Sarcoptes scabiei mites modulate gene expression in human skin equivalents.

The ectoparasitic mite, Sarcoptes scabiei that burrows in the epidermis of mammalian skin has a long co-evolution with its hosts. Phenotypic studies show that the mites have the ability to modulate cytokine secretion and expression of cell adhesion molecules in cells of the skin and other cells of t...

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Autores principales:	Marjorie S Morgan, Larry G Arlian, Michael P Markey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bfc400893b71473b945028465448c8ad
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spelling	oai:doaj.org-article:bfc400893b71473b945028465448c8ad2021-11-18T09:01:11ZSarcoptes scabiei mites modulate gene expression in human skin equivalents.1932-620310.1371/journal.pone.0071143https://doaj.org/article/bfc400893b71473b945028465448c8ad2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23940705/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The ectoparasitic mite, Sarcoptes scabiei that burrows in the epidermis of mammalian skin has a long co-evolution with its hosts. Phenotypic studies show that the mites have the ability to modulate cytokine secretion and expression of cell adhesion molecules in cells of the skin and other cells of the innate and adaptive immune systems that may assist the mites to survive in the skin. The purpose of this study was to identify genes in keratinocytes and fibroblasts in human skin equivalents (HSEs) that changed expression in response to the burrowing of live scabies mites. Overall, of the more than 25,800 genes measured, 189 genes were up-regulated >2-fold in response to scabies mite burrowing while 152 genes were down-regulated to the same degree. HSEs differentially expressed large numbers of genes that were related to host protective responses including those involved in immune response, defense response, cytokine activity, taxis, response to other organisms, and cell adhesion. Genes for the expression of interleukin-1α (IL-1α) precursor, IL-1β, granulocyte/macrophage-colony stimulating factor (GM-CSF) precursor, and G-CSF precursor were up-regulated 2.8- to 7.4-fold, paralleling cytokine secretion profiles. A large number of genes involved in epithelium development and keratinization were also differentially expressed in response to live scabies mites. Thus, these skin cells are directly responding as expected in an inflammatory response to products of the mites and the disruption of the skin's protective barrier caused by burrowing. This suggests that in vivo the interplay among these skin cells and other cell types, including Langerhans cells, dendritic cells, lymphocytes and endothelial cells, is responsible for depressing the host's protective response allowing these mites to survive in the skin.Marjorie S MorganLarry G ArlianMichael P MarkeyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71143 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marjorie S Morgan Larry G Arlian Michael P Markey Sarcoptes scabiei mites modulate gene expression in human skin equivalents.
description	The ectoparasitic mite, Sarcoptes scabiei that burrows in the epidermis of mammalian skin has a long co-evolution with its hosts. Phenotypic studies show that the mites have the ability to modulate cytokine secretion and expression of cell adhesion molecules in cells of the skin and other cells of the innate and adaptive immune systems that may assist the mites to survive in the skin. The purpose of this study was to identify genes in keratinocytes and fibroblasts in human skin equivalents (HSEs) that changed expression in response to the burrowing of live scabies mites. Overall, of the more than 25,800 genes measured, 189 genes were up-regulated >2-fold in response to scabies mite burrowing while 152 genes were down-regulated to the same degree. HSEs differentially expressed large numbers of genes that were related to host protective responses including those involved in immune response, defense response, cytokine activity, taxis, response to other organisms, and cell adhesion. Genes for the expression of interleukin-1α (IL-1α) precursor, IL-1β, granulocyte/macrophage-colony stimulating factor (GM-CSF) precursor, and G-CSF precursor were up-regulated 2.8- to 7.4-fold, paralleling cytokine secretion profiles. A large number of genes involved in epithelium development and keratinization were also differentially expressed in response to live scabies mites. Thus, these skin cells are directly responding as expected in an inflammatory response to products of the mites and the disruption of the skin's protective barrier caused by burrowing. This suggests that in vivo the interplay among these skin cells and other cell types, including Langerhans cells, dendritic cells, lymphocytes and endothelial cells, is responsible for depressing the host's protective response allowing these mites to survive in the skin.
format	article
author	Marjorie S Morgan Larry G Arlian Michael P Markey
author_facet	Marjorie S Morgan Larry G Arlian Michael P Markey
author_sort	Marjorie S Morgan
title	Sarcoptes scabiei mites modulate gene expression in human skin equivalents.
title_short	Sarcoptes scabiei mites modulate gene expression in human skin equivalents.
title_full	Sarcoptes scabiei mites modulate gene expression in human skin equivalents.
title_fullStr	Sarcoptes scabiei mites modulate gene expression in human skin equivalents.
title_full_unstemmed	Sarcoptes scabiei mites modulate gene expression in human skin equivalents.
title_sort	sarcoptes scabiei mites modulate gene expression in human skin equivalents.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bfc400893b71473b945028465448c8ad
work_keys_str_mv	AT marjoriesmorgan sarcoptesscabieimitesmodulategeneexpressioninhumanskinequivalents AT larrygarlian sarcoptesscabieimitesmodulategeneexpressioninhumanskinequivalents AT michaelpmarkey sarcoptesscabieimitesmodulategeneexpressioninhumanskinequivalents
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Sarcoptes scabiei mites modulate gene expression in human skin equivalents.

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