Hyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer
Yanqing Xu,* Yongrong Yao,* Linxiao Wang, Huachao Chen, Ninghua Tan State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, People’s Republic of China*These authors contributed equally to this workCo...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/bfb499b18f8f40d291191dbce4c7fd74 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:bfb499b18f8f40d291191dbce4c7fd74 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:bfb499b18f8f40d291191dbce4c7fd742021-12-02T16:11:39ZHyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer1178-2013https://doaj.org/article/bfb499b18f8f40d291191dbce4c7fd742021-07-01T00:00:00Zhttps://www.dovepress.com/hyaluronic-acid-coated-liposomes-co-delivery-of-natural-cyclic-peptide-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Yanqing Xu,* Yongrong Yao,* Linxiao Wang, Huachao Chen, Ninghua Tan State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ninghua Tan; Huachao ChenState Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, People’s Republic of ChinaTel/Fax +86 25 86185772Email nhtan@cpu.edu.cn; huachao.chen@cpu.edu.cnBackground: Natural cyclopeptide RA-XII, isolated from Rubia yunnanensis, is a promising chemotherapeutic agent for colon cancer. The photosensitizer protoporphyrin-IX attached with triphenylphosphonium (TPP) could possess mitochondria targeting capacity and exert photodynamic therapy (PDT) by inducing oxidizing damage to the mitochondria and cell apoptosis eventually. In this work, pH-sensitive liposomes were constructed to simultaneously deliver RA-XII as a chemotherapeutic drug and modified porphyrin as a mitochondria-targeting photosensitizer to treat colon cancer, and verified its mechanism of action and antitumor therapeutic efficacy.Methods: The colon cancer targeting liposome nanoparticle RA/TPPP-Lip was synthesized using thin film hydration. The therapeutic effect and targeting ability of RA/TPPP-Lip was investigated in vitro. And use HCT116 cell allogeneic subcutaneous transplantation tumor model to investigate the anti-tumor and targeting effects of RA/TPPP-Lip in vivo.Results: RA/TPPP-Lip gained the targeting ability through surface-modified HA to increase the accumulation of RA-XII and TPPP in colon cancer cells. A series of in vitro experimental results showed that TPPP produced cytotoxic ROS under laser irradiation to directly damage cell mitochondria and played a combined role with RA-XII, making RA/TPPP-Lip the best colon cancer cell growth inhibitory effect. Furthermore, in vivo antitumor experiments showed that the RA/TPPP-Lip substantially accumulated at the tumor site and efficiently repressed tumor growth in nude mice.Conclusion: We have successfully designed a new cancer-targeted nanomedicine platform (RA/TPPP-Lip) for the collaborative treatment of colon cancer, which can achieve the targeted continuous release of multiple therapeutic drugs. This work provides a new strategy for precise combination therapy, which may promote the further development of collaborative cancer treatment platforms.Keywords: natural cyclic peptide, liposome, mitochondrial targeted, photosensitizer, combination therapy nanosystem, colon cancerXu YYao YWang LChen HTan NDove Medical Pressarticlenatural cyclic peptideliposomemitochondrial targetedphotosensitizercombination therapy nanosystemcolon cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 4929-4942 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
natural cyclic peptide liposome mitochondrial targeted photosensitizer combination therapy nanosystem colon cancer Medicine (General) R5-920 |
| spellingShingle |
natural cyclic peptide liposome mitochondrial targeted photosensitizer combination therapy nanosystem colon cancer Medicine (General) R5-920 Xu Y Yao Y Wang L Chen H Tan N Hyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer |
| description |
