Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.

Penicillium marneffei, the pathogenic thermal dimorphic fungus is a causative agent of a fatal systemic disease, penicilliosis marneffei, in immunocompromised patients especially HIV patients. For growth and survival, this fungus has to adapt to environmental stresses outside and inside host cells a...

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Autores principales:	Panjaphorn Nimmanee, Patrick C Y Woo, Pramote Vanittanakom, Sirida Youngchim, Nongnuch Vanittanakom
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bfa1e4aac3684022a1b67070e834f423
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spelling	oai:doaj.org-article:bfa1e4aac3684022a1b67070e834f4232021-11-25T05:54:50ZFunctional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.1932-620310.1371/journal.pone.0111200https://doaj.org/article/bfa1e4aac3684022a1b67070e834f4232014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0111200https://doaj.org/toc/1932-6203Penicillium marneffei, the pathogenic thermal dimorphic fungus is a causative agent of a fatal systemic disease, penicilliosis marneffei, in immunocompromised patients especially HIV patients. For growth and survival, this fungus has to adapt to environmental stresses outside and inside host cells and this adaptation requires stress signaling pathways and regulation of gene expression under various kinds of stresses. In this report, P. marneffei activating transcription factor (atfA) gene encoding bZip-type transcription factor was characterized. To determine functions of this gene, atfA isogenic mutant strain was constructed using the modified split marker recombination method. The phenotypes and susceptibility to varieties of stresses including osmotic, oxidative, heat, UV, cell wall and cell membrane stresses of the mutant strain were compared with the wild type and the atfA complemented strains. Results demonstrated that the mRNA expression level of P. marneffei atfA gene increased under heat stress at 42°C. The atfA mutant was more sensitive to sodium dodecyl sulphate, amphotericin B and tert-butyl hydroperoxide than the wild type and complemented strains but not hydrogen peroxide, menadione, NaCl, sorbitol, calcofluor white, itraconazole, UV stresses and heat stress at 39°C. In addition, recovery of atfA mutant conidia after mouse and human macrophage infections was significantly decreased compared to those of wild type and complemented strains. These results indicated that the atfA gene was required by P. marneffei under specific stress conditions and might be necessary for fighting against host immune cells during the initiation of infection.Panjaphorn NimmaneePatrick C Y WooPramote VanittanakomSirida YoungchimNongnuch VanittanakomPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 11, p e111200 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Panjaphorn Nimmanee Patrick C Y Woo Pramote Vanittanakom Sirida Youngchim Nongnuch Vanittanakom Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.
description	Penicillium marneffei, the pathogenic thermal dimorphic fungus is a causative agent of a fatal systemic disease, penicilliosis marneffei, in immunocompromised patients especially HIV patients. For growth and survival, this fungus has to adapt to environmental stresses outside and inside host cells and this adaptation requires stress signaling pathways and regulation of gene expression under various kinds of stresses. In this report, P. marneffei activating transcription factor (atfA) gene encoding bZip-type transcription factor was characterized. To determine functions of this gene, atfA isogenic mutant strain was constructed using the modified split marker recombination method. The phenotypes and susceptibility to varieties of stresses including osmotic, oxidative, heat, UV, cell wall and cell membrane stresses of the mutant strain were compared with the wild type and the atfA complemented strains. Results demonstrated that the mRNA expression level of P. marneffei atfA gene increased under heat stress at 42°C. The atfA mutant was more sensitive to sodium dodecyl sulphate, amphotericin B and tert-butyl hydroperoxide than the wild type and complemented strains but not hydrogen peroxide, menadione, NaCl, sorbitol, calcofluor white, itraconazole, UV stresses and heat stress at 39°C. In addition, recovery of atfA mutant conidia after mouse and human macrophage infections was significantly decreased compared to those of wild type and complemented strains. These results indicated that the atfA gene was required by P. marneffei under specific stress conditions and might be necessary for fighting against host immune cells during the initiation of infection.
format	article
author	Panjaphorn Nimmanee Patrick C Y Woo Pramote Vanittanakom Sirida Youngchim Nongnuch Vanittanakom
author_facet	Panjaphorn Nimmanee Patrick C Y Woo Pramote Vanittanakom Sirida Youngchim Nongnuch Vanittanakom
author_sort	Panjaphorn Nimmanee
title	Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.
title_short	Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.
title_full	Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.
title_fullStr	Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.
title_full_unstemmed	Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.
title_sort	functional analysis of atfa gene to stress response in pathogenic thermal dimorphic fungus penicillium marneffei.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/bfa1e4aac3684022a1b67070e834f423
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Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.

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