A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin

Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependen...

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Autores principales:	Nang Noon Shean Aye, Pornsuda Maraming, Ratree Tavichakorntrakool, Attawut Chaibunruang, Patcharee Boonsiri, Sakda Daduang, Nattiya Teawtrakul, Prinya Prasongdee, Vittaya Amornkitbamrung, Jureerut Daduang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electrochemical aptasensor graphene oxide diabetes mellitus glycated albumin Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/bfddcb40f8ed40b297ca5b211cea125e
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spelling	oai:doaj.org-article:bfddcb40f8ed40b297ca5b211cea125e2021-11-11T15:20:25ZA Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin10.3390/app1121103152076-3417https://doaj.org/article/bfddcb40f8ed40b297ca5b211cea125e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10315https://doaj.org/toc/2076-3417Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependent on the covalent interaction of the amine-functionalized GA-specific aptamer with the carboxylic groups of GO. Square wave voltammetry (SWV) analysis was carried out for the measurement of GA-aptamer binding to their specific proteins. The peak current changes before and after incubation with GA protein were directly proportional to the concentration. The developed aptasensor exhibited a broad linearity (1–10,000 µg mL<sup>−1</sup>), a low detection limit (LOD) of 0.031 µg mL<sup>−1</sup>, and high selectivity for GA detection. In addition, the aptasensor was successfully applied to detect GA in both spiked and clinical serum samples. The comparison of the developed method with a commercial assay validated the reliability of the aptasensor for clinical application. Therefore, the newly developed aptasensor is a promising tool for GA measurements in diabetic patients with underlying thalassemia.Nang Noon Shean AyePornsuda MaramingRatree TavichakorntrakoolAttawut ChaibunruangPatcharee BoonsiriSakda DaduangNattiya TeawtrakulPrinya PrasongdeeVittaya AmornkitbamrungJureerut DaduangMDPI AGarticleelectrochemical aptasensorgraphene oxidediabetes mellitusglycated albuminTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10315, p 10315 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrochemical aptasensor graphene oxide diabetes mellitus glycated albumin Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	electrochemical aptasensor graphene oxide diabetes mellitus glycated albumin Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Nang Noon Shean Aye Pornsuda Maraming Ratree Tavichakorntrakool Attawut Chaibunruang Patcharee Boonsiri Sakda Daduang Nattiya Teawtrakul Prinya Prasongdee Vittaya Amornkitbamrung Jureerut Daduang A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin
description	Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependent on the covalent interaction of the amine-functionalized GA-specific aptamer with the carboxylic groups of GO. Square wave voltammetry (SWV) analysis was carried out for the measurement of GA-aptamer binding to their specific proteins. The peak current changes before and after incubation with GA protein were directly proportional to the concentration. The developed aptasensor exhibited a broad linearity (1–10,000 µg mL<sup>−1</sup>), a low detection limit (LOD) of 0.031 µg mL<sup>−1</sup>, and high selectivity for GA detection. In addition, the aptasensor was successfully applied to detect GA in both spiked and clinical serum samples. The comparison of the developed method with a commercial assay validated the reliability of the aptasensor for clinical application. Therefore, the newly developed aptasensor is a promising tool for GA measurements in diabetic patients with underlying thalassemia.
format	article
author	Nang Noon Shean Aye Pornsuda Maraming Ratree Tavichakorntrakool Attawut Chaibunruang Patcharee Boonsiri Sakda Daduang Nattiya Teawtrakul Prinya Prasongdee Vittaya Amornkitbamrung Jureerut Daduang
author_facet	Nang Noon Shean Aye Pornsuda Maraming Ratree Tavichakorntrakool Attawut Chaibunruang Patcharee Boonsiri Sakda Daduang Nattiya Teawtrakul Prinya Prasongdee Vittaya Amornkitbamrung Jureerut Daduang
author_sort	Nang Noon Shean Aye
title	A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin
title_short	A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin
title_full	A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin
title_fullStr	A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin
title_full_unstemmed	A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin
title_sort	simple graphene functionalized electrochemical aptasensor for the sensitive and selective detection of glycated albumin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/bfddcb40f8ed40b297ca5b211cea125e
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A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin

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