Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization

Abstract. Putri RE, Mubarik NR, Ambarsari L, Wahyudi AT. 2021. Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization. Biodiversitas 22: 4067-4077. Biocontrol of Fusarium oxysporum, a phytopathogenic fungus that causes plant...

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Autores principales:	Rury Eryna Putri, Nisa Rachmania Mubarik, Laksmi Ambarsari, Aris Tri Wahyudi
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	ammonium sulphate faction b. velezensis food poisoned assay fungal biomass Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/bfd774e2e75544278258c7182ced2cb1
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spelling	oai:doaj.org-article:bfd774e2e75544278258c7182ced2cb12021-11-22T12:18:55ZAntagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization1412-033X2085-472210.13057/biodiv/d220956https://doaj.org/article/bfd774e2e75544278258c7182ced2cb12021-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8990https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Putri RE, Mubarik NR, Ambarsari L, Wahyudi AT. 2021. Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization. Biodiversitas 22: 4067-4077. Biocontrol of Fusarium oxysporum, a phytopathogenic fungus that causes plant wilt can be approached with cell-wall degrading enzymes such as ?-glucanase. The aim of this study was to evaluate the prospective ability in glucanase production from several soil bacterial isolates and to characterize its ?-glucanase activity of ammonium sulfate precipitation, and to determine its antifungal activity against F. oxysporum in vitro. Twenty bacterial isolates were screened qualitatively and quantitatively as ?-glucanase producers. The results showed that the prospective isolate W3.15 can produce ?-glucanase on glucan agar as the selection medium. From 16S rRNA sequences identification, the isolate belongs to the genus Bacillus, closely related to Bacillus subtilis. The enzyme activity of the ammonium sulfate fraction of isolate W3.15 is optimum at a pH of 7 and temperature range of 60-80oC. B. subtilis W3.15 exhibits high inhibition against the mycelial growth of F. oxysporum and significantly reduced fungal biomass.Rury Eryna PutriNisa Rachmania MubarikLaksmi AmbarsariAris Tri WahyudiMBI & UNS Soloarticleammonium sulphate factionb. velezensisfood poisoned assayfungal biomassBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ammonium sulphate faction b. velezensis food poisoned assay fungal biomass Biology (General) QH301-705.5
spellingShingle	ammonium sulphate faction b. velezensis food poisoned assay fungal biomass Biology (General) QH301-705.5 Rury Eryna Putri Nisa Rachmania Mubarik Laksmi Ambarsari Aris Tri Wahyudi Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization
description	Abstract. Putri RE, Mubarik NR, Ambarsari L, Wahyudi AT. 2021. Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization. Biodiversitas 22: 4067-4077. Biocontrol of Fusarium oxysporum, a phytopathogenic fungus that causes plant wilt can be approached with cell-wall degrading enzymes such as ?-glucanase. The aim of this study was to evaluate the prospective ability in glucanase production from several soil bacterial isolates and to characterize its ?-glucanase activity of ammonium sulfate precipitation, and to determine its antifungal activity against F. oxysporum in vitro. Twenty bacterial isolates were screened qualitatively and quantitatively as ?-glucanase producers. The results showed that the prospective isolate W3.15 can produce ?-glucanase on glucan agar as the selection medium. From 16S rRNA sequences identification, the isolate belongs to the genus Bacillus, closely related to Bacillus subtilis. The enzyme activity of the ammonium sulfate fraction of isolate W3.15 is optimum at a pH of 7 and temperature range of 60-80oC. B. subtilis W3.15 exhibits high inhibition against the mycelial growth of F. oxysporum and significantly reduced fungal biomass.
format	article
author	Rury Eryna Putri Nisa Rachmania Mubarik Laksmi Ambarsari Aris Tri Wahyudi
author_facet	Rury Eryna Putri Nisa Rachmania Mubarik Laksmi Ambarsari Aris Tri Wahyudi
author_sort	Rury Eryna Putri
title	Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization
title_short	Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization
title_full	Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization
title_fullStr	Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization
title_full_unstemmed	Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization
title_sort	antagonistic activity of glucanolytic bacteria bacillus subtilis w3.15 against fusarium oxysporum and its enzyme characterization
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/bfd774e2e75544278258c7182ced2cb1
work_keys_str_mv	AT ruryerynaputri antagonisticactivityofglucanolyticbacteriabacillussubtilisw315againstfusariumoxysporumanditsenzymecharacterization AT nisarachmaniamubarik antagonisticactivityofglucanolyticbacteriabacillussubtilisw315againstfusariumoxysporumanditsenzymecharacterization AT laksmiambarsari antagonisticactivityofglucanolyticbacteriabacillussubtilisw315againstfusariumoxysporumanditsenzymecharacterization AT aristriwahyudi antagonisticactivityofglucanolyticbacteriabacillussubtilisw315againstfusariumoxysporumanditsenzymecharacterization
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Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization

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