The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.

Phage therapy may become a complement to antibiotics in the treatment of chronic Pseudomonas aeruginosa infection. To design efficient therapeutic cocktails, the genetic diversity of the species and the spectrum of susceptibility to bacteriophages must be investigated. Bacterial strains showing high...

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Autores principales:	Christiane Essoh, Yann Blouin, Guillaume Loukou, Arsher Cablanmian, Serge Lathro, Elizabeth Kutter, Hoang Vu Thien, Gilles Vergnaud, Christine Pourcel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/bfee257388a24b4c8fcd67fbefcab8f7
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spelling	oai:doaj.org-article:bfee257388a24b4c8fcd67fbefcab8f72021-11-18T07:48:08ZThe susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.1932-620310.1371/journal.pone.0060575https://doaj.org/article/bfee257388a24b4c8fcd67fbefcab8f72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637754/?tool=EBIhttps://doaj.org/toc/1932-6203Phage therapy may become a complement to antibiotics in the treatment of chronic Pseudomonas aeruginosa infection. To design efficient therapeutic cocktails, the genetic diversity of the species and the spectrum of susceptibility to bacteriophages must be investigated. Bacterial strains showing high levels of phage resistance need to be identified in order to decipher the underlying mechanisms. Here we have selected genetically diverse P. aeruginosa strains from cystic fibrosis patients and tested their susceptibility to a large collection of phages. Based on plaque morphology and restriction profiles, six different phages were purified from "pyophage", a commercial cocktail directed against five different bacterial species, including P. aeruginosa. Characterization of these phages by electron microscopy and sequencing of genome fragments showed that they belong to 4 different genera. Among 47 P. aeruginosa strains, 13 were not lysed by any of the isolated phages individually or by pyophage. We isolated two new phages that could lyse some of these strains, and their genomes were sequenced. The presence/absence of a CRISPR-Cas system (Clustered Regularly Interspaced Short Palindromic Repeats and Crisper associated genes) was investigated to evaluate the role of the system in phage resistance. Altogether, the results show that some P. aeruginosa strains cannot support the growth of any of the tested phages belonging to 5 different genera, and suggest that the CRISPR-Cas system is not a major defence mechanism against these lytic phages.Christiane EssohYann BlouinGuillaume LoukouArsher CablanmianSerge LathroElizabeth KutterHoang Vu ThienGilles VergnaudChristine PourcelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60575 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christiane Essoh Yann Blouin Guillaume Loukou Arsher Cablanmian Serge Lathro Elizabeth Kutter Hoang Vu Thien Gilles Vergnaud Christine Pourcel The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.
description	Phage therapy may become a complement to antibiotics in the treatment of chronic Pseudomonas aeruginosa infection. To design efficient therapeutic cocktails, the genetic diversity of the species and the spectrum of susceptibility to bacteriophages must be investigated. Bacterial strains showing high levels of phage resistance need to be identified in order to decipher the underlying mechanisms. Here we have selected genetically diverse P. aeruginosa strains from cystic fibrosis patients and tested their susceptibility to a large collection of phages. Based on plaque morphology and restriction profiles, six different phages were purified from "pyophage", a commercial cocktail directed against five different bacterial species, including P. aeruginosa. Characterization of these phages by electron microscopy and sequencing of genome fragments showed that they belong to 4 different genera. Among 47 P. aeruginosa strains, 13 were not lysed by any of the isolated phages individually or by pyophage. We isolated two new phages that could lyse some of these strains, and their genomes were sequenced. The presence/absence of a CRISPR-Cas system (Clustered Regularly Interspaced Short Palindromic Repeats and Crisper associated genes) was investigated to evaluate the role of the system in phage resistance. Altogether, the results show that some P. aeruginosa strains cannot support the growth of any of the tested phages belonging to 5 different genera, and suggest that the CRISPR-Cas system is not a major defence mechanism against these lytic phages.
format	article
author	Christiane Essoh Yann Blouin Guillaume Loukou Arsher Cablanmian Serge Lathro Elizabeth Kutter Hoang Vu Thien Gilles Vergnaud Christine Pourcel
author_facet	Christiane Essoh Yann Blouin Guillaume Loukou Arsher Cablanmian Serge Lathro Elizabeth Kutter Hoang Vu Thien Gilles Vergnaud Christine Pourcel
author_sort	Christiane Essoh
title	The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.
title_short	The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.
title_full	The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.
title_fullStr	The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.
title_full_unstemmed	The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.
title_sort	susceptibility of pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/bfee257388a24b4c8fcd67fbefcab8f7
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The susceptibility of Pseudomonas aeruginosa strains from cystic fibrosis patients to bacteriophages.

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