Yanqing Xu,* Yongrong Yao,* Linxiao Wang, Huachao Chen, Ninghua Tan State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ninghua Tan; Huachao ChenState Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, People’s Republic of ChinaTel/Fax +86 25 86185772Email nhtan@cpu.edu.cn; huachao.chen@cpu.edu.cnBackground: Natural cyclopeptide RA-XII, isolated from Rubia yunnanensis, is a promising chemotherapeutic agent for colon cancer. The photosensitizer protoporphyrin-IX attached with triphenylphosphonium (TPP) could possess mitochondria targeting capacity and exert photodynamic therapy (PDT) by inducing oxidizing damage to the mitochondria and cell apoptosis eventually. In this work, pH-sensitive liposomes were constructed to simultaneously deliver RA-XII as a chemotherapeutic drug and modified porphyrin as a mitochondria-targeting photosensitizer to treat colon cancer, and verified its mechanism of action and antitumor therapeutic efficacy.Methods: The colon cancer targeting liposome nanoparticle RA/TPPP-Lip was synthesized using thin film hydration. The therapeutic effect and targeting ability of RA/TPPP-Lip was investigated in vitro. And use HCT116 cell allogeneic subcutaneous transplantation tumor model to investigate the anti-tumor and targeting effects of RA/TPPP-Lip in vivo.Results: RA/TPPP-Lip gained the targeting ability through surface-modified HA to increase the accumulation of RA-XII and TPPP in colon cancer cells. A series of in vitro experimental results showed that TPPP produced cytotoxic ROS under laser irradiation to directly damage cell mitochondria and played a combined role with RA-XII, making RA/TPPP-Lip the best colon cancer cell growth inhibitory effect. Furthermore, in vivo antitumor experiments showed that the RA/TPPP-Lip substantially accumulated at the tumor site and efficiently repressed tumor growth in nude mice.Conclusion: We have successfully designed a new cancer-targeted nanomedicine platform (RA/TPPP-Lip) for the collaborative treatment of colon cancer, which can achieve the targeted continuous release of multiple therapeutic drugs. This work provides a new strategy for precise combination therapy, which may promote the further development of collaborative cancer treatment platforms.Keywords: natural cyclic peptide, liposome, mitochondrial targeted, photosensitizer, combination therapy nanosystem, colon cancer |
| format |
article |
| author |
Xu Y Yao Y Wang L Chen H Tan N |
| author_facet |
Xu Y Yao Y Wang L Chen H Tan N |
| author_sort |
Xu Y |
| title |
Hyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer |
| title_short |
Hyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer |
| title_full |
Hyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer |
| title_fullStr |
Hyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer |
| title_full_unstemmed |
Hyaluronic Acid Coated Liposomes Co-Delivery of Natural Cyclic Peptide RA-XII and Mitochondrial Targeted Photosensitizer for Highly Selective Precise Combined Treatment of Colon Cancer |
| title_sort |
hyaluronic acid coated liposomes co-delivery of natural cyclic peptide ra-xii and mitochondrial targeted photosensitizer for highly selective precise combined treatment of colon cancer |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/bfb499b18f8f40d291191dbce4c7fd74 |
| work_keys_str_mv |
AT xuy hyaluronicacidcoatedliposomescodeliveryofnaturalcyclicpeptideraxiiandmitochondrialtargetedphotosensitizerforhighlyselectiveprecisecombinedtreatmentofcoloncancer AT yaoy hyaluronicacidcoatedliposomescodeliveryofnaturalcyclicpeptideraxiiandmitochondrialtargetedphotosensitizerforhighlyselectiveprecisecombinedtreatmentofcoloncancer AT wangl hyaluronicacidcoatedliposomescodeliveryofnaturalcyclicpeptideraxiiandmitochondrialtargetedphotosensitizerforhighlyselectiveprecisecombinedtreatmentofcoloncancer AT chenh hyaluronicacidcoatedliposomescodeliveryofnaturalcyclicpeptideraxiiandmitochondrialtargetedphotosensitizerforhighlyselectiveprecisecombinedtreatmentofcoloncancer AT tann hyaluronicacidcoatedliposomescodeliveryofnaturalcyclicpeptideraxiiandmitochondrialtargetedphotosensitizerforhighlyselectiveprecisecombinedtreatmentofcoloncancer |
| _version_ |
1718384435931381760 |