A novel actin mRNA splice variant regulates ACTG1 expression.

Cytoplasmic actins are abundant, ubiquitous proteins in nucleated cells. However, actin expression is regulated in a tissue- and development-specific manner. We identified a novel cytoplasmic-γ-actin (Actg1) transcript that includes a previously unidentified exon (3a). Inclusion of this exon introdu...

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Autores principales:	Meghan C Drummond, Karen H Friderici
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/bfa2306ca844495eb6c90789fcf69a4f
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spelling	oai:doaj.org-article:bfa2306ca844495eb6c90789fcf69a4f2021-11-18T06:21:44ZA novel actin mRNA splice variant regulates ACTG1 expression.1553-73901553-740410.1371/journal.pgen.1003743https://doaj.org/article/bfa2306ca844495eb6c90789fcf69a4f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098136/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Cytoplasmic actins are abundant, ubiquitous proteins in nucleated cells. However, actin expression is regulated in a tissue- and development-specific manner. We identified a novel cytoplasmic-γ-actin (Actg1) transcript that includes a previously unidentified exon (3a). Inclusion of this exon introduces an in-frame termination codon. We hypothesized this alternatively-spliced transcript down-regulates γ-actin production by targeting these transcripts for nonsense-mediated decay (NMD). To address this, we investigated conservation between mammals, tissue-specificity in mice, and developmental regulation using C2C12 cell culture. Exon 3a is 80% similar among mammals and varies in length from 41 nucleotides in humans to 45 in mice. Though the predicted amino acid sequences are not similar between all species, inclusion of exon 3a consistently results in the in the introduction of a premature termination codon within the alternative Actg1 transcript. Of twelve tissues examined, exon 3a is predominantly expressed in skeletal muscle, cardiac muscle, and diaphragm. Splicing to include exon 3a is concomitant with previously described down-regulation of Actg1 in differentiating C2C12 cells. Treatment of differentiated C2C12 cells with an inhibitor of NMD results in a 7-fold increase in exon 3a-containing transcripts. Therefore, splicing to generate exon 3a-containing transcripts may be one component of Actg1 regulation. We propose that this post-transcriptional regulation occurs via NMD, in a process previously described as "regulated unproductive splicing and translation" (RUST).Meghan C DrummondKaren H FridericiPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 9, Iss 10, p e1003743 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Meghan C Drummond Karen H Friderici A novel actin mRNA splice variant regulates ACTG1 expression.
description	Cytoplasmic actins are abundant, ubiquitous proteins in nucleated cells. However, actin expression is regulated in a tissue- and development-specific manner. We identified a novel cytoplasmic-γ-actin (Actg1) transcript that includes a previously unidentified exon (3a). Inclusion of this exon introduces an in-frame termination codon. We hypothesized this alternatively-spliced transcript down-regulates γ-actin production by targeting these transcripts for nonsense-mediated decay (NMD). To address this, we investigated conservation between mammals, tissue-specificity in mice, and developmental regulation using C2C12 cell culture. Exon 3a is 80% similar among mammals and varies in length from 41 nucleotides in humans to 45 in mice. Though the predicted amino acid sequences are not similar between all species, inclusion of exon 3a consistently results in the in the introduction of a premature termination codon within the alternative Actg1 transcript. Of twelve tissues examined, exon 3a is predominantly expressed in skeletal muscle, cardiac muscle, and diaphragm. Splicing to include exon 3a is concomitant with previously described down-regulation of Actg1 in differentiating C2C12 cells. Treatment of differentiated C2C12 cells with an inhibitor of NMD results in a 7-fold increase in exon 3a-containing transcripts. Therefore, splicing to generate exon 3a-containing transcripts may be one component of Actg1 regulation. We propose that this post-transcriptional regulation occurs via NMD, in a process previously described as "regulated unproductive splicing and translation" (RUST).
format	article
author	Meghan C Drummond Karen H Friderici
author_facet	Meghan C Drummond Karen H Friderici
author_sort	Meghan C Drummond
title	A novel actin mRNA splice variant regulates ACTG1 expression.
title_short	A novel actin mRNA splice variant regulates ACTG1 expression.
title_full	A novel actin mRNA splice variant regulates ACTG1 expression.
title_fullStr	A novel actin mRNA splice variant regulates ACTG1 expression.
title_full_unstemmed	A novel actin mRNA splice variant regulates ACTG1 expression.
title_sort	novel actin mrna splice variant regulates actg1 expression.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bfa2306ca844495eb6c90789fcf69a4f
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_version_	1718424504205574144

A novel actin mRNA splice variant regulates ACTG1 expression.

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