Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI

Abstract. Ratnasari J, Esyanti RR, Tan MI, Juliawaty LD, Shimma S. 2021. Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI. Biodiversitas 22: 1172-1178. Cryptobrachytone C is an active compound isolated from leaves of Cryptocarya pulchrinervia, an indigen...

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Autores principales:	Jujun Ratnasari, Rizkita Rachmi Esyanti, Marselina Irasonia Tan, Lia Dewi Juliawaty, Shuichi Shimma
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	cryptocarya pulchrinervia, cryptobrachytone c, maldi-msi, mass spectrometry. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bfc02064b5134b7caa4a722d324be851
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spelling	oai:doaj.org-article:bfc02064b5134b7caa4a722d324be8512021-11-22T00:56:37ZProfile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI1412-033X2085-472210.13057/biodiv/d220312https://doaj.org/article/bfc02064b5134b7caa4a722d324be8512021-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7468https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ratnasari J, Esyanti RR, Tan MI, Juliawaty LD, Shimma S. 2021. Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI. Biodiversitas 22: 1172-1178. Cryptobrachytone C is an active compound isolated from leaves of Cryptocarya pulchrinervia, an indigenous plant from Indonesia. Cryptobrachytone C is cytotoxic against P388 leukemia cancer cell lines. A profile of cryptobrachytone C accumulation in leaves based on physiological age is necessary to study cryptobrachytone C production in the plant since it has anticancer potential. In situ profiling of cryptobrachytone C accumulation in leaves was carried out by imaging techniques using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The cryptobrachytone C ionization efficiency was carried out by derivatization using Girard-T reagent and coating with ?-cyano-4-hydroxycinnamic acid (?-CHCA) matrix. Scanned leaves show higher accumulation intensity in the second leaf. Quantitative accumulation profiling was conducted using GC-MS, where the highest amount of cryptobrachytone C was on the second leaf at 87.07 ± 47.21 mg. This showed that the most effective isolation of cryptobrachytone C would be obtained from young leaves of Cryptocarya pulchrinervia. This profile would play a role in cryptobrachytone C production in-situ or ex-situ using tissue culture.Jujun RatnasariRizkita Rachmi EsyantiMarselina Irasonia TanLia Dewi JuliawatyShuichi ShimmaMBI & UNS Soloarticlecryptocarya pulchrinervia, cryptobrachytone c, maldi-msi, mass spectrometry.Biology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryptocarya pulchrinervia, cryptobrachytone c, maldi-msi, mass spectrometry. Biology (General) QH301-705.5
spellingShingle	cryptocarya pulchrinervia, cryptobrachytone c, maldi-msi, mass spectrometry. Biology (General) QH301-705.5 Jujun Ratnasari Rizkita Rachmi Esyanti Marselina Irasonia Tan Lia Dewi Juliawaty Shuichi Shimma Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI
description	Abstract. Ratnasari J, Esyanti RR, Tan MI, Juliawaty LD, Shimma S. 2021. Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI. Biodiversitas 22: 1172-1178. Cryptobrachytone C is an active compound isolated from leaves of Cryptocarya pulchrinervia, an indigenous plant from Indonesia. Cryptobrachytone C is cytotoxic against P388 leukemia cancer cell lines. A profile of cryptobrachytone C accumulation in leaves based on physiological age is necessary to study cryptobrachytone C production in the plant since it has anticancer potential. In situ profiling of cryptobrachytone C accumulation in leaves was carried out by imaging techniques using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). The cryptobrachytone C ionization efficiency was carried out by derivatization using Girard-T reagent and coating with ?-cyano-4-hydroxycinnamic acid (?-CHCA) matrix. Scanned leaves show higher accumulation intensity in the second leaf. Quantitative accumulation profiling was conducted using GC-MS, where the highest amount of cryptobrachytone C was on the second leaf at 87.07 ± 47.21 mg. This showed that the most effective isolation of cryptobrachytone C would be obtained from young leaves of Cryptocarya pulchrinervia. This profile would play a role in cryptobrachytone C production in-situ or ex-situ using tissue culture.
format	article
author	Jujun Ratnasari Rizkita Rachmi Esyanti Marselina Irasonia Tan Lia Dewi Juliawaty Shuichi Shimma
author_facet	Jujun Ratnasari Rizkita Rachmi Esyanti Marselina Irasonia Tan Lia Dewi Juliawaty Shuichi Shimma
author_sort	Jujun Ratnasari
title	Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI
title_short	Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI
title_full	Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI
title_fullStr	Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI
title_full_unstemmed	Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI
title_sort	profile of cryptobrachytone c accumulation in cryptocarya pulchrinervia leaves using maldi-msi
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/bfc02064b5134b7caa4a722d324be851
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Profile of cryptobrachytone C accumulation in Cryptocarya pulchrinervia leaves using MALDI-MSI

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