Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer

<i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer is an ornamental plant with certain medicinal values from the Orchidaceae family. Currently, micropropagation is the main means through which it is propagated. In this research, the effects of different daytime and nighttime temperatures (...

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Autores principales:	Boling Liu, Lijun Tang, Bowen Gao, Byoung Ryong Jeong, Shijun Deng, Hao Wei
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	abiotic stress bioactive compound enzyme activity polysaccharide plant quality Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/bf8affcd0f094684ae21d1bfc285cfe6
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spelling	oai:doaj.org-article:bf8affcd0f094684ae21d1bfc285cfe62021-11-25T17:46:59ZEffects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer10.3390/horticulturae71104252311-7524https://doaj.org/article/bf8affcd0f094684ae21d1bfc285cfe62021-10-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/425https://doaj.org/toc/2311-7524<i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer is an ornamental plant with certain medicinal values from the Orchidaceae family. Currently, micropropagation is the main means through which it is propagated. In this research, the effects of different daytime and nighttime temperatures (DIF) and medium supplementation with rare earth elements (REE) on the growth and metabolism of <i>D. aphyllum</i> during a micropropagation period were investigated. Three kinds of REE nitrates, La(NO₃)₃, Ce(NO₃)₃ and Nd(NO₃)₃, at four concentrations, 50, 100, 150, and 200 μM, were added to the culture medium. Three DIFs, 0, 6 and 12 °C, with an identical mean daily temperature of 20 °C, were used with photoperiod/dark period temperatures of 20/20 °C, 23/17 °C and 26/14 °C, respectively. After four weeks of culture, both supplemental REE and DIF treatments promoted growth of the plant compared with the control group. With increased REE concentration and DIF, the growth of the plants was suppressed and symptoms of stress response occurred. At the same concentration, Ce(NO₃)₃ had a more significant promotional effect on plant growth. In general, the medium supplemented with 100 μM Ce(NO₃)₃ combined with a 6 °C DIF was the most optimal for the vegetative growth of the plant. A 12 °C DIF promoted synthesis of more primary metabolites such as total proteins and polysaccharides. From the perspective of improving the medicinal values of this plant, increasing the DIF for an optimal growth environment is a valuable reference. This study can provide a technical basis for the propagation and production of <i>Dendrobium</i> <i>aphyllum</i> in the future.Boling LiuLijun TangBowen GaoByoung Ryong JeongShijun DengHao WeiMDPI AGarticleabiotic stressbioactive compoundenzyme activitypolysaccharideplant qualityPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 425, p 425 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	abiotic stress bioactive compound enzyme activity polysaccharide plant quality Plant culture SB1-1110
spellingShingle	abiotic stress bioactive compound enzyme activity polysaccharide plant quality Plant culture SB1-1110 Boling Liu Lijun Tang Bowen Gao Byoung Ryong Jeong Shijun Deng Hao Wei Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer
description	<i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer is an ornamental plant with certain medicinal values from the Orchidaceae family. Currently, micropropagation is the main means through which it is propagated. In this research, the effects of different daytime and nighttime temperatures (DIF) and medium supplementation with rare earth elements (REE) on the growth and metabolism of <i>D. aphyllum</i> during a micropropagation period were investigated. Three kinds of REE nitrates, La(NO₃)₃, Ce(NO₃)₃ and Nd(NO₃)₃, at four concentrations, 50, 100, 150, and 200 μM, were added to the culture medium. Three DIFs, 0, 6 and 12 °C, with an identical mean daily temperature of 20 °C, were used with photoperiod/dark period temperatures of 20/20 °C, 23/17 °C and 26/14 °C, respectively. After four weeks of culture, both supplemental REE and DIF treatments promoted growth of the plant compared with the control group. With increased REE concentration and DIF, the growth of the plants was suppressed and symptoms of stress response occurred. At the same concentration, Ce(NO₃)₃ had a more significant promotional effect on plant growth. In general, the medium supplemented with 100 μM Ce(NO₃)₃ combined with a 6 °C DIF was the most optimal for the vegetative growth of the plant. A 12 °C DIF promoted synthesis of more primary metabolites such as total proteins and polysaccharides. From the perspective of improving the medicinal values of this plant, increasing the DIF for an optimal growth environment is a valuable reference. This study can provide a technical basis for the propagation and production of <i>Dendrobium</i> <i>aphyllum</i> in the future.
format	article
author	Boling Liu Lijun Tang Bowen Gao Byoung Ryong Jeong Shijun Deng Hao Wei
author_facet	Boling Liu Lijun Tang Bowen Gao Byoung Ryong Jeong Shijun Deng Hao Wei
author_sort	Boling Liu
title	Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer
title_short	Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer
title_full	Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer
title_fullStr	Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer
title_full_unstemmed	Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer
title_sort	effects on growth and metabolism of difference between day and night temperatures (dif) and supplementation with rare earth elements (ree) in micropropagated <i>dendrobium aphyllum</i> (roxb.) c. e. fischer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bf8affcd0f094684ae21d1bfc285cfe6
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Effects on Growth and Metabolism of Difference between Day and Night Temperatures (DIF) and Supplementation with Rare Earth Elements (REE) in Micropropagated <i>Dendrobium aphyllum</i> (Roxb.) C. E. Fischer

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