Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>

ABSTRACT Microbial conversion of carbon dioxide to organic commodities via syngas metabolism or microbial electrosynthesis is an attractive option for production of renewable biocommodities. The recent development of an initial genetic toolbox for the acetogen Clostridium ljungdahlii has suggested t...

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Autores principales:	Toshiyuki Ueki, Kelly P. Nevin, Trevor L. Woodard, Derek R. Lovley
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/bfd3b1b59aa444e4a9fdf456d25fec3b
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spelling	oai:doaj.org-article:bfd3b1b59aa444e4a9fdf456d25fec3b2021-11-15T15:45:54ZConverting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>10.1128/mBio.01636-142150-7511https://doaj.org/article/bfd3b1b59aa444e4a9fdf456d25fec3b2014-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01636-14https://doaj.org/toc/2150-7511ABSTRACT Microbial conversion of carbon dioxide to organic commodities via syngas metabolism or microbial electrosynthesis is an attractive option for production of renewable biocommodities. The recent development of an initial genetic toolbox for the acetogen Clostridium ljungdahlii has suggested that C. ljungdahlii may be an effective chassis for such conversions. This possibility was evaluated by engineering a strain to produce butyrate, a valuable commodity that is not a natural product of C. ljungdahlii metabolism. Heterologous genes required for butyrate production from acetyl-coenzyme A (CoA) were identified and introduced initially on plasmids and in subsequent strain designs integrated into the C. ljungdahlii chromosome. Iterative strain designs involved increasing translation of a key enzyme by modifying a ribosome binding site, inactivating the gene encoding the first step in the conversion of acetyl-CoA to acetate, disrupting the gene which encodes the primary bifunctional aldehyde/alcohol dehydrogenase for ethanol production, and interrupting the gene for a CoA transferase that potentially represented an alternative route for the production of acetate. These modifications yielded a strain in which ca. 50 or 70% of the carbon and electron flow was diverted to the production of butyrate with H2 or CO as the electron donor, respectively. These results demonstrate the possibility of producing high-value commodities from carbon dioxide with C. ljungdahlii as the catalyst. IMPORTANCE The development of a microbial chassis for efficient conversion of carbon dioxide directly to desired organic products would greatly advance the environmentally sustainable production of biofuels and other commodities. Clostridium ljungdahlii is an effective catalyst for microbial electrosynthesis, a technology in which electricity generated with renewable technologies, such as solar or wind, powers the conversion of carbon dioxide and water to organic products. Other electron donors for C. ljungdahlii include carbon monoxide, which can be derived from industrial waste gases or the conversion of recalcitrant biomass to syngas, as well as hydrogen, another syngas component. The finding that carbon and electron flow in C. ljungdahlii can be diverted from the production of acetate to butyrate synthesis is an important step toward the goal of renewable commodity production from carbon dioxide with this organism.Toshiyuki UekiKelly P. NevinTrevor L. WoodardDerek R. LovleyAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 5 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Toshiyuki Ueki Kelly P. Nevin Trevor L. Woodard Derek R. Lovley Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>
description	ABSTRACT Microbial conversion of carbon dioxide to organic commodities via syngas metabolism or microbial electrosynthesis is an attractive option for production of renewable biocommodities. The recent development of an initial genetic toolbox for the acetogen Clostridium ljungdahlii has suggested that C. ljungdahlii may be an effective chassis for such conversions. This possibility was evaluated by engineering a strain to produce butyrate, a valuable commodity that is not a natural product of C. ljungdahlii metabolism. Heterologous genes required for butyrate production from acetyl-coenzyme A (CoA) were identified and introduced initially on plasmids and in subsequent strain designs integrated into the C. ljungdahlii chromosome. Iterative strain designs involved increasing translation of a key enzyme by modifying a ribosome binding site, inactivating the gene encoding the first step in the conversion of acetyl-CoA to acetate, disrupting the gene which encodes the primary bifunctional aldehyde/alcohol dehydrogenase for ethanol production, and interrupting the gene for a CoA transferase that potentially represented an alternative route for the production of acetate. These modifications yielded a strain in which ca. 50 or 70% of the carbon and electron flow was diverted to the production of butyrate with H2 or CO as the electron donor, respectively. These results demonstrate the possibility of producing high-value commodities from carbon dioxide with C. ljungdahlii as the catalyst. IMPORTANCE The development of a microbial chassis for efficient conversion of carbon dioxide directly to desired organic products would greatly advance the environmentally sustainable production of biofuels and other commodities. Clostridium ljungdahlii is an effective catalyst for microbial electrosynthesis, a technology in which electricity generated with renewable technologies, such as solar or wind, powers the conversion of carbon dioxide and water to organic products. Other electron donors for C. ljungdahlii include carbon monoxide, which can be derived from industrial waste gases or the conversion of recalcitrant biomass to syngas, as well as hydrogen, another syngas component. The finding that carbon and electron flow in C. ljungdahlii can be diverted from the production of acetate to butyrate synthesis is an important step toward the goal of renewable commodity production from carbon dioxide with this organism.
format	article
author	Toshiyuki Ueki Kelly P. Nevin Trevor L. Woodard Derek R. Lovley
author_facet	Toshiyuki Ueki Kelly P. Nevin Trevor L. Woodard Derek R. Lovley
author_sort	Toshiyuki Ueki
title	Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>
title_short	Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>
title_full	Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>
title_fullStr	Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>
title_full_unstemmed	Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>
title_sort	converting carbon dioxide to butyrate with an engineered strain of <named-content content-type="genus-species">clostridium ljungdahlii</named-content>
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/bfd3b1b59aa444e4a9fdf456d25fec3b
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Converting Carbon Dioxide to Butyrate with an Engineered Strain of <named-content content-type="genus-species">Clostridium ljungdahlii</named-content>

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