The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis)
Polyphenol oxidase (PPO) plays a role in stress response, secondary metabolism, and other physiological processes during plant growth and development, and is also a critical enzyme in black tea production. However, the regulatory mechanisms of PPO genes and their activity in tea plants are still unc...
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2021
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oai:doaj.org-article:bf89546839ea4bbaac238a778a8664592021-11-04T09:31:31ZThe R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis)1664-462X10.3389/fpls.2021.739951https://doaj.org/article/bf89546839ea4bbaac238a778a8664592021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.739951/fullhttps://doaj.org/toc/1664-462XPolyphenol oxidase (PPO) plays a role in stress response, secondary metabolism, and other physiological processes during plant growth and development, and is also a critical enzyme in black tea production. However, the regulatory mechanisms of PPO genes and their activity in tea plants are still unclear. In this study, we measured PPO activity in two different tea cultivars, Taoyuandaye (TYDY) and Bixiangzao (BXZ), which are commonly used to produce black tea and green tea, respectively. The expression pattern of CsPPO1 was assessed and validated via transcriptomics and quantitative polymerase chain reaction in both tea varieties. In addition, we isolated and identified an R2R3-MYB transcription factor CsMYB59 that may regulate CsPPO1 expression. CsMYB59 was found to be a nuclear protein, and its expression in tea leaves was positively correlated with CsPPO1 expression and PPO activity. Transcriptional activity analysis showed that CsMYB59 was a transcriptional activator, and the dual-luciferase assay indicated that CsMYB59 could activate the expression of CsPPO1 in tobacco leaves. In summary, our study demonstrates that CsMYB59 represents a transcriptional activator in tea plants and may mediate the regulation of PPO activity by activating CsPPO1 expression. These findings provide novel insights into the regulatory mechanism of PPO gene in Camellia sinensis, which might help to breed tea cultivars with high PPO activity.Xiangxiang HuangXiangxiang HuangShuqiong OuShuqiong OuQin LiQin LiYong LuoHaiyan LinHaiyan LinJuan LiJuan LiMingzhi ZhuMingzhi ZhuKunbo WangKunbo WangFrontiers Media S.A.articleCamellia sinensispolyphenol oxidase activitytranscription factorCsMYB59CsPPO1 expressionPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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| topic |
Camellia sinensis polyphenol oxidase activity transcription factor CsMYB59 CsPPO1 expression Plant culture SB1-1110 |
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Camellia sinensis polyphenol oxidase activity transcription factor CsMYB59 CsPPO1 expression Plant culture SB1-1110 Xiangxiang Huang Xiangxiang Huang Shuqiong Ou Shuqiong Ou Qin Li Qin Li Yong Luo Haiyan Lin Haiyan Lin Juan Li Juan Li Mingzhi Zhu Mingzhi Zhu Kunbo Wang Kunbo Wang The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis) |
| description |
Polyphenol oxidase (PPO) plays a role in stress response, secondary metabolism, and other physiological processes during plant growth and development, and is also a critical enzyme in black tea production. However, the regulatory mechanisms of PPO genes and their activity in tea plants are still unclear. In this study, we measured PPO activity in two different tea cultivars, Taoyuandaye (TYDY) and Bixiangzao (BXZ), which are commonly used to produce black tea and green tea, respectively. The expression pattern of CsPPO1 was assessed and validated via transcriptomics and quantitative polymerase chain reaction in both tea varieties. In addition, we isolated and identified an R2R3-MYB transcription factor CsMYB59 that may regulate CsPPO1 expression. CsMYB59 was found to be a nuclear protein, and its expression in tea leaves was positively correlated with CsPPO1 expression and PPO activity. Transcriptional activity analysis showed that CsMYB59 was a transcriptional activator, and the dual-luciferase assay indicated that CsMYB59 could activate the expression of CsPPO1 in tobacco leaves. In summary, our study demonstrates that CsMYB59 represents a transcriptional activator in tea plants and may mediate the regulation of PPO activity by activating CsPPO1 expression. These findings provide novel insights into the regulatory mechanism of PPO gene in Camellia sinensis, which might help to breed tea cultivars with high PPO activity. |
| format |
article |
| author |
Xiangxiang Huang Xiangxiang Huang Shuqiong Ou Shuqiong Ou Qin Li Qin Li Yong Luo Haiyan Lin Haiyan Lin Juan Li Juan Li Mingzhi Zhu Mingzhi Zhu Kunbo Wang Kunbo Wang |
| author_facet |
Xiangxiang Huang Xiangxiang Huang Shuqiong Ou Shuqiong Ou Qin Li Qin Li Yong Luo Haiyan Lin Haiyan Lin Juan Li Juan Li Mingzhi Zhu Mingzhi Zhu Kunbo Wang Kunbo Wang |
| author_sort |
Xiangxiang Huang |
| title |
The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis) |
| title_short |
The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis) |
| title_full |
The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis) |
| title_fullStr |
The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis) |
| title_full_unstemmed |
The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis) |
| title_sort |
r2r3 transcription factor csmyb59 regulates polyphenol oxidase gene csppo1 in tea plants (camellia sinensis) |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/bf89546839ea4bbaac238a778a866459 |
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