Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology

Nosocomial infections are a major public health problem. They are mainly caused by bacteria that often present antibiotic resistance profiles, which complicates their management. The diagnosis of these infections is based on clinical and biological criteria that lack sensitivity and specificity, and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lahdibi Sahraoui Hajar, Qasmaoui Aicha, Charof Réda, Hamamouchi Jamila, Berny El Hassan
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	nosocomial infections esbl pcr resistance genes surveillance Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/bf9d3c63ae144aaea825db02ffe433e1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:bf9d3c63ae144aaea825db02ffe433e1
record_format	dspace
spelling	oai:doaj.org-article:bf9d3c63ae144aaea825db02ffe433e12021-11-12T11:44:08ZNosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology2267-124210.1051/e3sconf/202131901014https://doaj.org/article/bf9d3c63ae144aaea825db02ffe433e12021-01-01T00:00:00Zhttps://www.e3s-conferences.org/articles/e3sconf/pdf/2021/95/e3sconf_vigisan_01014.pdfhttps://doaj.org/toc/2267-1242Nosocomial infections are a major public health problem. They are mainly caused by bacteria that often present antibiotic resistance profiles, which complicates their management. The diagnosis of these infections is based on clinical and biological criteria that lack sensitivity and specificity, and on microbiological examinations that are lengthy. Unfortunately, the inadequate empirical antibiotic therapy in many cases, and the late implementation of an effective treatment, are associated with the emergence and spread of bacteria that are multi-resistant to antibiotics, i.e. strains producing extended-spectrum beta-lactamases (ESBL). Rapid diagnostic methods, identifying the pathogen and its resistance profile, are therefore necessary. Our laboratory is committed to develop PCR techniques to genotypically identify the most frequent ESBLs from four hospital facilities. Among all the enterobacteria detected, we observed the predominance of OXA type ESBL (38%), followed by CTX-M type ESBL (33%). TEM and SHV genes represent respectively 19% and 10%.The optimization of these technologies could allow the identification of all known resistance mechanisms in only a few hours and find new preventive or curative strategies in the fight against these nosocomial infections, to better respond to this health threat.Lahdibi Sahraoui HajarQasmaoui AichaCharof RédaHamamouchi JamilaBerny El HassanEDP Sciencesarticlenosocomial infectionsesblpcrresistance genessurveillanceEnvironmental sciencesGE1-350ENFRE3S Web of Conferences, Vol 319, p 01014 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	nosocomial infections esbl pcr resistance genes surveillance Environmental sciences GE1-350
spellingShingle	nosocomial infections esbl pcr resistance genes surveillance Environmental sciences GE1-350 Lahdibi Sahraoui Hajar Qasmaoui Aicha Charof Réda Hamamouchi Jamila Berny El Hassan Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology
description	Nosocomial infections are a major public health problem. They are mainly caused by bacteria that often present antibiotic resistance profiles, which complicates their management. The diagnosis of these infections is based on clinical and biological criteria that lack sensitivity and specificity, and on microbiological examinations that are lengthy. Unfortunately, the inadequate empirical antibiotic therapy in many cases, and the late implementation of an effective treatment, are associated with the emergence and spread of bacteria that are multi-resistant to antibiotics, i.e. strains producing extended-spectrum beta-lactamases (ESBL). Rapid diagnostic methods, identifying the pathogen and its resistance profile, are therefore necessary. Our laboratory is committed to develop PCR techniques to genotypically identify the most frequent ESBLs from four hospital facilities. Among all the enterobacteria detected, we observed the predominance of OXA type ESBL (38%), followed by CTX-M type ESBL (33%). TEM and SHV genes represent respectively 19% and 10%.The optimization of these technologies could allow the identification of all known resistance mechanisms in only a few hours and find new preventive or curative strategies in the fight against these nosocomial infections, to better respond to this health threat.
format	article
author	Lahdibi Sahraoui Hajar Qasmaoui Aicha Charof Réda Hamamouchi Jamila Berny El Hassan
author_facet	Lahdibi Sahraoui Hajar Qasmaoui Aicha Charof Réda Hamamouchi Jamila Berny El Hassan
author_sort	Lahdibi Sahraoui Hajar
title	Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology
title_short	Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology
title_full	Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology
title_fullStr	Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology
title_full_unstemmed	Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology
title_sort	nosocomial infections: importance of rapid and early detection of esbl enterobacteriaceae by molecular biology
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/bf9d3c63ae144aaea825db02ffe433e1
work_keys_str_mv	AT lahdibisahraouihajar nosocomialinfectionsimportanceofrapidandearlydetectionofesblenterobacteriaceaebymolecularbiology AT qasmaouiaicha nosocomialinfectionsimportanceofrapidandearlydetectionofesblenterobacteriaceaebymolecularbiology AT charofreda nosocomialinfectionsimportanceofrapidandearlydetectionofesblenterobacteriaceaebymolecularbiology AT hamamouchijamila nosocomialinfectionsimportanceofrapidandearlydetectionofesblenterobacteriaceaebymolecularbiology AT bernyelhassan nosocomialinfectionsimportanceofrapidandearlydetectionofesblenterobacteriaceaebymolecularbiology
_version_	1718430642264342528

Nosocomial infections: Importance of rapid and early detection of ESBL Enterobacteriaceae by molecular biology

Ejemplares similares