theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT xul theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT wangj theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT zengd theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT lins theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT huangc theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT liux theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT jiangx theresponseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT miaox responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT wangd responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT xul responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT wangj responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT zengd responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT lins responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT huangc responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT liux responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy AT jiangx responseofhumanosteoblastsepithelialcellsfibroblastsmacrophagesandoralbacteriatonanostructuredtitaniumsurfacesasystematicstudy
_version_	1718399422256119808

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

Ejemplares similares