BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.

The long-term decline of managed honeybee hives in the world has drawn significant attention to the scientific community and bee-keeping industry. A high pathogen load is believed to play a crucial role in this phenomenon, with the bee viruses being key players. Most of the currently characterized h...

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Autores principales:	Lina De Smet, Jorgen Ravoet, Joachim R de Miranda, Tom Wenseleers, Matthias Y Mueller, Robin F A Moritz, Dirk C de Graaf
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bf96023a3d4a47d799d38c3c78ee5326
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spelling	oai:doaj.org-article:bf96023a3d4a47d799d38c3c78ee53262021-11-18T08:10:47ZBeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.1932-620310.1371/journal.pone.0047953https://doaj.org/article/bf96023a3d4a47d799d38c3c78ee53262012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144717/?tool=EBIhttps://doaj.org/toc/1932-6203The long-term decline of managed honeybee hives in the world has drawn significant attention to the scientific community and bee-keeping industry. A high pathogen load is believed to play a crucial role in this phenomenon, with the bee viruses being key players. Most of the currently characterized honeybee viruses (around twenty) are positive stranded RNA viruses. Techniques based on RNA signatures are widely used to determine the viral load in honeybee colonies. High throughput screening for viral loads necessitates the development of a multiplex polymerase chain reaction approach in which different viruses can be targeted simultaneously. A new multiparameter assay, called "BeeDoctor", was developed based on multiplex-ligation probe dependent amplification (MLPA) technology. This assay detects 10 honeybee viruses in one reaction. "BeeDoctor" is also able to screen selectively for either the positive strand of the targeted RNA bee viruses or the negative strand, which is indicative for active viral replication. Due to its sensitivity and specificity, the MLPA assay is a useful tool for rapid diagnosis, pathogen characterization, and epidemiology of viruses in honeybee populations. "BeeDoctor" was used for screening 363 samples from apiaries located throughout Flanders; the northern half of Belgium. Using the "BeeDoctor", virus infections were detected in almost eighty percent of the colonies, with deformed wing virus by far the most frequently detected virus and multiple virus infections were found in 26 percent of the colonies.Lina De SmetJorgen RavoetJoachim R de MirandaTom WenseleersMatthias Y MuellerRobin F A MoritzDirk C de GraafPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47953 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lina De Smet Jorgen Ravoet Joachim R de Miranda Tom Wenseleers Matthias Y Mueller Robin F A Moritz Dirk C de Graaf BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.
description	The long-term decline of managed honeybee hives in the world has drawn significant attention to the scientific community and bee-keeping industry. A high pathogen load is believed to play a crucial role in this phenomenon, with the bee viruses being key players. Most of the currently characterized honeybee viruses (around twenty) are positive stranded RNA viruses. Techniques based on RNA signatures are widely used to determine the viral load in honeybee colonies. High throughput screening for viral loads necessitates the development of a multiplex polymerase chain reaction approach in which different viruses can be targeted simultaneously. A new multiparameter assay, called "BeeDoctor", was developed based on multiplex-ligation probe dependent amplification (MLPA) technology. This assay detects 10 honeybee viruses in one reaction. "BeeDoctor" is also able to screen selectively for either the positive strand of the targeted RNA bee viruses or the negative strand, which is indicative for active viral replication. Due to its sensitivity and specificity, the MLPA assay is a useful tool for rapid diagnosis, pathogen characterization, and epidemiology of viruses in honeybee populations. "BeeDoctor" was used for screening 363 samples from apiaries located throughout Flanders; the northern half of Belgium. Using the "BeeDoctor", virus infections were detected in almost eighty percent of the colonies, with deformed wing virus by far the most frequently detected virus and multiple virus infections were found in 26 percent of the colonies.
format	article
author	Lina De Smet Jorgen Ravoet Joachim R de Miranda Tom Wenseleers Matthias Y Mueller Robin F A Moritz Dirk C de Graaf
author_facet	Lina De Smet Jorgen Ravoet Joachim R de Miranda Tom Wenseleers Matthias Y Mueller Robin F A Moritz Dirk C de Graaf
author_sort	Lina De Smet
title	BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.
title_short	BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.
title_full	BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.
title_fullStr	BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.
title_full_unstemmed	BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.
title_sort	beedoctor, a versatile mlpa-based diagnostic tool for screening bee viruses.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/bf96023a3d4a47d799d38c3c78ee5326
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BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.

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