Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues

Abstract Colorectal cancer (CRC) is a major cancer, and its precise diagnosis is especially important for the development of effective therapeutics. In a series of metabolome analyses, the levels of very long chain fatty acids (VLCFA) were shown to be elevated in CRC tissues, although the endogenous...

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Autores principales:	Kotaro Hama, Yuko Fujiwara, Tamuro Hayama, Tsuyoshi Ozawa, Keijiro Nozawa, Keiji Matsuda, Yojiro Hashiguchi, Kazuaki Yokoyama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bfb250236fb14b6cad3a33c992f59d42
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spelling	oai:doaj.org-article:bfb250236fb14b6cad3a33c992f59d422021-12-02T16:31:17ZVery long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues10.1038/s41598-021-85603-w2045-2322https://doaj.org/article/bfb250236fb14b6cad3a33c992f59d422021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85603-whttps://doaj.org/toc/2045-2322Abstract Colorectal cancer (CRC) is a major cancer, and its precise diagnosis is especially important for the development of effective therapeutics. In a series of metabolome analyses, the levels of very long chain fatty acids (VLCFA) were shown to be elevated in CRC tissues, although the endogenous form of VLCFA has not been fully elucidated. In this study we analyzed the amount of nonesterified fatty acids, acyl-CoA species, phospholipids and neutral lipids such as cholesterylesters using liquid-chromatography–mass spectrometry. Here we showed that VLCFA were accumulated in triacylglycerol (TAG) and nonesterified forms in CRC tissues. The levels of TAG species harboring a VLCFA moiety (VLCFA-TAG) were significantly correlated with that of nonesterified VLCFA. We also showed that the expression level of elongation of very long-chain fatty acids protein 1 (ELOVL1) is increased in CRC tissues, and the inhibition of ELOVL1 decreased the levels of VLCFA-TAG and nonesterified VLCFA in CRC cell lines. Our results suggest that the upregulation of ELOVL1 contributes to the accumulation of VLCFA-TAG and nonesterified VLCFA in CRC tissues.Kotaro HamaYuko FujiwaraTamuro HayamaTsuyoshi OzawaKeijiro NozawaKeiji MatsudaYojiro HashiguchiKazuaki YokoyamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kotaro Hama Yuko Fujiwara Tamuro Hayama Tsuyoshi Ozawa Keijiro Nozawa Keiji Matsuda Yojiro Hashiguchi Kazuaki Yokoyama Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues
description	Abstract Colorectal cancer (CRC) is a major cancer, and its precise diagnosis is especially important for the development of effective therapeutics. In a series of metabolome analyses, the levels of very long chain fatty acids (VLCFA) were shown to be elevated in CRC tissues, although the endogenous form of VLCFA has not been fully elucidated. In this study we analyzed the amount of nonesterified fatty acids, acyl-CoA species, phospholipids and neutral lipids such as cholesterylesters using liquid-chromatography–mass spectrometry. Here we showed that VLCFA were accumulated in triacylglycerol (TAG) and nonesterified forms in CRC tissues. The levels of TAG species harboring a VLCFA moiety (VLCFA-TAG) were significantly correlated with that of nonesterified VLCFA. We also showed that the expression level of elongation of very long-chain fatty acids protein 1 (ELOVL1) is increased in CRC tissues, and the inhibition of ELOVL1 decreased the levels of VLCFA-TAG and nonesterified VLCFA in CRC cell lines. Our results suggest that the upregulation of ELOVL1 contributes to the accumulation of VLCFA-TAG and nonesterified VLCFA in CRC tissues.
format	article
author	Kotaro Hama Yuko Fujiwara Tamuro Hayama Tsuyoshi Ozawa Keijiro Nozawa Keiji Matsuda Yojiro Hashiguchi Kazuaki Yokoyama
author_facet	Kotaro Hama Yuko Fujiwara Tamuro Hayama Tsuyoshi Ozawa Keijiro Nozawa Keiji Matsuda Yojiro Hashiguchi Kazuaki Yokoyama
author_sort	Kotaro Hama
title	Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues
title_short	Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues
title_full	Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues
title_fullStr	Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues
title_full_unstemmed	Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues
title_sort	very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/bfb250236fb14b6cad3a33c992f59d42
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Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues

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