Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon

Abstract Halophilic proteins subjected to below about 15% salt in vitro denature through misfolding, aggregation and/or precipitation. Halobacteria, however, have been detected in environments of fluctuating salinity such as coastal salterns and even around fresh water springs in the depths of the D...

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Autores principales:	P. Vauclare, F. Natali, J. P. Kleman, G. Zaccai, B. Franzetti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bfab62bf0553469084df20621d6bd71a
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spelling	oai:doaj.org-article:bfab62bf0553469084df20621d6bd71a2021-12-02T10:59:52ZSurviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon10.1038/s41598-020-59681-12045-2322https://doaj.org/article/bfab62bf0553469084df20621d6bd71a2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-59681-1https://doaj.org/toc/2045-2322Abstract Halophilic proteins subjected to below about 15% salt in vitro denature through misfolding, aggregation and/or precipitation. Halobacteria, however, have been detected in environments of fluctuating salinity such as coastal salterns and even around fresh water springs in the depths of the Dead Sea. In order to identify the underlying mechanisms of low salt survival, we explored the reactivation capacity of Halobacterium (Hbt) salinarum sub-populations after incubation in low salt media and recovery in physiological salt. Respiratory oxygen consumption was assessed in stressed cells and cell viability was estimated by Live/Dead staining and flow cytometry. In vivo neutron scattering experiments showed that the recovery of Hbt salinarum sub-populations exposed to severe low salt conditions is related to a rapid retrieval of functional molecular dynamics in the proteome. In the hypothesis that the observations on Hbt salinarum have wider relevance, they could be of key ecological significance for the dispersion of extremophiles when environmental fluctuations become severe.P. VauclareF. NataliJ. P. KlemanG. ZaccaiB. FranzettiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P. Vauclare F. Natali J. P. Kleman G. Zaccai B. Franzetti Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon
description	Abstract Halophilic proteins subjected to below about 15% salt in vitro denature through misfolding, aggregation and/or precipitation. Halobacteria, however, have been detected in environments of fluctuating salinity such as coastal salterns and even around fresh water springs in the depths of the Dead Sea. In order to identify the underlying mechanisms of low salt survival, we explored the reactivation capacity of Halobacterium (Hbt) salinarum sub-populations after incubation in low salt media and recovery in physiological salt. Respiratory oxygen consumption was assessed in stressed cells and cell viability was estimated by Live/Dead staining and flow cytometry. In vivo neutron scattering experiments showed that the recovery of Hbt salinarum sub-populations exposed to severe low salt conditions is related to a rapid retrieval of functional molecular dynamics in the proteome. In the hypothesis that the observations on Hbt salinarum have wider relevance, they could be of key ecological significance for the dispersion of extremophiles when environmental fluctuations become severe.
format	article
author	P. Vauclare F. Natali J. P. Kleman G. Zaccai B. Franzetti
author_facet	P. Vauclare F. Natali J. P. Kleman G. Zaccai B. Franzetti
author_sort	P. Vauclare
title	Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon
title_short	Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon
title_full	Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon
title_fullStr	Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon
title_full_unstemmed	Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon
title_sort	surviving salt fluctuations: stress and recovery in halobacterium salinarum, an extreme halophilic archaeon
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/bfab62bf0553469084df20621d6bd71a
work_keys_str_mv	AT pvauclare survivingsaltfluctuationsstressandrecoveryinhalobacteriumsalinarumanextremehalophilicarchaeon AT fnatali survivingsaltfluctuationsstressandrecoveryinhalobacteriumsalinarumanextremehalophilicarchaeon AT jpkleman survivingsaltfluctuationsstressandrecoveryinhalobacteriumsalinarumanextremehalophilicarchaeon AT gzaccai survivingsaltfluctuationsstressandrecoveryinhalobacteriumsalinarumanextremehalophilicarchaeon AT bfranzetti survivingsaltfluctuationsstressandrecoveryinhalobacteriumsalinarumanextremehalophilicarchaeon
_version_	1718396376757305344

Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon

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