An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila

Abstract Inversion polymorphism is widespread in the Drosophila genus as well as in other dipteran genera. The presence of polytene chromosomes in some insect organs and, thus, the possibility to observe the different arrangements generated by inversions through a microscope enhanced the cytological...

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Autores principales:	Dorcas J. Orengo, Eva Puerma, Unai Cereijo, David Salguero, Montserrat Aguadé
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bfe6ec610f894978926f13a58ab0d4e1
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spelling	oai:doaj.org-article:bfe6ec610f894978926f13a58ab0d4e12021-12-02T15:05:10ZAn easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila10.1038/s41598-017-13043-62045-2322https://doaj.org/article/bfe6ec610f894978926f13a58ab0d4e12017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13043-6https://doaj.org/toc/2045-2322Abstract Inversion polymorphism is widespread in the Drosophila genus as well as in other dipteran genera. The presence of polytene chromosomes in some insect organs and, thus, the possibility to observe the different arrangements generated by inversions through a microscope enhanced the cytological study of this structural polymorphism. In several Drosophila species, these studies provided evidence for the adaptive character of this polymorphism, which together with the standing interest to uncover targets of natural selection has led to a renewed interest for inversion polymorphism. Our recent molecular characterization of the breakpoint regions of five inversions of the E chromosome of D. subobscura has allowed us to design a PCR-based strategy to molecularly identify the different chromosomal arrangements and, most importantly, to determine the E chromosome karyotype of medium- and large-sized samples from natural populations. Individuals of a test sample that were both cytologically and molecularly karyotyped were used to establish the strategy that was subsequently applied to karyotype a larger sample. Our strategy has proved to be robust and time efficient, and it lays therefore the groundwork for future studies of the E chromosome structural polymorphism through space and time, and of its putative contribution to adaptation.Dorcas J. OrengoEva PuermaUnai CereijoDavid SalgueroMontserrat AguadéNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dorcas J. Orengo Eva Puerma Unai Cereijo David Salguero Montserrat Aguadé An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila
description	Abstract Inversion polymorphism is widespread in the Drosophila genus as well as in other dipteran genera. The presence of polytene chromosomes in some insect organs and, thus, the possibility to observe the different arrangements generated by inversions through a microscope enhanced the cytological study of this structural polymorphism. In several Drosophila species, these studies provided evidence for the adaptive character of this polymorphism, which together with the standing interest to uncover targets of natural selection has led to a renewed interest for inversion polymorphism. Our recent molecular characterization of the breakpoint regions of five inversions of the E chromosome of D. subobscura has allowed us to design a PCR-based strategy to molecularly identify the different chromosomal arrangements and, most importantly, to determine the E chromosome karyotype of medium- and large-sized samples from natural populations. Individuals of a test sample that were both cytologically and molecularly karyotyped were used to establish the strategy that was subsequently applied to karyotype a larger sample. Our strategy has proved to be robust and time efficient, and it lays therefore the groundwork for future studies of the E chromosome structural polymorphism through space and time, and of its putative contribution to adaptation.
format	article
author	Dorcas J. Orengo Eva Puerma Unai Cereijo David Salguero Montserrat Aguadé
author_facet	Dorcas J. Orengo Eva Puerma Unai Cereijo David Salguero Montserrat Aguadé
author_sort	Dorcas J. Orengo
title	An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila
title_short	An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila
title_full	An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila
title_fullStr	An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila
title_full_unstemmed	An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila
title_sort	easy route to the massive karyotyping of complex chromosomal arrangements in drosophila
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/bfe6ec610f894978926f13a58ab0d4e1
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An easy route to the massive karyotyping of complex chromosomal arrangements in Drosophila

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