Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells

Simona Mura1,2, Herve Hillaireau1,2, Julien Nicolas1,2, Benjamin Le Droumaguet1,2, Claire Gueutin1,2, Sandrine Zanna3, Nicolas Tsapis1,2, Elias Fattal1,2 1Univ Paris-Sud, UMR 8612, Ch&acirc;tenay Malabry, F-92296; 2CNRS, Ch&acirc;tenay Malabry, F-92296; 3Laboratoire de Physico-Chimie...

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Autores principales:	Mura S, Hillaireau H, Nicolas J, Le Droumaguet B, Gueutin C, Zanna S, Tsapis N, Fattal E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/bf98dcb37601409c96eb57546fb2ce02
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spelling	oai:doaj.org-article:bf98dcb37601409c96eb57546fb2ce022021-12-02T05:11:30ZInfluence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells1176-91141178-2013https://doaj.org/article/bf98dcb37601409c96eb57546fb2ce022011-10-01T00:00:00Zhttp://www.dovepress.com/influence-of-surface-charge-on-the-potential-toxicity-of-plga-nanopart-a8544https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Simona Mura1,2, Herve Hillaireau1,2, Julien Nicolas1,2, Benjamin Le Droumaguet1,2, Claire Gueutin1,2, Sandrine Zanna3, Nicolas Tsapis1,2, Elias Fattal1,2 1Univ Paris-Sud, UMR 8612, Ch&acirc;tenay Malabry, F-92296; 2CNRS, Ch&acirc;tenay Malabry, F-92296; 3Laboratoire de Physico-Chimie des Surfaces, CNRS-ENSCP (UMR 7045), Ecole Nationale Superiore de Chimie de Paris, France Background: Because of the described hazards related to inhalation of manufactured nanoparticles, we investigated the lung toxicity of biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticles displaying various surface properties on human bronchial Calu-3 cells. Methods: Positively and negatively charged as well as neutral nanoparticles were tailored by coating their surface with chitosan, Poloxamer, or poly (vinyl alcohol), respectively. Nanoparticles were characterized in terms of size, zeta potential, and surface chemical composition, confirming modifications provided by hydrophilic polymers. Results: Although nanoparticle internalization by lung cells was clearly demonstrated, the cytotoxicity of the nanoparticles was very limited, with an absence of inflammatory response, regardless of the surface properties of the PLGA nanoparticles. Conclusion: These in vitro results highlight the safety of biodegradable PLGA nanoparticles in the bronchial epithelium and provide initial data on their potential effects and the risks associated with their use as nanomedicines. Keywords: nanoparticles, PLGA, surface properties, Calu-3, toxicity, inflammationMura SHillaireau HNicolas JLe Droumaguet BGueutin CZanna STsapis NFattal EDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2591-2605 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Mura S Hillaireau H Nicolas J Le Droumaguet B Gueutin C Zanna S Tsapis N Fattal E Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells
description	Simona Mura1,2, Herve Hillaireau1,2, Julien Nicolas1,2, Benjamin Le Droumaguet1,2, Claire Gueutin1,2, Sandrine Zanna3, Nicolas Tsapis1,2, Elias Fattal1,2 1Univ Paris-Sud, UMR 8612, Ch&acirc;tenay Malabry, F-92296; 2CNRS, Ch&acirc;tenay Malabry, F-92296; 3Laboratoire de Physico-Chimie des Surfaces, CNRS-ENSCP (UMR 7045), Ecole Nationale Superiore de Chimie de Paris, France Background: Because of the described hazards related to inhalation of manufactured nanoparticles, we investigated the lung toxicity of biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticles displaying various surface properties on human bronchial Calu-3 cells. Methods: Positively and negatively charged as well as neutral nanoparticles were tailored by coating their surface with chitosan, Poloxamer, or poly (vinyl alcohol), respectively. Nanoparticles were characterized in terms of size, zeta potential, and surface chemical composition, confirming modifications provided by hydrophilic polymers. Results: Although nanoparticle internalization by lung cells was clearly demonstrated, the cytotoxicity of the nanoparticles was very limited, with an absence of inflammatory response, regardless of the surface properties of the PLGA nanoparticles. Conclusion: These in vitro results highlight the safety of biodegradable PLGA nanoparticles in the bronchial epithelium and provide initial data on their potential effects and the risks associated with their use as nanomedicines. Keywords: nanoparticles, PLGA, surface properties, Calu-3, toxicity, inflammation
format	article
author	Mura S Hillaireau H Nicolas J Le Droumaguet B Gueutin C Zanna S Tsapis N Fattal E
author_facet	Mura S Hillaireau H Nicolas J Le Droumaguet B Gueutin C Zanna S Tsapis N Fattal E
author_sort	Mura S
title	Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells
title_short	Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells
title_full	Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells
title_fullStr	Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells
title_full_unstemmed	Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells
title_sort	influence of surface charge on the potential toxicity of plga nanoparticles towards calu-3 cells
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/bf98dcb37601409c96eb57546fb2ce02
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Influence of surface charge on the potential toxicity of PLGA nanoparticles towards Calu-3 cells

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