Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties

Emerging research suggests gut microbiome may play a role in pancreatic cancer initiation and progression, but cultivation of the cancer microbiome remains challenging. This pilot study aims to investigate the possibility to cultivate pancreatic microbiome from pancreatic cystic lesions associated w...

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Autores principales:	Asif Halimi, Giorgio Gabarrini, Michał Jacek Sobkowiak, Zeeshan Ateeb, Haleh Davanian, Rogier Aäron Gaiser, Urban Arnelo, Roberto Valente, Alicia Y.W. Wong, Carlos Fernández Moro, Marco Del Chiaro, Volkan Özenci, Margaret Sällberg Chen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	pancreatic cancer pancreatic cystic neoplasm ipmn microbiota Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/bfde9b697bbd44be85f2449ebb9f20c2
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spelling	oai:doaj.org-article:bfde9b697bbd44be85f2449ebb9f20c22021-11-26T11:19:48ZIsolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties1949-09761949-098410.1080/19490976.2021.1983101https://doaj.org/article/bfde9b697bbd44be85f2449ebb9f20c22021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/19490976.2021.1983101https://doaj.org/toc/1949-0976https://doaj.org/toc/1949-0984Emerging research suggests gut microbiome may play a role in pancreatic cancer initiation and progression, but cultivation of the cancer microbiome remains challenging. This pilot study aims to investigate the possibility to cultivate pancreatic microbiome from pancreatic cystic lesions associated with invasive cancer. Intra-operatively acquired pancreatic cyst fluid samples showed culture-positivity mainly in the intraductal papillary mucinous neoplasm (IPMN) group of lesions. MALDI-TOF MS profiling analysis shows Gammaproteobacteria and Bacilli dominate among individual bacteria isolates. Among cultivated bacteria, Gammaproteobacteria, particularly Klebsiella pneumoniae, but also Granulicatella adiacens and Enterococcus faecalis, demonstrate consistent pathogenic properties in pancreatic cell lines tested in ex vivo co-culture models. Pathogenic properties include intracellular survival capability, cell death induction, or causing DNA double-strand breaks in the surviving cells resembling genotoxic effects. This study provides new insights into the role of the pancreatic microbiota in the intriguing link between pancreatic cystic lesions and cancer.Asif HalimiGiorgio GabarriniMichał Jacek SobkowiakZeeshan AteebHaleh DavanianRogier Aäron GaiserUrban ArneloRoberto ValenteAlicia Y.W. WongCarlos Fernández MoroMarco Del ChiaroVolkan ÖzenciMargaret Sällberg ChenTaylor & Francis Grouparticlepancreatic cancerpancreatic cystic neoplasmipmnmicrobiotaDiseases of the digestive system. GastroenterologyRC799-869ENGut Microbes, Vol 13, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pancreatic cancer pancreatic cystic neoplasm ipmn microbiota Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	pancreatic cancer pancreatic cystic neoplasm ipmn microbiota Diseases of the digestive system. Gastroenterology RC799-869 Asif Halimi Giorgio Gabarrini Michał Jacek Sobkowiak Zeeshan Ateeb Haleh Davanian Rogier Aäron Gaiser Urban Arnelo Roberto Valente Alicia Y.W. Wong Carlos Fernández Moro Marco Del Chiaro Volkan Özenci Margaret Sällberg Chen Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties
description	Emerging research suggests gut microbiome may play a role in pancreatic cancer initiation and progression, but cultivation of the cancer microbiome remains challenging. This pilot study aims to investigate the possibility to cultivate pancreatic microbiome from pancreatic cystic lesions associated with invasive cancer. Intra-operatively acquired pancreatic cyst fluid samples showed culture-positivity mainly in the intraductal papillary mucinous neoplasm (IPMN) group of lesions. MALDI-TOF MS profiling analysis shows Gammaproteobacteria and Bacilli dominate among individual bacteria isolates. Among cultivated bacteria, Gammaproteobacteria, particularly Klebsiella pneumoniae, but also Granulicatella adiacens and Enterococcus faecalis, demonstrate consistent pathogenic properties in pancreatic cell lines tested in ex vivo co-culture models. Pathogenic properties include intracellular survival capability, cell death induction, or causing DNA double-strand breaks in the surviving cells resembling genotoxic effects. This study provides new insights into the role of the pancreatic microbiota in the intriguing link between pancreatic cystic lesions and cancer.
format	article
author	Asif Halimi Giorgio Gabarrini Michał Jacek Sobkowiak Zeeshan Ateeb Haleh Davanian Rogier Aäron Gaiser Urban Arnelo Roberto Valente Alicia Y.W. Wong Carlos Fernández Moro Marco Del Chiaro Volkan Özenci Margaret Sällberg Chen
author_facet	Asif Halimi Giorgio Gabarrini Michał Jacek Sobkowiak Zeeshan Ateeb Haleh Davanian Rogier Aäron Gaiser Urban Arnelo Roberto Valente Alicia Y.W. Wong Carlos Fernández Moro Marco Del Chiaro Volkan Özenci Margaret Sällberg Chen
author_sort	Asif Halimi
title	Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties
title_short	Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties
title_full	Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties
title_fullStr	Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties
title_full_unstemmed	Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties
title_sort	isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and dna damaging properties
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/bfde9b697bbd44be85f2449ebb9f20c2
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Isolation of pancreatic microbiota from cystic precursors of pancreatic cancer with intracellular growth and DNA damaging properties

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