Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China

<i>Giardia duodenalis</i> is a flagellated zoonotic parasite that can infect various animals and humans, causing economic losses in husbandry and detriments to public health. Although it has been reported in pigs worldwide, there are few reports on the prevalence and assemblages of <i...

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Autores principales:	Yang Zou, Xiao-Dan Yuan, Sheng-Ying Zhang, Hong-Yan Zhang, Xiao-Qing Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Giardia duodenalis</i> prevalence risk factors farmed pigs assemblages southern China Medicine R
Acceso en línea:	https://doaj.org/article/bfb91abf5e6d49cfb06efdc735e42d7f
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spelling	oai:doaj.org-article:bfb91abf5e6d49cfb06efdc735e42d7f2021-11-25T18:38:35ZMolecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China10.3390/pathogens101114812076-0817https://doaj.org/article/bfb91abf5e6d49cfb06efdc735e42d7f2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1481https://doaj.org/toc/2076-0817<i>Giardia duodenalis</i> is a flagellated zoonotic parasite that can infect various animals and humans, causing economic losses in husbandry and detriments to public health. Although it has been reported in pigs worldwide, there are few reports on the prevalence and assemblages of <i>G. duodenalis</i> infection in pigs in China. In this study, the 396 pig fecal samples were randomly collected from seven farms in Zhejiang, Guangdong and Yunnan provinces in southern China, and were examined by means of the nested PCR amplification of β-giardin (bg), glutamate dehydrogenase (gdh), and triose phosphate isomerase (tpi) for the detection of <i>G. duodenalis</i>. Overall, 21 fecal samples were positive for <i>G. duodenalis</i>, with a prevalence of 5.3%. Three risk factors are associated with <i>G. duodenalis</i> infection, namely, region, age and gender. Moreover, 13, six and two samples were successfully amplified at the bg, gdh and tpi gene loci, respectively. Three assemblages of <i>G. duodenalis</i> were identified, including assemblage E (<i>n</i> = 17), assemblage A (<i>n</i> = 3) and assemblage B (<i>n</i> = 1). Assemblage E was the dominating genotype and was distributed in three provinces. These assemblages of <i>G. duodenalis</i> have also been found in human beings, non-human primates, sheep, goats and cattle, which further reveals that farmed pigs pose a potential threat to public health.Yang ZouXiao-Dan YuanSheng-Ying ZhangHong-Yan ZhangXiao-Qing ChenMDPI AGarticle<i>Giardia duodenalis</i>prevalencerisk factorsfarmed pigsassemblagessouthern ChinaMedicineRENPathogens, Vol 10, Iss 1481, p 1481 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Giardia duodenalis</i> prevalence risk factors farmed pigs assemblages southern China Medicine R
spellingShingle	<i>Giardia duodenalis</i> prevalence risk factors farmed pigs assemblages southern China Medicine R Yang Zou Xiao-Dan Yuan Sheng-Ying Zhang Hong-Yan Zhang Xiao-Qing Chen Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China
description	<i>Giardia duodenalis</i> is a flagellated zoonotic parasite that can infect various animals and humans, causing economic losses in husbandry and detriments to public health. Although it has been reported in pigs worldwide, there are few reports on the prevalence and assemblages of <i>G. duodenalis</i> infection in pigs in China. In this study, the 396 pig fecal samples were randomly collected from seven farms in Zhejiang, Guangdong and Yunnan provinces in southern China, and were examined by means of the nested PCR amplification of β-giardin (bg), glutamate dehydrogenase (gdh), and triose phosphate isomerase (tpi) for the detection of <i>G. duodenalis</i>. Overall, 21 fecal samples were positive for <i>G. duodenalis</i>, with a prevalence of 5.3%. Three risk factors are associated with <i>G. duodenalis</i> infection, namely, region, age and gender. Moreover, 13, six and two samples were successfully amplified at the bg, gdh and tpi gene loci, respectively. Three assemblages of <i>G. duodenalis</i> were identified, including assemblage E (<i>n</i> = 17), assemblage A (<i>n</i> = 3) and assemblage B (<i>n</i> = 1). Assemblage E was the dominating genotype and was distributed in three provinces. These assemblages of <i>G. duodenalis</i> have also been found in human beings, non-human primates, sheep, goats and cattle, which further reveals that farmed pigs pose a potential threat to public health.
format	article
author	Yang Zou Xiao-Dan Yuan Sheng-Ying Zhang Hong-Yan Zhang Xiao-Qing Chen
author_facet	Yang Zou Xiao-Dan Yuan Sheng-Ying Zhang Hong-Yan Zhang Xiao-Qing Chen
author_sort	Yang Zou
title	Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China
title_short	Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China
title_full	Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China
title_fullStr	Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China
title_full_unstemmed	Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China
title_sort	molecular detection and characterization of <i>giardia duodenalis</i> in farmed pigs in three provinces of southern china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bfb91abf5e6d49cfb06efdc735e42d7f
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Molecular Detection and Characterization of <i>Giardia duodenalis</i> in Farmed Pigs in Three Provinces of Southern China

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