Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations

This study aims to determine the effectiveness of IFN-? and progesterone measurement as the basis for pregnancy diagnosis in Aceh cows. The study used twelve cows aged 3-5 years, weighing 150-250 kg, clinically healthy and having normal reproduction. The estrous cycle was synchronized using prostagl...

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Autores principales:	Budianto Panjaitan, TONGKU NIZWAN SIREGAR, HAFIZUDDIN HAFIZUDDIN, ARMAN SAYUTI, MULYADI ADAM, TEUKU ARMANSYAH, SYAFRUDDIN SYAFRUDDIN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	aceh cattle, ifn-τ, pregnancy diagnosis, progesterone Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bfc011e4fad34f8aae1422048147c7db
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spelling	oai:doaj.org-article:bfc011e4fad34f8aae1422048147c7db2021-11-22T00:57:38ZComparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations1412-033X2085-472210.13057/biodiv/d220414https://doaj.org/article/bfc011e4fad34f8aae1422048147c7db2021-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7653https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722This study aims to determine the effectiveness of IFN-? and progesterone measurement as the basis for pregnancy diagnosis in Aceh cows. The study used twelve cows aged 3-5 years, weighing 150-250 kg, clinically healthy and having normal reproduction. The estrous cycle was synchronized using prostaglandin hormone (LutalyseTM, Pharmacia & Upjohn Company, Pfizer Inc., 25 mg/cow, i.m.) with multiple injection protocol (10-day interval). Heat detection was conducted in the morning (08.00 Western Indonesia Time) and evening (16.00 Western Indonesia Time). Cows were inseminated 12-18 hours after standing heat. Blood was collected on day 4, 5, 6, 7, 14, 15, 16, 17, and 18 post-insemination. Concentrations of IFN-? and progesterone were determined by the ELISA method. Cows with an interferon concentration >6 pg/mL and a progesterone concentration >2 ng/mL were considered pregnant. Pregnancy data were then compared with transrectal ultrasound at day 25 as the gold standard. Concentrations of IFN-? in pregnant and non-pregnant cows were significantly different on day 18 post-insemination, while progesterone concentration was significantly different on day 15. The accuracy of IFN-? pregnancy diagnosis at peak IFN-? expression was obtained on day 18 post-insemination, with an accuracy rate of 66.7%, a sensitivity rate of 60% and a specificity rate of 71.4%. Meanwhile, the accuracy of progesterone-based pregnancy diagnosis was obtained on day 17 post-insemination, with an accuracy rate of 91.6%, a sensitivity rate of 83.3% and a specificity rate of 100%. It was concluded that the diagnosis of pregnancy using progesterone measurement was more effective than IFN-? measurement.Budianto PanjaitanTONGKU NIZWAN SIREGARHAFIZUDDIN HAFIZUDDINARMAN SAYUTIMULYADI ADAMTEUKU ARMANSYAHSYAFRUDDIN SYAFRUDDINMBI & UNS Soloarticleaceh cattle, ifn-τ, pregnancy diagnosis, progesteroneBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aceh cattle, ifn-τ, pregnancy diagnosis, progesterone Biology (General) QH301-705.5
spellingShingle	aceh cattle, ifn-τ, pregnancy diagnosis, progesterone Biology (General) QH301-705.5 Budianto Panjaitan TONGKU NIZWAN SIREGAR HAFIZUDDIN HAFIZUDDIN ARMAN SAYUTI MULYADI ADAM TEUKU ARMANSYAH SYAFRUDDIN SYAFRUDDIN Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations
description	This study aims to determine the effectiveness of IFN-? and progesterone measurement as the basis for pregnancy diagnosis in Aceh cows. The study used twelve cows aged 3-5 years, weighing 150-250 kg, clinically healthy and having normal reproduction. The estrous cycle was synchronized using prostaglandin hormone (LutalyseTM, Pharmacia & Upjohn Company, Pfizer Inc., 25 mg/cow, i.m.) with multiple injection protocol (10-day interval). Heat detection was conducted in the morning (08.00 Western Indonesia Time) and evening (16.00 Western Indonesia Time). Cows were inseminated 12-18 hours after standing heat. Blood was collected on day 4, 5, 6, 7, 14, 15, 16, 17, and 18 post-insemination. Concentrations of IFN-? and progesterone were determined by the ELISA method. Cows with an interferon concentration >6 pg/mL and a progesterone concentration >2 ng/mL were considered pregnant. Pregnancy data were then compared with transrectal ultrasound at day 25 as the gold standard. Concentrations of IFN-? in pregnant and non-pregnant cows were significantly different on day 18 post-insemination, while progesterone concentration was significantly different on day 15. The accuracy of IFN-? pregnancy diagnosis at peak IFN-? expression was obtained on day 18 post-insemination, with an accuracy rate of 66.7%, a sensitivity rate of 60% and a specificity rate of 71.4%. Meanwhile, the accuracy of progesterone-based pregnancy diagnosis was obtained on day 17 post-insemination, with an accuracy rate of 91.6%, a sensitivity rate of 83.3% and a specificity rate of 100%. It was concluded that the diagnosis of pregnancy using progesterone measurement was more effective than IFN-? measurement.
format	article
author	Budianto Panjaitan TONGKU NIZWAN SIREGAR HAFIZUDDIN HAFIZUDDIN ARMAN SAYUTI MULYADI ADAM TEUKU ARMANSYAH SYAFRUDDIN SYAFRUDDIN
author_facet	Budianto Panjaitan TONGKU NIZWAN SIREGAR HAFIZUDDIN HAFIZUDDIN ARMAN SAYUTI MULYADI ADAM TEUKU ARMANSYAH SYAFRUDDIN SYAFRUDDIN
author_sort	Budianto Panjaitan
title	Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations
title_short	Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations
title_full	Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations
title_fullStr	Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations
title_full_unstemmed	Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations
title_sort	comparison of the effectiveness of pregnancy diagnosis in aceh cattle through measurement of interferon-tau and progesterone concentrations
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/bfc011e4fad34f8aae1422048147c7db
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Comparison of the effectiveness of pregnancy diagnosis in Aceh cattle through measurement of interferon-tau and progesterone concentrations

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