Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats
Abstract Acute pancreatitis (AP) is a common acute abdominal disease, 10–20% of which can evolve into severe AP (SAP) causing significant morbidity and mortality. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential of repairing SAP, but the detailed mechanism remains unknown. We de...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/bfb8527fd5b047f3b1734961b5c705dc |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:bfb8527fd5b047f3b1734961b5c705dc |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:bfb8527fd5b047f3b1734961b5c705dc2021-12-02T15:05:13ZBone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats10.1038/s41598-017-00629-32045-2322https://doaj.org/article/bfb8527fd5b047f3b1734961b5c705dc2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00629-3https://doaj.org/toc/2045-2322Abstract Acute pancreatitis (AP) is a common acute abdominal disease, 10–20% of which can evolve into severe AP (SAP) causing significant morbidity and mortality. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential of repairing SAP, but the detailed mechanism remains unknown. We demonstrate here that microRNA-9 (miR-9) modified BMSCs (pri-miR-9-BMSCs) can significantly reduce the pancreatic edema, infiltration, hemorrhage, necrosis, the release of amylase and lipase. Meanwhile, decreased local/systemic inflammatory response (TNF-α↓, IL-1β↓, IL-6↓, HMGB1↓, MPO↓, CD68↓, IL-4↑, IL-10↑, and TGF-β↑) and enhanced regeneration of damaged pancreas (Reg4↑, PTF1↑, and PDX1↑) are also promoted. But these effects diminish or disappear after antagonizing miR-9 (TuD). Besides, we find that miR-9 is negatively correlated with AP and miR-9 agomir which can mimic the effects of pri-miR-9-BMSCs and protect injured pancreas. Furthermore, we investigate that BMSCs deliver miR-9 to the injured pancreas or peripheral blood mononuclear cell (PBMC), which can target the NF-κB1/p50 gene and inhibit the NF-κB signaling pathway (p-P65↓, NF-κB1/p50↓, IκBα↑, IκBβ↑). Taken together, these results show that miR-9 is a key paracrine factor of BMSCs attenuating SAP targeting the NF-κB1/p50 gene and suppressing the NF-κB signaling pathway.Daohai QianGe WeiChenglei XuZhigang HeJie HuaJian LiQili HuShengping LinJian GongHongbo MengBo ZhouHongfei TengZhenshun SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Daohai Qian Ge Wei Chenglei Xu Zhigang He Jie Hua Jian Li Qili Hu Shengping Lin Jian Gong Hongbo Meng Bo Zhou Hongfei Teng Zhenshun Song Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats |
| description |
Abstract Acute pancreatitis (AP) is a common acute abdominal disease, 10–20% of which can evolve into severe AP (SAP) causing significant morbidity and mortality. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential of repairing SAP, but the detailed mechanism remains unknown. We demonstrate here that microRNA-9 (miR-9) modified BMSCs (pri-miR-9-BMSCs) can significantly reduce the pancreatic edema, infiltration, hemorrhage, necrosis, the release of amylase and lipase. Meanwhile, decreased local/systemic inflammatory response (TNF-α↓, IL-1β↓, IL-6↓, HMGB1↓, MPO↓, CD68↓, IL-4↑, IL-10↑, and TGF-β↑) and enhanced regeneration of damaged pancreas (Reg4↑, PTF1↑, and PDX1↑) are also promoted. But these effects diminish or disappear after antagonizing miR-9 (TuD). Besides, we find that miR-9 is negatively correlated with AP and miR-9 agomir which can mimic the effects of pri-miR-9-BMSCs and protect injured pancreas. Furthermore, we investigate that BMSCs deliver miR-9 to the injured pancreas or peripheral blood mononuclear cell (PBMC), which can target the NF-κB1/p50 gene and inhibit the NF-κB signaling pathway (p-P65↓, NF-κB1/p50↓, IκBα↑, IκBβ↑). Taken together, these results show that miR-9 is a key paracrine factor of BMSCs attenuating SAP targeting the NF-κB1/p50 gene and suppressing the NF-κB signaling pathway. |
| format |
article |
| author |
Daohai Qian Ge Wei Chenglei Xu Zhigang He Jie Hua Jian Li Qili Hu Shengping Lin Jian Gong Hongbo Meng Bo Zhou Hongfei Teng Zhenshun Song |
| author_facet |
Daohai Qian Ge Wei Chenglei Xu Zhigang He Jie Hua Jian Li Qili Hu Shengping Lin Jian Gong Hongbo Meng Bo Zhou Hongfei Teng Zhenshun Song |
| author_sort |
Daohai Qian |
| title |
Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats |
| title_short |
Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats |
| title_full |
Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats |
| title_fullStr |
Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats |
| title_full_unstemmed |
Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats |
| title_sort |
bone marrow-derived mesenchymal stem cells (bmscs) repair acute necrotized pancreatitis by secreting microrna-9 to target the nf-κb1/p50 gene in rats |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/bfb8527fd5b047f3b1734961b5c705dc |
| work_keys_str_mv |
AT daohaiqian bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT gewei bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT chengleixu bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT zhiganghe bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT jiehua bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT jianli bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT qilihu bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT shengpinglin bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT jiangong bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT hongbomeng bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT bozhou bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT hongfeiteng bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats AT zhenshunsong bonemarrowderivedmesenchymalstemcellsbmscsrepairacutenecrotizedpancreatitisbysecretingmicrorna9totargetthenfkb1p50geneinrats |
| _version_ |
1718388919558471680 |